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Arnaudova-Matey A.,University of Forestry | Yankovska T.,Central Laboratory of Veterinary Control and Ecology | Kirilova T.,Central Laboratory of Veterinary Control and Ecology | Todorova K.,Bulgarian Academy of Science | And 4 more authors.
Bulgarian Journal of Agricultural Science | Year: 2013

35-day tests with broiler chickens treated with Bulgarian iron methionate administered through the food compared to the iron sulphate (heptahydrate) in doses of 60 ppm and 300 ppm were conducted. The test involved 55 broiler chickens aged 10 days, divided into 5 groups of 11 chickens. The tests started on May 21st, 2012 and continued 35 days. The basic mixed feed was prepared by using a recipe for growing broiler chickens and an average content of 85.6 ± 2.4 mg Fe/kg. The appetite, health status (clinical one) and individual weight of the chickens were controlled. On the 15th day samples of the liver from three euthanized chickens of each group were taken for histological and chemical studies. On the 35th day four more chickens of each group were subjected to the same studies. The liver samples intended for chemical analysis were frozen at -18°C and after 22 days were thawed out and tested for iron content by optical emission spectrophotometer ICP-OES 715-S. Samples of the cloacal content were taken from the chickens euthanized on the 15th and 35th day. They were also frozen and then thawed out, dried and analysed for iron content by using atomic absorption spectrophotometer equipped with graphic cuvette, model Spectra AA 800. The statistical results were processed by three different methods - parametric (Anova one-way), non-parametric (Mann-Whitney U-test) method and by using the tables of Student-Fisher. During the test period no clinical symptoms and signs of disease or mortality were found in all treated chickens; there were no pathomorphological changes in the liver of the chickens. In general, the utilisation was more favourable for the iron methionate compared to the iron sulphate. It was better expressed in the low concentration (60 ppm) - a steady growth, trend for better deposition in the liver and significantly smaller amount (up to two times) of iron in the cloacal content (beneficial for the environment). The iron deposited in the liver of the treated chickens was from 40 to 60% more than that in the control ones.

Stoev G.,Central Laboratory of Veterinary Control and Ecology | Xuan Y.,Thermo Fisher Scientific | Peycheva M.,Central Laboratory of Veterinary Control and Ecology | Scigelova M.,Thermo Fisher Scientific
Talanta | Year: 2012

Applications of high resolution mass spectrometry (HRMS) in food safety and residue analysis have increased remarkably over the last few years. The high resolution detection of ions reportedly enhances the assay selectivity but quantitative assessment of HRMS contribution to the assay selectivity has not yet been undertaken. We devised a method to assess the impact of instrument resolution on the probability that a spectral assignment to a given compound was made in error. The method allows for evaluating the quality of a spectral assignment based on resolution and the number of fragmentation stages. It thus provides a firm basis for comparing analytical methods performed on very different mass spectrometric instrumental platforms as well as in the context of the current regulatory framework. © 2012 Elsevier B.V. All rights reserved.

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