Cheng S.H.,University of California at Los Angeles |
Anderson F.E.,Southern Illinois University Carbondale |
Bergman A.,Southern Illinois University Carbondale |
Mahardika G.N.,Udayana University |
And 7 more authors.
Hydrobiologia | Year: 2014
The big-fin reef squid, Sepioteuthis cf. lessoniana (Lesson 1930), is an important commodity species within artisanal and near-shore fisheries in the Indian and Indo-Pacific regions. While there has been some genetic and physical evidence that supports the existence of a species complex within S. cf. lessoniana, these studies have been extremely limited in scope geographically. To clarify the extent of cryptic diversity within S. cf. lessoniana, this study examines phylogenetic relationships using mitochondrial genes (cytochrome oxidase c, 16s ribosomal RNA) and nuclear genes (rhodopsin, octopine dehydrogenase) from nearly 400 individuals sampled from throughout the Indian, Indo-Pacific, and Pacific Ocean portions of the range of this species. Phylogenetic analyses using maximum likelihood methods and Bayesian inference identified three distinct lineages with no clear geographic delineations or morphological discriminations. Phylogeographic structure analysis showed high levels of genetic connectivity in the most widespread lineage, lineage C and low levels of connectivity in lineage B. This study provides significant phylogenetic evidence for cryptic lineages within this complex and confirms that cryptic lineages of S. cf. lessoniana occur in sympatry at both small and large spatial scales. Furthermore, it suggests that two closely related co-occurring cryptic lineages have pronounced differences in population structure, implying that underlying differences in ecology and/or life history may facilitate co-occurrence. Further studies are needed to assess the range and extent of cryptic speciation throughout the distribution of this complex. This information is extremely useful as a starting point for future studies exploring the evolution of diversity within Sepioteuthis and can be used to guide fisheries management efforts. © 2013 Springer Science+Business Media Dordrecht.
Rajakumaran P.,Alagappa University |
Vaseeharan B.,Alagappa University |
Jayakumar R.,Central Marine Fisheries Research Institute CMFRI |
Chidambara R.,National Prawn Co
Cytology and Genetics | Year: 2014
Understanding of accurate phylogenetic relationship among Penaeidae shrimp is important for academic and fisheries industry. The Morphometric and Randomly Amplified Polymorphic DNA (RAPD) analysis was used to make the phylogenetic relationsip among 13 Penaeidae shrimp. For morphometric analysis forty variables and total lengths of shrimp were measured for each species, and removed the effect of size variation. The size normalized values obtained was subjected to UPGMA (Unweighted Pair-Group Method with Arithmetic Mean) cluster analysis. For RAPD analysis, the four primers showed reliable differentiation between species, and used correlation coefficient between the DNA banding patterns of 13 Penaeidae species to construct UPGMA dendrogram. Phylogenetic relationship from morphometric and molecular analysis for Penaeidae species found to be congruent. We concluded that as the results from morphometry investigations concur with molecular one, phylogenetic relationship obtained for the studied Penaeidae are considered to be reliable. © 2014, Allerton Press, Inc.
Kannan K.,Central Marine Fisheries Research Institute CMFRI |
John S.,Dakshin Foundation |
Joshi K.K.,Central Marine Fisheries Research Institute CMFRI |
Zacharia P.U.,Central Marine Fisheries Research Institute CMFRI
Marine Biodiversity Records | Year: 2013
Neobythites stefanovi is recorded for the first time from Indian waters in the Gulf of Mannar. Two specimens were landed as by-catch in trawl gear at Tuticorin fisheries harbour, south-east coast of India, in March 2010. This is also the first record of the species outside the Red Sea, Gulf of Oman and Gulf of Aden, in the western Indian Ocean. © Marine Biological Association of the United Kingdom 2013.
Sarangi R.K.,The GEO Group |
Mohammed G.,Central Marine Fisheries Research Institute CMFRI
Indian Journal of Marine Sciences | Year: 2011
Algal bloom has been observed using IRS-P4 (Oceansat-1) OCM data and in situ observations during September 2002 and 2003 around the Kerala coastal and shelf water off Calicut. Algal bloom features have been observed in the total radiance, remote sensing reflectance, chlorophyll and diffuse attenuation coefficient images. In situ observations indicated the dominance of dinoflagellate Noctiluca scintillensis in the 1st, 2nd and 3rd weeks of September 2002. Red colour of water was seen even upto 30-35 kilometer from coastline and this has been got reflected in OCM derived chlorophyll images with he dense algal bloom features with very high chlorophyll concentration (20-50 mg/m3). During September (8, 20, 22, 24 and 30) 2003, high dense algal bloom patches (chl>20 mg/m3) were seen around the Calicut coast in the OCM images and spreads across the Kerala coast in the shelf water up to 20-30 km from coastline. Water colour has been found even green due to toxic micro algae Hornelia marina. Water quality parameters like SST, dissolved oxygen, salinity, pH, productivity and nutrients have been correlated with the bloom phenomenon.
Vikas P.A.,Central Marine Fisheries Research Institute CMFRI |
Sajeshkumar N.K.,Central Marine Fisheries Research Institute CMFRI |
Thomas P.C.,Central Marine Fisheries Research Institute CMFRI |
Chakraborty K.,Central Marine Fisheries Research Institute CMFRI |
Vijayan K.K.,Central Marine Fisheries Research Institute CMFRI
Hydrobiologia | Year: 2012
Autochthonous parthenogentic Artemia populations have been reported from Indian hypersaline habitats since 1950s. Exotic Artemia franciscana was imported and introduced into India as live food for aquaculture since the early eighties. To assess the present status of the Artemia populations and the possibility of invasion by the introduced A. franciscana in Indian Salinas, an extensive study was conducted using conventional and molecular approaches. Morphological and biometric observations, crossbreeding experiments and molecular and phylogenetic analysis using Internally Transcribed Spacer-1 sequence revealed the extensive presence of alien, sexual A. franciscana populations in various hypersaline areas. Individual culture experiments and crossbreeding studies further confirmed the absence of autochthonous parthenogentic Artemia populations. Lack of regional endemism in populations of distant origins was evident, indicating that the invaded populations have naturalized and are in the process of evolution. This forms the first report of invasion by A. franciscana in hypersaline habitats of Indian subcontinent and further studies are required to assess the biological implications of this invasion. © 2011 Springer Science+Business Media B.V.
Madhu K.,Central Marine Fisheries Research Institute CMFRI |
Madhu R.,Central Marine Fisheries Research Institute CMFRI |
Retheesh T.,Central Marine Fisheries Research Institute CMFRI
Aquaculture | Year: 2016
Reproductive behaviour, captive breeding, embryonic, larval development and suitable live feed for Pseudochromis dilectus were found out. Fishes ranging in total length 80-120 mm (male) and 70-80 mm (female) were reared in FRP tank (1000-L) for six months to develop pairs. The pairs laid ball-shaped egg mass (25 to 35 mm diameter) which consisted of 400 to 500 spherical eggs/pair/spawning (n = 18).The size of the individual egg varied between 1743 and 1919 μm and all the eggs were interconnected by fine sticky threads. The egg ball was white or transparent on the first and second day, black on 3rd day and silvery on 4th day of incubation. The hatching rate varied between 91 and 95%, and the peak hatching took place under complete darkness on completion of 96 h incubation. Total length of the newly hatched larvae varied between 5.1 and 5.3 mm with mouth gape 150 to 160 μm. First feeding started at 10 to 12 h after post hatch. The study concluded that Euplotes sp. (0 to 5 dph), enriched rotifer (6-15 dph) and microalgae enriched Diaphanosoma celebensis (16 to 30 dph) can be used as effective feed for high survival of larvae of P. dilectus. On 20 dph the metamorphosis initiated and became juvenile stage at 30 dph (82%). At 45 dph, all the larvae transformed to juvenile and shifted from pelagic to epibenthic in the aquarium with denser body having reddish colouration. Statement of relevance: Pseudochromis dilectus (Family: Pseudochromidae) is a species of keen interest in marine aquarium trade and has distribution in the Western and Central Indian Ocean to Sri Lanka. As its males are colourful than the female, males are selectively exploited from the nature for aquarium trade which is also causing threat to natural population thereby reducing its availability. Hence captive production is a potential solution for reducing pressure on the natural stocks. However no scientific studies were conducted in P. dilectus with an objective to captive breeding. Hence the present study was aimed to generate baseline information on its reproductive behaviour, spawning, egg morphology, embryonic and larval development of P. dilectus and also to find out suitable live feed for higher survivability of larvae, their metamorphosis and juvenile production under captive conditions with a view to develop a reliable captive breeding and rearing techniques for its mass scale production. © 2016 Elsevier B.V.
Gopinath R.,Microbiology and Biochemistry |
Paul Raj R.,Coastal Aquaculture Authority |
George K.C.,Central Marine Fisheries Research Institute CMFRI |
Sanil N.K.,Central Marine Fisheries Research Institute CMFRI
Aquaculture Research | Year: 2012
Fungal contamination of shrimp feeds affects the shelf life leading to substantial economic losses. Ultrastructural changes in Penaeus monodon sub-adults fed three different doses (50, 1000 and 2000ppb) of aflatoxin B 1 (AFB 1) were studied. At the end of 4 and 8 weeks of experiment, the hepatopancreas of shrimps were observed for ultrastructural changes. The prominent ultrastructural changes in hepatopancreas of the shrimps fed 1000 and 2000ppb AFB 1 were rupture of cell membrane and microvillus border, damage and swelling of mitochondria, fragmentation of endoplasmic reticulum, nuclear vacuolation, chromatin condensation and autophagy. This study helps to decipher the ultrastructural changes and relate the effects on biochemical, pathological, immunological and histological architecture of the shrimps fed AFB 1-incorporated feed. The observed ultrastructural changes could serve as indicators in shrimps exposed to toxicants. © 2011 Blackwell Publishing Ltd.
Sunith Shine S.R.,Central Marine Fisheries Research Institute CMFRI |
Feroz Khan M.,Scott Christian College Autonomous |
Godwin Wesley S.,Scott Christian College Autonomous
Marine Pollution Bulletin | Year: 2013
Polonium-210 activity concentration was analysed in the whole body tissue of periwinkle Littorina undulata collected from intertidal rocky shore along Kudankulam coast. We carried out the study for a period of 12months (2011-2012) focusing on three seasons. 210Po was found non-uniformly distributed among the periwinkles depending on the allometry. The 210Po accumulation showed a significant difference between seasons (p<0.05). Smaller sized winkles registered higher activity of 210Po compared to larger ones (p<0.05). The overall activity range of 210Po varied from 13.5 to 58.9Bq/kg (wet). The activity of 210Po was also quantified in seawater and intertidal sediments to calculate the biological concentration factor (BCF) and radiation dose rate. The dose rate to the winkles was performed using ERICA Assessment Tool and it was within the prescribed limit. The intake of 210Po through periwinkles delivered an effective dose in the range of 2.2-9.6μSv/y to human beings. © 2013 Elsevier Ltd.
Akhilesh K.V.,Central Marine Fisheries Research Institute CMFRI |
Bineesh K.K.,Central Marine Fisheries Research Institute CMFRI |
Zootaxa | Year: 2012
A new serranid fish, Liopropoma randalli n. sp. is described from four specimens collected from the Indian Ocean off southwestern India and eastern Indonesia. It differs from all other species in the genus in its striking color pattern, a broad black band from behind the eye to the caudal peduncle, semicircular dark-brown to black spots that cover the pink to reddish body, and a combination of the following characters: 46 to 49 lateral line scales; 1-2 (4 as rudiments) + 12-13 (4-5 as rudiments) gill rakers on the first arch (total 17-19); longest dorsal soft ray 2.1-2.3 in head length; 1st anal-fin spine 10.4-12.2 in head length; 2nd anal-fin spine 4.4-4.9 in head length; pelvic fin relatively short, 5.1-5.7 in SL; and body depth 3.2-3.6 in SL. Copyright © 2012 Magnolia Press.
Anju A.,Central Marine Fisheries Research Institute CMFRI |
Jeswin J.,Central Marine Fisheries Research Institute CMFRI |
Thomas P.C.,Central Marine Fisheries Research Institute CMFRI |
Vijayan K.K.,Central Marine Fisheries Research Institute CMFRI
Fish and Shellfish Immunology | Year: 2013
F-type lectin is an important type of pattern recognition receptor that can recognize and bind carbohydrate moieties on the surface of potential pathogens through its carbohydrate recognition domains (CRDs). This paper reports the cloning of an F-type lectin (designated as pfF-type lectin) from the pearl oyster (Pinctada fucata) using rapid amplification of cDNA ends (RACE) PCR. The full-length cDNA of this pfF-type lectin contains an open reading frame (ORF) of 588bp coding for196 amino acids. A signal peptide at the N-terminus of the deduced polypeptide was predicted by the signal P program and the cleavage site is located between the positions of Gly19and Tyr20. Conserved domain search at NCBI revealed the pfF-type lectin domain extends from Lys55to Val192. Semi-quantitative analysis in adult tissues showed that the pfF-type lectin mRNA was abundantly expressed in haemocytes and gill and rarely expressed in other tissues tested. After challenge with lipopolysaccharide (LPS), expression of pfF-type lectin mRNA in haemocytes was increased, reaching the highest level at 4h, then dropping to basal levels at 36h. These results suggest that F-type lectin play a critical role in the innate immune system of the pearl oyster P.fucata. © 2013.