Central Muga Eri Research and Training Institute

Assam, India

Central Muga Eri Research and Training Institute

Assam, India
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Nayak T.,Indian Institute of Technology Kharagpur | Mandal S.M.,Indian Institute of Technology Kharagpur | Neog K.,Central Muga Eri Research and Training Institute | Ghosh A.K.,Indian Institute of Technology Kharagpur
International Journal of Peptide Research and Therapeutics | Year: 2017

Antimicrobial peptides (AMPs) are produced in all living organisms including insects in a non-specific manner, and act as innate immune defense arsenal against the invading pathogens. Muga silkworm (Antheraea assamensis) larvae were injected with Candida albicans and AMPs were isolated from the hemolymph after extracting with methanol, acetic acid and water mixture (90:1:9) and evaluated for antimicrobial activity against fungal and bacterial pathogens. Further purification was done through successive semipreparative and analytical reversed phase HPLC using C-18 column. The obtained fractions were collected, lyophilized and tested for antimicrobial activity. Among the HPLC fractions, one showed highest activity with MIC value of 64 µg/ml against Gram-negative bacteria, Escherichia coli and Enterobacter cloacae. Purity of this isolated peptide was confirmed by SDS-PAGE and TLC, and its molecular mass was determined as 9.052 kDa by MALDI-TOF mass spectrometry. From the mass fingerprinting analysis of this peptide after trypsin digestion a peptide fragment with molecular mass of 2622.7 Da was obtained. De novo sequencing of this peptide fragment following MS/MS analysis identified few amino acid residues as “KSGGGGWGS” with a total score of 46.9 with gloverin peptide of A. mylitta. The peptide inhibited biofilm formation of the Gram-negative bacterial pathogens. SEM study revealed that peptide disrupted bacterial cell wall to leach out intracellular materials and may be the major target for its antimicrobial activity. © 2017 Springer Science+Business Media, LLC

Neog K.,Central Muga Eri Research and Training Institute | Das A.,Central Muga Eri Research and Training Institute | Unni B.G.,North East Institute of Science and Technology | Ahmed G.U.,Gauhati University | Rajan R.K.,Central Muga Eri Research and Training Institute
International Journal of PharmTech Research | Year: 2011

The host plant selection behaviour or feeding preferences of the insects are largely mediated by the presence and distribution of secondary metabolites in plants. Quantitative estimation of few secondary metabolites and HPLC analysis of phenolic compounds were carried out from the leaves of Som plant (Persea bombycina Kost) which is a primary metabolite of the shimmering yellow golden silk yarn producer, the muga silkworm (Antheraea assamensis Helfer). β -sitosterol content in the tender leaves was significantly the highest (1.06%) over semi-mature or mature leaves, whereas Chlorogenic acid content was more in medium leaves (2.06%). Phytic acid content was significantly higher in mature leaves (2310.05 mg/100g), total phenol in tender leaves (1.946%), ADF in mature leaves (26.50%), ADL in mature leaves and tannin in tender leaves (6.71%). Purified phenolic extract when subjected to HPLC analysis, 6 prominent peaks were observed and screening with standard indicates Peaks 1, 2, 3 and 6 correspond to Chlorogenic acid, catechol, Morin and gallic acid, respectively.

PubMed | Indian Agricultural Research Institute, Central Sericultural Research & Training Institute and Central Muga Eri Research and Training Institute
Type: Journal Article | Journal: Zootaxa | Year: 2015

The parasitic lepidopteran insect, Fulgoraecia melanoleuca (Fletcher) has been reported as an ectoparasitoid of Pyrilla perpusilla (Walker) from the Indian subcontinent. For the first time, the complete morphology, field biology, egg laying behavior, larval pupal, and adult morphology, including male and female gentialic features, are described and illustrated.

Das R.,Central Muga Eri Research and Training Institute | Chutia M.,Central Muga Eri Research and Training Institute | Das K.,Central Muga Eri Research and Training Institute | Jha D.K.,Gauhati University
Crop Protection | Year: 2010

The effect of inoculum concentrations, temperature, relative humidity (RH), incubation period and leaf age on sporulation of Pestalotiopsis disseminata and the development of grey blight disease on som (Persea bombycina Kost.) were studied in controlled environmental conditions. These factors had a significant role on spore germination and germ tube development of P. disseminata, the causal organism of the disease. The optimum inoculum concentration of the pathogen were found to be 1 × 107 spores ml-1 for maximum infection percentage. A nonlinear relationship was found between temperature and germination percentage of spores at a given relative humidity (RH). However, 25 °C (±2) temperature and 70% RH were the optimum parameters for better spore germination of the pathogen. Young leaves (leaf no. 1-4 from the top) were more susceptible for development of the diseases in a controlled environment than the older leaves. The germination of spores started at 8 h of incubation and gradually increased up to 20 h (maximum). The disease severity was favoured in the optimum range of temperature (25 ± 2 °C) and RH (70%) which had a combined effect. These epidemiological parameters could help in the management practices of the disease. © 2010.

Chakravorty R.,Central Muga Eri Research and Training Institute | Dutta P.,Central Muga Eri Research and Training Institute | Ghose J.,Central Muga Eri Research and Training Institute
Indian Journal of Traditional Knowledge | Year: 2010

Sericulture is one of the oldest professions the humane developed and practiced. Weaving as a craft also developed independently in ancient China, India, and Thailand as well as in the Middle East, Europe, Africa and in South America. The process of working out new techniques continued for millennia and continues even today. With the change from subsistence farming to the present commercial system and incorporation of mechanical technology have brought in drastic changes in both sericulture and weaving industry. Consequently, people realize the present day techniques adopted for commercial system may be unsustainable in the long run. Currently, scientists are relearning the traditional or indigenous know how and exploring possibilities of using the old practices wherever possible. Further in case of tribal weaving in Northeast India, each region has unique aptitude for the craft of weaving and each tribe has its own traditional style and design. Therefore, introduction of indigenous know how (traditional craft, style, design, etc.) in the field of sericulture and weaving blended with modern techniques can produce low cost quality product with quantity production to sustain present day competition market.

Arunkumar K.P.,DNA Diagnostics Center | Sahu A.K.,Central Muga Eri Research and Training Institute | Mohanty A.R.,Seribiotech Research Laboratory | Awasthi A.K.,Seribiotech Research Laboratory | And 3 more authors.
PLoS ONE | Year: 2012

Background: The Indian golden saturniid silkmoth (Antheraea assama), popularly known as muga silkmoth, is a semi-domesticated silk producing insect confined to a narrow habitat range of the northeastern region of India. Owing to the prevailing socio-political problems, the muga silkworm habitats in the northeastern region have not been accessible hampering the phylogeography studies of this rare silkmoth. Recently, we have been successful in our attempt to collect muga cocoon samples, although to a limited extent, from their natural habitats. Out of 87 microsatellite markers developed previously for A. assama, 13 informative markers were employed to genotype 97 individuals from six populations and analyzed their population structure and genetic variation. Methodology/Principal Findings: We observed highly significant genetic diversity in one of the populations (WWS-1, a population derived from West Garo Hills region of Meghalaya state). Further analysis with and without WWS-1 population revealed that dramatic genetic differentiation (global FST = 0.301) was due to high genetic diversity contributed by WWS-1 population. Analysis of the remaining five populations (excluding WWS-1) showed a marked reduction in the number of alleles at all the employed loci. Structure analysis showed the presence of only two clusters: one formed by WWS-1 population and the other included the remaining five populations, inferring that there is no significant genetic diversity within and between these five populations, and suggesting that these five populations are probably derived from a single population. Patterns of recent population bottlenecks were not evident in any of the six populations studied. Conclusions/Significance: A. assama inhabiting the WWS-1 region revealed very high genetic diversity, and was genetically divergent from the five populations studied. The efforts should be continued to identify and study such populations from this region as well as other muga silkworm habitats. The information generated will be very useful in conservation of dwindling muga culture in Northeast India. © 2012 Arunkumar et al.

Nale J.Y.,University of Leicester | Chutia M.,Central Muga Eri Research and Training Institute | Carr P.,University of Leicester | Hickenbotham P.T.,University of Leicester | Clokie M.R.J.,University of Leicester
Frontiers in Microbiology | Year: 2016

Clostridium difficile infection (CDI) is a global health threat associated with high rates of morbidity and mortality. Conventional antibiotic CDI therapy can result in treatment failure and recurrent infection. C. difficile produces biofilms which contribute to its virulence and impair antimicrobial activity. Some bacteriophages (phages) can penetrate biofilms and thus could be developed to either replace or supplement antibiotics. Here, we determined the impact of a previously optimized 4-phage cocktail on C. difficile ribotype 014/020 biofilms, and additionally as adjunct to vancomycin treatment in Galleria mellonella larva CDI model. The phages were applied before or after biofilm establishment in vitro, and the impact was analyzed according to turbidity, viability counts and topography as observed using scanning electron and confocal microscopy. The infectivity profiles and efficacies of orally administered phages and/or vancomycin were ascertained by monitoring colonization levels and larval survival rates. Phages prevented biofilm formation, and penetrated established biofilms. A single phage application reduced colonization causing extended longevity in the remedial treatment and prevented disease in the prophylaxis group. Multiple phage doses significantly improved the larval remedial regimen, and this treatment is comparable to vancomycin and the combined treatments. Taken together, our data suggest that the phages significantly reduce C. difficile biofilms, and prevent colonization in the G. mellonella model when used alone or in combination with vancomycin. The phages appear to be highly promising therapeutics in the targeted eradication of CDI and the use of these models has revealed that prophylactic use could be a propitious therapeutic option. © 2016 Nale, Chutia, Carr, Hickenbotham and Clokie.

Das R.,Central Muga Eri Research and Training Institute | Das K.,Central Muga Eri Research and Training Institute
Indian Silk | Year: 2010

The spread of muscardine disease in pre-seed muga silkworm crops and the steps taken to manage it are discussed. The disease is transmitted by a sexual structure of the fungus B. bassiana that forms spores on the outer body surface of the diseased muga silkworms after its death, contaminated with muga food plant leaves and rearing appliances. Selection of better quality seeds is one of the most significant steps that needs to be taken to manage the spread of the disease in such silkworm crops. Use of disease free seed is necessary for muga culture and the certified seed produced by different government agencies and NGOs prevents disease transmission. Deep ploughing of the rearing field before and after the rearing of muga silkworm is another steps that helps to eliminate inoculum of the pathogen from the soil.

Gupta A.,ICAR National Bureau of Agricultural Insect Resources | Das A.K.,Gauhati University | Neog K.,Central Muga Eri Research and Training Institute | Verghese A.,ICAR National Bureau of Agricultural Insect Resources
Florida Entomologist | Year: 2016

A gregarious larval parasitoid, Cotesia dictyoplocae (Watanabe) (Hymenoptera: Braconidae: Microgastrinae), is reported for the first time from the host Antheraea assamensis Helfer (Lepidoptera: Saturniidae) from Assam, India. This wasp is a gregarious larval parasitoid and produces on an average 30 cocoons per host larva. Parasitized larvae are easily identified by the aggregation of exposed yellowish white cocoons on the body. Larval parasitism in natural conditions was low. Removal of parasitized larvae by hand is suggested.

PubMed | Zhejiang Sci-Tech University, Central Muga Eri Research and Training Institute and Indian Institute of Technology Kharagpur
Type: | Journal: BioMed research international | Year: 2016

Silk protein sericins, in the recent years, find application in cosmetics and pharmaceuticals and as biomaterials. We investigate the potential of sericin, extracted from both mulberry Bombyx mori and different non-mulberry sources, namely, tropical tasar, Antheraea mylitta; muga, Antheraea assama; and eri, Samia ricini, as growth supplement in serum-free culture medium. Sericin supplemented media containing different concentrations of sericins from the different species are examined for attachment, growth, proliferation, and morphology of fibrosarcoma cells. The optimum sericin supplementation seems to vary with the source of sericins. The results indicate that all the sericins promote the growth of L929 cells in serum-free culture media; however, S. ricini sericin seems to promote better growth of cells amongst other non-mulberry sericins.

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