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Luzon, Philippines

Central Luzon State University is a state university located in a 658-hectare campus in Science City of Muñoz, Nueva Ecija, Philippines. The University is the lead agency of the Muñoz Science Community and the seat of the Regional Research and Development Center in Central Luzon. To date, CLSU is one of the premiere institutions for agriculture in the Philippines and in Southeast Asia known for its research in aquaculture, ruminants, crops, orchard, and water management.CLSU is first comprehensive state university to undergo institutional accreditation Wikipedia.

Quiazon K.M.A.,University of Tokyo | Quiazon K.M.A.,Central Luzon State University | Yoshinaga T.,University of Tokyo | Ogawa K.,University of Tokyo
Parasitology International | Year: 2011

Human anisakiasis is caused by the consumption of raw, marinated or undercooked fish and squid infected with nematodes of the genus Anisakis Dujardin, 1845. In view of food safety, this study was carried out to examine the distribution of Anisakis species in marine fishes within Japanese waters. Seven fish species from six localities were collected and examined for Anisakis infection. Morphological and molecular (ITS region and mtDNA cox2 gene) characterization revealed the presence of two, among the three sibling species of Anisakis simplex, viz. A. simplex sensu stricto (s.s.) and A. pegreffii. Distribution data were collated with the results from the previous researches to better understand Anisakis distribution in Japanese waters. Distributions of Anisakis species were found to be locality-specific rather than host-specific, particularly between the two major species, A. simplex s.s. and A. pegreffii. Anisakis simplex s.s. is mainly found in fishes from northern Japan to Pacific sides, whereas A. pegreffii is in fishes from the Sea of Japan to East China Sea sides. © 2011 Elsevier Ireland Ltd.

Quiazon K.M.A.,University of Tokyo | Quiazon K.M.A.,Central Luzon State University | Zenke K.,University of Tokyo | Yoshinaga T.,University of Tokyo
Molecular and Biochemical Parasitology | Year: 2013

It remains unclear whether allergens are the same among the sibling species of Anisakis simplex sensu lato. This study was carried out to compare the amino acid sequences of three major (Ani s 1, 2 and 12) and one minor (Ani s 9) Anisakis allergens between A. simplex s.s. and Anisakis pegreffii. We found 2 (out of 163), 1 (out of 869), and 29 (out of 266) amino acid variable sites for Ani s 1, 2, and 12, respectively. However, as both intra- and inter-species variations were present at the same amino acid positions, no amino acid variations clearly distinguished the two sibling species. IgE-binding epitopes (Ani s 1) and a binding motif of human leukocyte antigen (Ani s 2 and 9) demonstrated by previous studies were conserved. The similarities of the amino acid sequences of the allergens indicate possible similar allergy-associated health risks in humans infected with or accidentally ingesting either Anisakis species. © 2013 Elsevier B.V. All rights reserved.

Cetaceans are definitive hosts of anisakid nematodes known to cause human anisakidosis. Despite the reported strandings of different cetaceans in the Philippines, studies on anisakids from these definitive hosts are limited. Here, the morphologically and molecularly identified anisakid species, specifically those of the genus Anisakis Dujardin, 1845 in stranded Pygmy Sperm Whale Kogia breviceps Blainville, 1838 in the west Pacific region off Philippine waters are presented. Morphological data using SEM and LM revealed multi-infections with different Anisakis species belonging to Anisakis type I and type II groups. Molecularly, PCR-RFLP on the ITS rDNA and sequence data analyses of both ITS rDNA and mtDNA cox2 regions identified those from Anisakis type I group as A. typica (Diesing, 1860), whereas those from type II group as A. brevispiculata Dollfus, 1968, and A. paggiae Mattiucci et al. (Syst Parasitol 61:157–171, 2005). This is the first record of Anisakis infection from this host stranded in the west Pacific region off the coast of Philippine waters and new geographical record for A. paggiae. © 2016 Springer-Verlag Berlin Heidelberg

Quiazon K.M.A.,University of Tokyo | Quiazon K.M.A.,Central Luzon State University | Yoshinaga T.,University of Tokyo | Ogawa K.,University of Tokyo
Parasitology International | Year: 2011

The third-stage larvae of Anisakis simplex sensu lato (s.l.) are found in many marine fishes. To ensure food safety, it is important to determine whether these larvae are present in the body muscle of commercial fish species. However, there is little information regarding the tissue specificity of Anisakis and two of its sibling species, A. simplex sensu stricto (s.s.) and Anisakis pegreffii, that are common in marine fish in Japanese waters. We orally challenged rainbow trout (Oncorhynchus mykiss (Walbaum)), and olive flounder (Paralichthys olivaceus (Temminck and Schlegel)) with L3 larvae of these two sibling species and monitored infection for 5. weeks. In rainbow trout, A. simplex s.s., but not A. pegreffii larvae, migrated into the body muscle. A small number of freely moving A. pegreffii larvae were recovered within the body cavity. In olive flounder, A. simplex s.s. larvae were found in both the body cavity and body muscle. A. pegreffii larvae were found only in the body cavity and primarily encapsulated in lumps. Our results indicate that there are differences in the sites of infection and host specificity between the two sibling species of A. simplex s.l. © 2010 Elsevier Ireland Ltd.

Alvindia D.G.,Central Luzon State University
Journal of General Plant Pathology | Year: 2013

The efficacy of Trichoderma harzianum strain DGA01, sodium carbonate (SC), sodium bicarbonate (SBC) and sodium hypochlorite (SH) applied alone or in various combinations was evaluated in vitro against the most important postharvest pathogens of banana such as Lasiodiplodia theobromae, Thielaviopsis paradoxa, Colletotrichum musae, and Fusarium verticillioides. Trichoderma harzianum DGA01 was compatible with salts at a concentration of 1 % (w/v) as manifested by normal mycelial growth. The efficacy of the fungal antagonist in vitro was enhanced by 10. 16-13. 06 % in controlling mycelial growth of crown rot pathogens with the addition of salts. After a postharvest dip of fruit for 30 min in 106 conidia of DGA01/mL of 1 % SBC, the incidence of crown rot was reduced by 92 %, but DGA01 combined with SC or SH had no additive effect in controlling crown rot. Thus, DGA01 and SBC was the best combination for crown rot control, with an efficacy similar to synthetic fungicides, and maintained the overall quality of banana even at conditions favourable for the pathogens (22-25 °C, 90-95 % relative humidity). © 2013 The Phytopathological Society of Japan and Springer Japan.

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