Central Laboratory of Shaanxi Provincial Peoples Hospital

Fengcheng, China

Central Laboratory of Shaanxi Provincial Peoples Hospital

Fengcheng, China
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Shi W.,Chinese National Institute for Viral Disease Control and Prevention | Xu Y.,Chinese National Institute for Viral Disease Control and Prevention | Liu Y.,Central Laboratory of Shaanxi Provincial Peoples Hospital | Guan L.-Y.,Chinese National Institute for Viral Disease Control and Prevention | And 4 more authors.
Journal of Xi'an Jiaotong University (Medical Sciences) | Year: 2017

Objective: To analyze the epidemiological characteristics of other-infectious diarrhea (OID) in Shaanxi Province from 2006 to 2015 to further clarify the prevention and control strategies. Methods: We collected data from the Infectious Disease Report Information System in China for Disease Prevention and Control and made a statistical description of the epidemiological characteristics of incidence distribution in Shaanxi from 2006 to 2015. Results: Totally 207 437 cases of other-infectious diarrhea were reported in Shaanxi Province during 2006-2015, with the average yearly incidence rate of 52.43/105. The incidence number was higher in males (126 673 cases) than in females (80 764 cases), with male-female ratio of 1.57:1. The diseases were most serious in age groups 0- and 1-4 years, accounting for 72.48%. The top-three careers of reported cases were scattered children (74.88%), farmers (10.75%) and students (5.75%), respectively. The cities with the highest incidence in Shaanxi Province were Baoji, Yan'an and Ankang. Two high-incidence seasons were summer (July to August) and winter (November to December). The main pathogen of viral diarrhea was rotavirus, but laboratory-confirmed proportion was low (4.60%). Conclusion: Other-infectious diarrhea, especially infection caused by rotavirus in autumn and winter, mainly occurred in children younger than 5 years old. Etiological diagnosis should be strengthened because of the poor laboratory diagnosis reporting rate. © 2017, Editorial Board of Journal of Xi'an Jiaotong University (Medical Sciences). All right reserved.


Xie X.,Northwest University, China | Xie X.,Institute of Integrated Medical Information | Wang C.,The Tumor Research Institute of Jilin Province | Xie Y.,Northwest University, China | And 7 more authors.
Journal of Immunological Methods | Year: 2013

CD306, also known as soluble leukocyte-associated immunoglobulin-like receptor-2 (LAIR-2), is a member of an immunoglobulin superfamily with the shared characteristic of an immunoglobulin-like C2-type domain. CD306 is speculated to be secretory and has 84% similarity with the extracellular domain of CD305, which binds to the same ligands as CD306. However, data on its distribution are absent due to the lack of an efficient method to detect it. In this study, we successfully cloned the cDNA of CD306 from the peripheral blood mononuclear cells (PBMCs) of patients with hemorrhagic fever with renal syndrome. The fusion proteins were expressed and purified, and three strains of monoclonal antibodies (mAbs) against CD306 were prepared and characterized. The sandwich ELISA for detecting CD306 was established and optimized with sensitivity up to 15. pg/ml, and the assay showed high specificity for the detection of CD306. With this method, a right skewed frequency distribution of CD306 in the sera of healthy subjects was determined. The concentrations of CD306 in sera and urine were detected in patients with different diseases. Aberrantly high levels of CD306 were found in the sera of pregnant women and patients with inflammation and rheumatic heart disease and in the urine of pregnant women. Meanwhile, there was a positive correlation between CD306 and soluble CD305 expression and secretion levels in sera and urine samples from patients, and both proteins inhibited CD305-mediated immunosuppressive functions. Our results demonstrate that CD306 represents a potentially useful predictor for disease diagnosis and that the method developed has potential for clinical application. © 2013 Elsevier B.V.


Li H.J.,Xi'an Jiaotong University | Guo C.Y.,Central Laboratory of Shaanxi Provincial Peoples Hospital | Sun J.Y.,Central Laboratory of Shaanxi Provincial Peoples Hospital | Sun L.J.,Central Laboratory of Shaanxi Provincial Peoples Hospital | And 4 more authors.
Genetics and Molecular Research | Year: 2014

The nested polymerase chain reaction (PCR) method was used for the amplification of the influenza A H1N1 virus hemagglutinin monoclonal antibody light-chain and heavy-chain genes. Sequence analysis of the obtained genes was then used to identify common cloning methods of the mouse immunoglobulin-kappa (Igκ) light-chain and heavy-chain variable gene regions. Twenty-two pairs of amplification primers for the mouse Igκ light-chain and heavy-chain variable gene regions were designed, and 6 mouse anti-human H1N1 influenza virus hemagglutinin monoclonal antibody light-chain and heavy-chain variable gene regions were cloned and sequenced. Comparative analysis was conducted between our results and the mouse Ig sequences published in the National Center of Biotechnology Information (NCBI). The nested PCR method effectively avoided cloning the pseudogenes of the monoclonal antibody, and the amino acid sequence obtained was consistent with the characteristics of the mouse Ig variable region. A general method of cloning the mouse Ig light-chain and heavy-chain variable gene regions was established, which provides a basis for further cloning of mouse monoclonal antibody variable gene regions. This study also provides data for further studies of H1N1 influenza virus hemagglutinin antibody binding sites. © FUNPEC-RP.


PubMed | Central Laboratory of Shaanxi Provincial Peoples Hospital and Xi'an Jiaotong University
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2014

The nested polymerase chain reaction (PCR) method was used for the amplification of the influenza A H1N1 virus hemagglutinin monoclonal antibody light-chain and heavy-chain genes. Sequence analysis of the obtained genes was then used to identify common cloning methods of the mouse immunoglobulin-kappa (Ig) light-chain and heavy-chain variable gene regions. Twenty-two pairs of amplification primers for the mouse Ig light-chain and heavy-chain variable gene regions were designed, and 6 mouse anti-human H1N1 influenza virus hemagglutinin monoclonal antibody light-chain and heavy-chain variable gene regions were cloned and sequenced. Comparative analysis was conducted between our results and the mouse Ig sequences published in the National Center of Biotechnology Information (NCBI). The nested PCR method effectively avoided cloning the pseudogenes of the monoclonal antibody, and the amino acid sequence obtained was consistent with the characteristics of the mouse Ig variable region. A general method of cloning the mouse Ig light-chain and heavy-chain variable gene regions was established, which provides a basis for further cloning of mouse monoclonal antibody variable gene regions. This study also provides data for further studies of H1N1 influenza virus hemagglutinin antibody binding sites.

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