Central Laboratory of Navy General Hospital

Beijing, China

Central Laboratory of Navy General Hospital

Beijing, China

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Jiang X.,Henan Normal University | Jiang X.,Central Laboratory of Navy General Hospital | Zhang Y.,Central Laboratory of Navy General Hospital | Yan H.,South China University of Technology | And 3 more authors.
Journal of Infection in Developing Countries | Year: 2014

Introduction: Rapid dissemination of plasmid-mediated quinolone resistance (PMQR) has been reported in clinical isolates.Methodology: A total of 149 clinical isolates of Enterobacteriaceae were collected in Beijing and screened for PMQR genesusingpolymerase chain reaction (PCR). Real-time quantitative PCR was used to study the expression of qnrS.Results: The rates of qnr and aac(6’)-Ib-cr genes were 7.4% and 8.1%, respectively. The higher basal expression of qnrS was observed in transconjugant strains, which had higher minimum inhibitory concentrations (MICs) of quinolones. Furthermore, qnrS expression levels increased in all three isolates when a quinolone was present.Conclusions: Our data suggest that the level of qnrS expression was associated with quinolone resistance. © 2014 Jiang et al.


Jiang X.,South China University of Technology | Jiang X.,Central Laboratory of Navy General Hospital | Jiang X.,Tech Lab Group | Zhou L.,Central Laboratory of Navy General Hospital | And 12 more authors.
Microbiology and Immunology | Year: 2012

The expression of efflux pump gene lde in ciprofloxacin resistant (CipR) and susceptible strains of Listeria monocytogenes collected from retail food samples was investigated. For two CipR strains, the MICs of ciprofloxacin decreased four- to eightfold in the presence of reserpine; however, no significant alterations were observed with naturally sensitive isolates. Overexpression of the lde gene induced by ciprofloxacin was observed in two resistant isolates. The present findings indicate that expression of lde and the MICs of ciprofloxacin are well correlated with the presence and absence of reserpine, suggesting that Lde might be involved in ciprofloxacin resistance of L. monocytogenes. © 2012 The Societies and Wiley Publishing Asia Pty Ltd.


Jiang X.,Henan Normal University | Yu T.,Xinxiang University | Liang Y.,Xinxiang University | Ji S.,Henan Normal University | And 3 more authors.
International Journal of Food Microbiology | Year: 2016

In this study, efflux pump-mediated benzalkonium chloride (BC) resistance, including plasmid-encoded (Qac protein family and BcrABC) and chromosome-borne efflux pumps, was investigated in Listeria monocytogenes from retail food in China. Among the 59 L. monocytogenes strains, 13 (22.0%) strains were resistant to BC. The PCR results showed that bcrABC was harbored by 2 of 13 BC resistant strains. However, none of the qac genes were detected among the 59 strains. The bcrABC was absent in both of the plasmid cured strains, indicating that this BC resistance determinant was plasmid-encoded in the two bcrABC-positive strains. In the presence of reserpine, most of the bcrABC-negative strains had decreases in the MICs of BC, suggesting the existence of other efflux pumps and their role in BC resistance. After exposed to reserpine, the reduction in BC MICs was observed in the two cured strains, indicating that efflux pumps located on chromosome was also involved in BC resistance. Our findings suggest that food products may act as reservoirs for BC resistant isolates of L. monocytogenes and plasmid- and chromosome-encoded efflux pumps could mediate the BC resistance of L. monocytogenes, which is especially relevant to the adaption of this organism in food-related environments with frequent BC use. © 2015.


PubMed | Henan Normal University, Central Laboratory of Navy General Hospital and Xinxiang University
Type: | Journal: International journal of food microbiology | Year: 2015

In this study, efflux pump-mediated benzalkonium chloride (BC) resistance, including plasmid-encoded (Qac protein family and BcrABC) and chromosome-borne efflux pumps, was investigated in Listeria monocytogenes from retail food in China. Among the 59 L. monocytogenes strains, 13 (22.0%) strains were resistant to BC. The PCR results showed that bcrABC was harbored by 2 of 13 BC resistant strains. However, none of the qac genes were detected among the 59 strains. The bcrABC was absent in both of the plasmid cured strains, indicating that this BC resistance determinant was plasmid-encoded in the two bcrABC-positive strains. In the presence of reserpine, most of the bcrABC-negative strains had decreases in the MICs of BC, suggesting the existence of other efflux pumps and their role in BC resistance. After exposed to reserpine, the reduction in BC MICs was observed in the two cured strains, indicating that efflux pumps located on chromosome was also involved in BC resistance. Our findings suggest that food products may act as reservoirs for BC resistant isolates of L. monocytogenes and plasmid- and chromosome-encoded efflux pumps could mediate the BC resistance of L. monocytogenes, which is especially relevant to the adaption of this organism in food-related environments with frequent BC use.


Zhou L.-J.,Central Laboratory of Navy General Hospital | Huang Q.-X.,South China Agricultural University | Lei H.-T.,South China Agricultural University | Wang Y.-X.,Central Laboratory of Navy General Hospital | And 4 more authors.
Gaodeng Xuexiao Huaxue Xuebao/Chemical Journal of Chinese Universities | Year: 2012

The total RNA was extracted from a hybridoma cell line(12C2) secreting monoclonal antibodies(MAb) against O, O-diethyl phosphorothioate pesticides(DPPs) and reverse-transcribed into cDNA by RT-PCR. The scFv was amplified by gene splicing via overlap extension PCR(SOE-PCR) with the previously designed degenerate primers with a DNA linker encoding(G1y4Ser)3. Then the scFv fragment was cloned into the phagemid p3MH and then the phagemid was transformed into the competent Escherichia coli XL1-Blue. With the rescue of helper phage VCSM13, a phage-display scFv was constructed and then characterized by phage-ELISA. Furthermore, a soluble scFv was gained by wiping off the gene III on p3MH then induced by IPTG. After the detection of SDS-PAGE, Western-Blot and ELISA, characteristics of scFv were compared with those of parental MAb. With a molecular weight of 27000, the DPPs scFv owns a broader specificity and a higher sensitivity than MAb. The IC50 and LOD to detected DPPs enhanced 1.3-3.5 times of parental MAb. This research will provide a new broad-specific and high-sensitive recognition molecule to the detection for DPPs.


Jiang X.,South China University of Technology | Jiang X.,Central Laboratory of Navy General Hospital | Wang D.,Central Laboratory of Navy General Hospital | Wang Y.,Central Laboratory of Navy General Hospital | And 3 more authors.
World Journal of Microbiology and Biotechnology | Year: 2012

The aim of this study was to investigate the prevalence, antimicrobial susceptibility and resistant determinants of Elizabethkingia meningoseptica in a Beijing hospital. Four hundred and eighty-seven samples from medical devices, hospital surfaces and medical staff hands were collected. In total, 26 E. meningoseptica isolates were obtained. The sinks, faucets, and drains accounted for more than half of the total number of isolates recovered. Antimicrobial susceptibility testing revealed that 24 isolates were resistant to one or more antibiotics. All strains were susceptible to piperacillin/tazobactam and vancomycin. Although the trimethoprim/sulfamethoxazole has previously been shown to exhibit good activity against E. meningoseptica, in our study 15 strains were resistant to it. We detected trimethoprim/sulfamethoxazole resistance determinants using PCR; six isolates possessed the sulI gene and four possessed the sulII gene, whilst the dfrA12 gene was detected in only one of them. Pulsed-field gel electrophoresis (PFGE) analysis showed 9 distinct types and one dominant pattern with 12 strains was found. Our data indicate that antimicrobial resistant E. meningoseptica strains exist in the hospital environment and susceptibility testing revealed that vancomycin and piperacillin/tazobactam was the most effective antibiotics. These results have practical significance for treatment of E. meningoseptica infection. © 2012 Springer Science+Business Media B.V.

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