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Hu Z.,Chinese Academy of Agricultural Sciences | Jiang Q.,Chinese Academy of Agricultural Sciences | Ni Z.,Chinese Academy of Agricultural Sciences | Chen R.,Central Laboratory of Tianjin Academy of Agricultural science | And 2 more authors.
Journal of Integrative Plant Biology | Year: 2013

Plant microRNAs (miRNAs) regulate gene expression mainly by guiding cleavage of target mRNAs. In this study, a degradome library constructed from different soybean (Glycine max (L.) Merr.) tissues was deep-sequenced. 428 potential targets of small interfering RNAs and 25 novel miRNA families were identified. A total of 211 potential miRNA targets, including 174 conserved miRNA targets and 37 soybean-specific miRNA targets, were identified. Among them, 121 targets were first discovered in soybean. The signature distribution of soybean primary miRNAs (pri-miRNAs) showed that most pri-miRNAs had the characteristic pattern of Dicer processing. The biogenesis of TAS3 small interfering RNAs (siRNAs) was conserved in soybean, and nine Auxin Response Factors were identified as TAS3 siRNA targets. Twenty-three miRNA targets produced secondary small interfering RNAs (siRNAs) in soybean. These targets were guided by five miRNAs: gma-miR393, gma-miR1508, gma-miR1510, gma-miR1514, and novel-11. Multiple targets of these secondary siRNAs were detected. These 23 miRNA targets may be the putative novel TAS genes in soybean. Global identification of miRNA targets and potential novel TAS genes will contribute to research on the functions of miRNAs in soybean. © 2012 Institute of Botany, Chinese Academy of Sciences. Source


Li N.,Central Laboratory of Tianjin Academy of Agricultural science | Li H.,Central Laboratory of Tianjin Academy of Agricultural science | Shao H.,Central Laboratory of Tianjin Academy of Agricultural science | Liu L.,Central Laboratory of Tianjin Academy of Agricultural science | And 2 more authors.
Chinese Journal of Chromatography (Se Pu) | Year: 2011

An ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/ MS) method was developed for the determination of the fifteen sulfonylurea herbicides in ginseng. The pesticides were extracted with acetonitrile, cleaned-up by an ENVI-Carbon vsolid phase extraction cartridge, eluted with 1% formic acid in methanol-dichlormethane (20: 80, v/v), separated with UPLC and detected with MS/MS in multiple reaction monitoring (MRM) mode via positive electrospray ionization (ESI +). The method was validated at three fortification levels in ginseng. The validation results were as follows. The standard calibration curves for the fifteen sulfonylurea herbicides all showed linear over the range of 2 -100 μg/L with the correlation coefficients between 0. 996 and 0. 999. The average recoveries of the fifteen sulfonylurea herbicides at the three fortification levels of 5, 25 and 50 μg/kg were between 84. 9% and 104. 3% with the relative standard deviations (RSDs) between 2. 4% and 11. 9%. The limit of quantification (LOQ) was defined as the lowest concentration that could be measured with acceptable precision and accuracy. The LOQs of all the fifteen sulfonylurea herbicides in ginseng were 5 μg/kg. It was indicated that this method is easier, more sensitive and has a better purification effect. Thex^eftsitivity, accuracy and precision of the method were all acceptable. So, this method can be further applied to investigating the contamination status of traditional Chinese medicine by the sulfonylurea herbicides. Source

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