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Arulprakash K.,University of Madras | Murugan R.,University of Madras | Ponrasu T.,Central Leather Research Institute Council of Scientific and Industrial Research | Iyappan K.,SSN College of Engineering | And 2 more authors.
Clinical and Experimental Dermatology | Year: 2012

Background. Wound healing occurs as a fundamental response to tissue injury. Several natural products have been shown to accelerate the healing process. Aim. To observe the efficacy of topical administration of an ethanolic extract of Ageratum conyzoides on cutaneous wound healing in rats. Methods. An ethanolic extract of A. conyzoides was prepared, and its wound-healing efficacy on rats was studied. An open excision wound was made on the back of each rat, and 200 μL (40 mg/kg body weight) of the A. conyzoides extract was applied topically once daily to the treated wounds. The control wounds were treated with 200 μL of 50% ethanol. The wound tissues formed were removed at 4, 8 and 12 days after wounding, and biochemical parameters such as DNA, total protein, total collagen, hexosamine and uronic acid were estimated. The extent of epithelialization and the tensile strength of the wounded tissues were also measured. Results. The A. conyzoides extract increased cellular proliferation and collagen synthesis. Wounds treated with the extract were found to heal much faster, based on the improved rates of epithelialization and wound contraction, and on the histopathological results. A 40% increase in the tensile strength of the treated tissue was seen. Conclusions. Topical application of A. conyzoides accelerates the rate of wound healing. CED © 2012 British Association of Dermatologists. Source


Balaji S.,Central Leather Research Institute Council of Scientific and Industrial Research Adyar | Kumar R.,Central Leather Research Institute Council of Scientific and Industrial Research Adyar | Sripriya R.,Central Leather Research Institute Council of Scientific and Industrial Research Adyar | Rao U.,Central Leather Research Institute Council of Scientific and Industrial Research Adyar | And 4 more authors.
Polymers for Advanced Technologies | Year: 2012

Fabrication of keratin-collagen (KC) 3D scaffold with improved thermal denaturation rate is reported. In vitro application of (KC) scaffold stimulates basic extra cellular matrix constituents. KC Scaffold considerably reduced undesirable properties of both collagen and keratin while collagen incorporation reduces the fragility with increases of strength and flexibility in the scaffold. In addition to this, the scaffold showed homogenous well-interconnected pores in the range of 10-100μm when observed in scanning electron microscope. Usage of keratin in KC scaffold offers increased biodegradation rate and higher denaturation rate in addition to its rapid cell growth with normal morphology ultimately reaching cell population of 3.9-9.7 million per cm 3 after 48hr in KC scaffold. Circular dichroism (CD) and Fourier transform spectroscopy (FT-IR) of KC showed presence of helical structure of collagen and ß-turns of keratin confirming retention of native structures of both the proteins KC scaffold showed good swelling behavior and water uptake. Our study strongly supports the superidity of KC scaffold over the collagen or keratin when they are independently used for tissue engineering applications. © 2011 John Wiley & Sons, Ltd. Source


Alliraja C.,Central Leather Research Institute Council of Scientific and Industrial Research Adyar | Rao J.R.,Central Leather Research Institute Council of Scientific and Industrial Research Adyar | Thanikaivelan P.,Central Leather Research Institute Council of Scientific and Industrial Research Adyar
RSC Advances | Year: 2015

We describe a green and sustainable approach to cross-link collagen fibers in a non-aqueous green medium using oleic acid coated iron oxide nanoparticles. The magnetic nanoparticles coated with oleic acid (OA) were prepared by a simple co-precipitation technique and characterized using X-ray diffraction, UV-Vis-NIR spectroscopy, vibrating sample magnetometer, scanning and transmission electron microscopy. The OA coated nanoparticles, with an average particle size of 5 nm and saturation magnetization of 23.5 emu g-1, were well dispersed in heptane and interacted with the collagen fibers derived from skin trimming wastes. The derived thermo-stable magnetic collagen fibers were further utilized for oil removal applications. We demonstrate that the heptane reaction medium helps in achieving absorption of 8.2 g of used motor oil per g of magnetic collagen fibers with magnetic tracking ability. The approach showcases a simple synthetic protocol for preparing magnetic collagen fibers for possible applications in leather making and environmental protection. © 2015 The Royal Society of Chemistry. Source

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