Entity

Time filter

Source Type


Aydin O.,Clinical Biochemistry | Ellidag H.Y.,Central Laboratories of Antalya Education and Research Hospital of Ministry of Health | Ay N.,Clinical Biochemistry | Yalclnkaya S.,Urology Clinic of Antalya Education and Research Hospital of Ministry of Health | Yilmaz N.,Central Laboratories of Antalya Education and Research Hospital of Ministry of Health
Journal of Medical Biochemistry | Year: 2015

Background: Oxidative stress may be involved in the pathogenesis of every human disease. To understand its possible role in benign prostatic hyperplasia (BPH), we measured the overall oxidative status of patients with BPH and the serum activity of the high density lipoprotein (HDL)-related antioxidant enzymes paraoxonase 1 (PON1) and arylesterase (ARE). Methods: Fifty-six urology outpatient clinic patients with BPH (mean age 64±8.6 years) were prospectively included in the study. Forty volunteer healthy controls from the laboratory staff (mean age 62±10 years) were enrolled for comparison. Serum total antioxidant status (TAS), total oxidant status (TOS), PON1, ARE, and HDL levels were measured by commercially available, ready-to-use kits. Results: Serum TAS and HDL levels were significantly lower in the BPH group than in the control group (P=0.004 and P=0.02, respectively). No significant between-group differences were observed for TOS levels or PON1 and ARE enzyme activities (P=0.30, P=0.89, and P=0.74, respectively). In the BPH group, the calculated parameters PON1/HDL and ARE/HDL were significantly higher (P=0.02 and P=0.04, respectively). Conclusions: Our findings agree with the previous reports of impaired oxidant/antioxidant balance in BPH patients. The activities of HDL-related enzymes between groups with significantly different HDL levels may be deceptive; adjusted values may help to reach more accurate conclusions.


Ellidag H.Y.,Central Laboratories of Antalya Education and Research Hospital of Ministry of Health | Aydin O.,Maternaty and Childrens Hospital | Eren E.,Ataturk Hospital | Yilmaz N.,Central Laboratories of Antalya Education and Research Hospital of Ministry of Health | Ergin M.,Ankara Ataturk Education and Research Hospital
Asian Pacific Journal of Cancer Prevention | Year: 2014

Background: Multiple myeloma (MM) is a haematological cancer characterized by clonal proliferation of plasma cells.The aim of this study was to investigate the activity of serum paraoxonase-1 (PON1) and arylesterase (ARE) in multiple myeloma with and without free light chain excretion(FLCe-MM and NFLCe-MM); as well as to investigate possible alterations in oxidative stress parameters. Materials and Methods: Total thiol (T.thl), oxidative stress index (OSI), total oxidant status (TOS) and total antioxidant status (TAS) were examined in addition to the PON1 and ARE enzyme activities in twenty one FLCe-MM and nineteen NFLCe-MM subjects. Routine parameters like lipid panel, serum total protein, albumin, creatinine, blood urea nitrogen (BUN), uric acid and hemoglobin levels were compared with the oxidative stress markers. Results: Serum total protein, BUN, creatinin, and uric acid levels were significantly higher (p = 0.04, p = 0.001, p = 0.001 and p = 0.0022, respectively), while hemoglobin and albumin levels were significantly lower in FLCe-MM patients (p = 0.009 and p = 0.04,respectively). PON1 and ARE activities were significantly lower in patients with FLCe-MM compared to those with NFLCe-MM (p = 0.001 and p = 0.008, respectively). Conclusions: Depending on our results of prognostic markers of MM such as age, hemoglobin, albumin, and creatinine we feel confident to presume FLCe-MM as a subgroup with a worse prognosis. A decrease in PON1 and ARE activities may contribute to the prognosis and may be used as a prognostic tool in MM.


Eren E.,Laboratory of Ataturk Hospital | Ellidag H.Y.,Central Laboratories of Antalya Education and Research Hospital of Ministry of Health | Aydin O.,Laboratories Of Batman Maternity And Childrens Hospital | Ylmaz N.,Central Laboratories of Antalya Education and Research Hospital of Ministry of Health
Journal of Clinical and Diagnostic Research | Year: 2014

Increased oxidative stress, alterations of lipid metabolism and induction of thrombosis have been suggested to be pathogenic links which are present between hyperhomocysteinaemia and atherosclerosis. However, the mechanism by which homocysteine (Hcy) can promote atherogenesis is far from clear and it has been debated. In the presence of cardiovascular risk factors, endothelial dysfunction is the central commodity which converges a plenty of factors, which have been named as atherogenic. Now-a-days, there are only few studies which have presented the correlation between antioxidant enzyme HDL-associated-paraoxonase 1(PON1) and Hcy in atherosclerosis. Both PON 1 and Hcy have been implicated in human diseases which are related to endothelial dysfunction. Although paraoxonases have the ability to hydrolyze a variety of substrates, only one of them, Hcy-thiolactone, is known to occur naturally. It seems very likely that the involvement of Hcy in atherosclerotic disease is mediated through its interactions with PON1. © 2014, Journal of Clinical and Diagnostic Research. All rights reserved.


Aydin O.,Biochemistry Laboratory | Ellidag H.Y.,Central Laboratories of Antalya Education and Research Hospital of Ministry of Health | Kurtulus F.,Neurology Clinic of Antalya Education and Research Hospital of Ministry of Health | Yaman A.,Neurology Clinic of Antalya Education and Research Hospital of Ministry of Health | Yilmaz N.,Central Laboratories of Antalya Education and Research Hospital of Ministry of Health
Biochemia Medica | Year: 2014

Introduction: Oligodendrocytes need iron in processes of energy generation and myelination. However, excessive levels of iron may exert iron induced oxidative stress and thus lead to tissue degeneration. Monitoring oxidative stress will be of paramount importance in follow-up of patients with many diseases including multiple sclerosis (MS). The aim of this study was to measure total anti-oxidative status (TAS), total oxidative status (TOS) and ischemia modified albumin (IMA) in stable relapse remitting MS (RRMS) patients.Materials and methods: Thirty-five RRMS patients (15 males and 20 females; median age 42 (20-55) years) and thirty-five age-sex matched healthy controls (13 males and 22 females; median age 37 (21-60) years) were included in this study. All patients were diagnosed with MS according to the criteria of McDonald.Results: IMA levels were significantly higher in RRMS patients (P < 0.001), while TAS and TOS did not show any significant difference between groups (P = 0.870 and P = 0.460, respectively).Conclusions: Our results suggest IMA as a more efficient serum marker than TAS and TOS in detecting the oxidative stress in MS patients. Serum oxidative stress markers should be included in future study protocols besides clinical and radiological parameters. © 2014 by Croatian Society of Medical Biochemistry and Laboratory Medicine.


Aydin O.,Biochemistry Laboratory | Aydin O.,Antalya Education and Research Hospital | Kurtulus F.,Neurology Clinic of Antalya Education and Research Hospital of Ministry of Health | Ellidag H.Y.,Central Laboratories of Antalya Education and Research Hospital of Ministry of Health | And 2 more authors.
Neurochemical Journal | Year: 2015

As anti-oxidative therapies come into consideration, finding feasible markers of oxidative stress becomes mandatory. We here in this study investigated the levels of major internal antioxidant uric acid (UA), oxidative stress parameters total antioxidant status (TAS), total oxidative status (TOS) in multiple sclerosis (MS) patients compared to controls. Thirty-five stable relapse remitting MS patients (15 males and 20 females; mean age 38 ± 11 years) and thirty-five age-sex matched healthy controls (13 males and 22 females; mean age 38 ± 10 years) were included in this study. All patients were diagnosed with MS according to the criteria of McDonald. Serum UA levels were significantly lower in the MS group (p = 0.03). When MS patients were sub-classified according to gender, female patients showed lower UA levels compared to male patients (p = 0.01). We did not find statistically significant differences in TAS and TOS between patients and controls. Serum UA correlated well with serum TAS as expected. However the correlation was more powerful in MS group (p < 0.0001 in MS group versus p = 0.02 in controls). Interpreting sole measurements of oxidative stress parameters may be deceptive as their values depend on many factors including serum levels of each other. We believe in the superiority of using TAS and TOS as markers of oxidative stress in MS patients as they inform about totals, independent of levels of individual parameters. © 2015, Pleiades Publishing, Ltd.

Discover hidden collaborations