Time filter

Source Type

Kulaksizoglu S.,Central Laboratories of Antalya Education and Research Hospital | Kulaksizoglu B.,Antalya Education and Research Hospital
Neurochemical Journal | Year: 2017

The objective of this study is to investigate the relationship between anthropometric (BMI and waist circumference), metabolic (glucose, insulin, insulin resistance, HbA1c, and lipid profile), psychopathologic (Positive and Negative Syndrome Scale, PANSS) parameters with vitamin D and serum brainderived neurotrophic factor (BDNF) levels in patients with schizophrenia. The study population consisted of 54 healthy control subjects, and 64 volunteer patients, monitored in the psychiatry outpatient clinics of Antalya Education and Research Hospital. Serum glucose, HDL, LDL, triglyceride, total cholesterol levels (spectrophotometric method), HbA1c (HPLC method), insulin, and vitamin 25(OH)D (chemiluminescence method), with HOMA-IR (numerical calculation), and serum BDNF levels (sandwich ELISA, enzymelinked immunosorbent assay) were quantitatively evaluated using respective analytical methods indicated in parentheses. Twenty-seven (42.18%) of 64 schizophrenia patients were diagnosed with MetS. In schizophrenia patients diagnosed with metabolic syndrome (MetS), PANSS-negative and -positive symptom scores were significantly higher, while serum BDNF levels were significantly lower. In patients with schizophrenia, significantly negative correlations were detected between PANSS-negative and -positive symptom scores, and BDNF (p < 0.001 and p < 0.001, respectively), and also between PANSS-negative symptom score and vitamin D (p = 0.022). Lower serum BDNF levels may be related to increases in the possible development of MetS and psychotic symptoms. Decrease in vitamin D levels in schizophrenia patients may be associated with an increase in PANSS-negative symptom scores. In schizophrenia patients with MetS, psychotic symptoms may be more severe. © 2017, Pleiades Publishing, Ltd.


Aykal G.,Central Laboratories of Antalya Education and Research Hospital | Kesapli M.,Antalya Education and Research Hospital | Aydin O.,Batman Maternity and Childrens Hospital | Esen H.,Antalya Education and Research Hospital | And 3 more authors.
Journal of Medical Biochemistry | Year: 2016

Background: After the introduction of modern laboratory instruments and information systems, preanalytic phase is the new field of battle. Errors in preanalytical phase account for approximately half of total errors in clinical laboratory. The objective of this study was to share an experience of an education program that was believed to be successful in decreasing the number of rejected samples received from the Emergency Department (ED). Methods: An education program about laboratory procedures, quality requirements in the laboratory, patient and health-care worker safety was planned by the quality team to be performed on 36 people who were responsible for sample collection in the ED. A questionary which included 11 questions about the preanalytic phase was applied to all the attendees before and after training. The number of rejected samples per million was discovered with right proportion account over the number of accepted and rejected samples to laboratory after and before the training period. Results: Most of the attendees were nurses (n: 22/55%), with over 12 years of experience in general and 2-4 years experience in the ED. Knowledge level of the attendees was calculated before training as 58.9% and after training as 91.8%. While the total rate of sample rejection before training was 2.35% (sigma value 3.37-3.50), the rate after training was 1.56% (sigma value 3.62-3.75). Conclusions: Increasing the knowledge of staff has a direct positive impact on the preanalytic phase. The application of a pre-test was observed to be a feasible tool to shape group specific education programs. © by Güzin Aykal 2016.


Aykal G.,Central Laboratories of Antalya Education and Research Hospital | Guven R.,Antalya Education and Research Hospital | Yein A.,Central Laboratories of Antalya Education and Research Hospital | Ellida H.Y.,Central Laboratories of Antalya Education and Research Hospital | And 2 more authors.
Clinical Laboratory | Year: 2015

Background: The aim of this study is to observe the association between venous thromboembolism (VTE) and oxidative stress and to see if there is a diagnostic value in the oxidative/antioxidative balance parameters like total oxidant status (TOS), total antioxidant status (TAS), paraoxonase-(PONl), and arylesterase (ARE) enzyme activities in this specific disease. Methods: Sixty-nine patients with deep vein thrombosis and/or pulmonary embolism and 40 control subjects were included in the study. Oxidative stress index, total oxidant status, and antioxidant status were examined in addition to the PON1 and ARE enzyme activities in both groups. Results: Serum PON1 and ARE activities were significantly lower in the VTE patients, whereas total oxidant status was higher in patients compared to the controls. Conclusions: This preliminary study showed that oxidative/antioxidative balance shifted towards the oxidative status in venous thromboembolism. ROC analysis results suggested that the parameters used in this study were not good enough to be used in the diagnosis of VTE. © since 2008 All rights reserved. Copyright Clinical Laboratory Publications GmbH


Yilmaz N.,Central laboratories of Antalya Education and Research Hospital | Aydin O.,Central laboratories of Antalya Education and Research Hospital | Yegin A.,Central laboratories of Antalya Education and Research Hospital | Tiltak A.,Neurology clinic of Antalya Education and Research Hospital | Eren E.,Antalya Public Health Center
Clinical Biochemistry | Year: 2011

Objectives: There is strong evidence associating migraine with a variety of comorbid disorders, including cardiovascular disease and stroke, in which oxidative stress seems to be an important underlying mechanism. The aim of the study was to investigate in migraineurs the body oxidant/antioxidant balance and paraoxonase enzyme activities as a measure of HDL functionality. Design and methods: Oxidative stress index, total oxidant status and antioxidant status were examined in addition to the paraoxonase and arylesterase enzyme activities in sixty-two migraineurs and fifty healthy control subjects. Results: Serum arylesterase activities were significantly lower in migraineurs (p = 0.0065), whereas total oxidant status was higher in patients compared to the controls (p = 0.0035). Conclusions: This preliminary study showed that oxidative/antioxidative balance shifted towards the oxidative status in migraine. Moreover, the results also suggested that decreased arylesterase activities might be associated with HDL-related disfunction. © 2011 The Canadian Society of Clinical Chemists.


Ellidag H.Y.,Central Laboratories of Antalya Education and Research Hospital | Eren E.,Antalya Public Health Center | Aydin O.,Central Laboratories of Antalya Education and Research Hospital | Yldrm M.,Oncology Clinic | And 2 more authors.
Medical Principles and Practice | Year: 2013

Objective: To investigate the status of the oxidant/antioxidant balance in patients with multiple myeloma compared to healthy controls. Materials and Methods: This study was conducted on 40 multiple myeloma patients and 40 healthy controls of matched age and sex. Serum total thiol, oxidative stress index (OSI), total oxidant status (TOS), and total antioxidant status (TAS) were measured using colourimetric methods; paraoxonase-1 and arylesterase enzyme activities were also quantified. Results: Serum paraoxonase-1 and arylesterase activities and total thiol levels were significantly lower (p = 0.0001, p = 0.036 and p < 0.0001, respectively), whereas TOS and OSI levels were significantly higher (p < 0.0001 for both parameters) in multiple myeloma patients compared to controls. However, no significant differences in TAS were identified when the two groups were compared. Conclusions: Our findings indicate an impaired oxidative/antioxidative balance in multiple myeloma. We recommend further studies with larger groups to investigate the possible relationship between oxidative stress and the aetiopathogenesis of multiple myeloma. © 2013 S. Karger AG, Basel.


PubMed | Central Laboratories of Antalya Education and Research Hospital
Type: Journal Article | Journal: Medical principles and practice : international journal of the Kuwait University, Health Science Centre | Year: 2013

To investigate the status of the oxidant/antioxidant balance in patients with multiple myeloma compared to healthy controls.This study was conducted on 40 multiple myeloma patients and 40 healthy controls of matched age and sex. Serum total thiol, oxidative stress index (OSI), total oxidant status (TOS), and total antioxidant status (TAS) were measured using colourimetric methods; paraoxonase-1 and arylesterase enzyme activities were also quantified.Serum paraoxonase-1 and arylesterase activities and total thiol levels were significantly lower (p = 0.0001, p = 0.036 and p < 0.0001, respectively), whereas TOS and OSI levels were significantly higher (p < 0.0001 for both parameters) in multiple myeloma patients compared to controls. However, no significant differences in TAS were identified when the two groups were compared.Our findings indicate an impaired oxidative/antioxidative balance in multiple myeloma. We recommend further studies with larger groups to investigate the possible relationship between oxidative stress and the aetiopathogenesis of multiple myeloma.

Loading Central Laboratories of Antalya Education and Research Hospital collaborators
Loading Central Laboratories of Antalya Education and Research Hospital collaborators