ICAR Central Island Agricultural Research Institute

Port Blair, India

ICAR Central Island Agricultural Research Institute

Port Blair, India

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Kaur S.,Indian Agricultural Research Institute | Iquebal M.A.,Indian Agricultural Research Institute | Jaiswal S.,Indian Agricultural Research Institute | Tandon G.,Indian Agricultural Research Institute | And 5 more authors.
Agri Gene | Year: 2016

Even though cultivated rice is highly sensitive to salinity, significant variability exists in the primary and secondary gene-pool of rice with respect to traits of salinity tolerance. Breeding salinity tolerance rice varieties is imperative due to climate change and increasing rice demand for global population. A meta-analysis of plethora of genomic data and published literature available on various genes/factors associated with response to rice salinity and tolerance can be used to enlist selected candidates genes affecting salinity. Such genes can be utilized to identify potential candidate salinity resistance genes from donor rice genotypes and facilitate their transfer to high yielding varieties of rice through marker-assisted breeding. This approach has tremendous advantage over transgenic approach as no bio-safety or regulatory issues are involved in exploiting the variability. Meta-analyses were performed on three datasets viz., rice microarray data of 166 series comprising of 2586 samples, 1228 published research literature in the last one and half decades and RNA-Seq data of 454 and Illumina from Sequence Retrieval Archive (SRA) at NCBI. Among microarray dataset, six salinity related series were finally selected and multi experiment analysis revealed 2289 differentially expressed genes belonging to 44 gene families. Out of these, 13 families viz., AP2-EREBP, AUX/IAA, bZIP, C2H2, bHLH, C3H, HB, HSF, MYB, MYB-related, NAC, Tify and WRKY were selected. Applying various parameters on the published literature data, 13 genes were selected, of which five were common to the different microarray datasets. From RNA-Seq data, total of 751 differentially expressed genes were obtained from 21 gene families, out of which 11 genes were common with those obtained from microarray data and five genes, viz., AP2-EREBP/DREB, MYB, HSF, bZIP and NAC were common to all the three data sets. Based on the results obtained, a total of 31 meta-analyzed genes have been selected and recommended for use in genetic improvement programs aimed at salinity resistance in rice. The meta-analysis of microarray, RNA-Seq and published literature has been successfully used to select 31 best salinity tolerance associated genes which can be exploited by candidate gene approach for targeted introgression through marker assisted breeding. This approach has multi-fold advantages, as it obviates statutory and ecological issues. Such endeavors are more warranted for combating the key abiotic stresses like salinity, whose effects are increasing due to a changing climate. © 2016 Elsevier Inc.


Swarnam T.P.,ICAR Central Island Agricultural Research Institute | Velmurugan A.,ICAR Central Island Agricultural Research Institute | Pandey S.K.,ICAR Central Island Agricultural Research Institute andaman and Nicobar Islands | Dam Roy S.,ICAR Central Island Agricultural Research Institute
Bioresource Technology | Year: 2016

Vermicompost was prepared by five different treatments from relatively resistant coconut husk mixed with either pig slurry or poultry manure. The recovery of vermicompost varied from 35% to 43% and it resulted in significant increase in pH, microbial biomass carbon, macro and micro nutrients concentration. Among the treatments highest relative N (1.6) and K (1.3) recovery were observed for 20% feedstock substitution by pig slurry while poultry manure substitution recorded highest P recovery (2.4). Compost maturity parameters significantly differed and well correlated. The characteristics of different treatments established the maturity indices as C/N 15-20; Cw < 1.8; Cw/Norg < 0.55; Lignin < 10-12; CHA/CFA > 1.5 and HI > 15.0. The manurial value of the coconut husk compost was improved by feedstock substitution with pig slurry (80:20). The results revealed the technical feasibility of converting coconut husk into valuable compost by feedstock substitution with pig slurry. © 2016 Elsevier Ltd.


Someshwar B.,National Research Center for Integrated Pest Management | Israr A.,ICAR Central Institute for Subtropical Horticulture | Kumar T.A.,ICAR Central Island Agricultural Research Institute
Research Journal of Biotechnology | Year: 2016

The biocontrol fungi, Trichoderma are the most important biocontrol agent and have been used extensively worldwide for the management of various plant pathogens. Trichoderma isolates were isolated from different crops rhizosphere of Bay Islands and established antagonistic potential against Sclerotium rolfsii based on phylogenetic analysis of nucleotide sequences. All isolates of Trichoderma spp significantly inhibited the S. rolfsii by dual culture and production of non-volatile inhibitors at 5 and 10% concentrations of culture filtrate. DNA sequences of the isolates included the internal transcribed spacer (ITS) regions of the rDNA cluster (ITS1-ITS2). The 5.8S region was found conserved and much of the sequence variability was due to indels or transition/transversion mutations in the ITS1 and 2 regions. Both the isolates belonged to the T. harzianum clade. These isolates are distinct yet have close genetic similarity with several established strains/isolates of T. harzianum reported worldwide. Correct identification of these bioagents would augment the effective utilization of these fungi of immense agricultural importance in managing the collar rot disease of brinjal. © 2016, Research Journal of BioTechnology. All rights reserved.


PubMed | ICAR Central Island Agricultural Research Institute andaman and Nicobar Islands and ICAR Central Island Agricultural Research Institute
Type: | Journal: Bioresource technology | Year: 2016

Vermicompost was prepared by five different treatments from relatively resistant coconut husk mixed with either pig slurry or poultry manure. The recovery of vermicompost varied from 35% to 43% and it resulted in significant increase in pH, microbial biomass carbon, macro and micro nutrients concentration. Among the treatments highest relative N (1.6) and K (1.3) recovery were observed for 20% feedstock substitution by pig slurry while poultry manure substitution recorded highest P recovery (2.4). Compost maturity parameters significantly differed and well correlated. The characteristics of different treatments established the maturity indices as C/N 15-20; Cw<1.8; Cw/Norg<0.55; Lignin<10-12; CHA/CFA>1.5 and HI>15.0. The manurial value of the coconut husk compost was improved by feedstock substitution with pig slurry (80:20). The results revealed the technical feasibility of converting coconut husk into valuable compost by feedstock substitution with pig slurry.


Singh S.,ICAR Central Island Agricultural Research Institute | Singh S.,Indian Agricultural Research Institute | Waman A.A.,ICAR Central Island Agricultural Research Institute | Bohra P.,ICAR Central Island Agricultural Research Institute | And 2 more authors.
Genetic Resources and Crop Evolution | Year: 2016

Tropical region representing 36 % of the Earth’s surface and 20 % of its land mass is characterized by warm to hot and moist climate with lush green vegetation. Andaman and Nicobar Islands, India harbour over 2650 species of flowering plants, of which 223 species are endemic and 1300 do not occur in the mainland India. Horticultural crops occupy about 70 % of the total cropped area in the islands. Plantation crops (63 %) are dominant component, while vegetables, fruits and tuber crops are known to contribute in livelihood and nutrition of native tribes and settler population. Continuous introductions of non-native crops and domestication of wild plants expended the list of horticultural crops since the Penal Settlement Process in nineteenth century. Presently, about 150 species of vegetables, 120 of fruits, 132 of orchids, 120 of ferns and 300 of medicinal plants have been reported from the islands. By utilizing the diversity in potential species, 18 improved varieties have been developed in various crops apart from identification of 20 breeding lines, registration of seven unique germplasm and conservation of about 187 germplasm at National gene banks. However, overemphasis on commercial exotics, ignorance of native bioresources, introduction of pests, habitat degradation and changing climatic factors could lead to loss of potential horticultural germplasm. Present article highlights efforts made and challenges involved in the conservation and sustainable utilization of horticultural resources for development of the tropical archipelago in the era of changing climate. © 2016 Springer Science+Business Media Dordrecht


Sreesujatha R.M.,Livestock Research and Information Center Sheep | Jeyakumar S.,National Dairy Research Institute | Kundu A.,ICAR Central Island Agricultural Research Institute | Balasundaram C.,Bharathidasan University
Theriogenology | Year: 2016

The present study was undertaken to evaluate the ovarian status of matured breeding female Japanese quail by means of B Mode portable ultrasound scanning. Active ovaries in various stages of hierarchical development and different morphological structure were observed. The large yellow follicles appeared as a cluster of dark circles with various intensity of hyperechoic concentric ring. Sonographically, these follicles were classified into F1, F2, and F3 measuring 16.20 ± 0.06 mm, 12.48 ± 0.09 mm, and 7.95 ± 0.16 mm, respectively. A comparative study on preslaughter and postslaughter ovarian follicular status revealed accuracy of follicles less than 10 mm, 10-15 mm, 15-18 mm, and greater than 18 mm as 26.31%, 78.9%, 93.75%, and 100%, respectively, with an overall accuracy of 71%. This is the first report on the application of transcutaneous ultrasonographic technique to monitor ovarian status on Japanese quail reporting that the ovarian follicular activity and morphological structure can be monitored nonintrusively throughout quail life which can used as model for reproductive and biomedical research. © 2016 Elsevier Inc.


Dhanani T.,ICAR Directorate of Medicinal and Aromatic Plants Research | Singh R.,ICAR Directorate of Medicinal and Aromatic Plants Research | Waman A.,ICAR Central Island Agricultural Research Institute | Patel P.,ICAR Directorate of Medicinal and Aromatic Plants Research | And 2 more authors.
Industrial Crops and Products | Year: 2015

In order to supply uniform, genuine, good quality raw material to the pharmaceutical industries and to reduce the burden on natural stocks, cultivation of medicinal crops is the need of the hour. However, improved varieties with optimum levels of active ingredients have so far not been developed in a number of important medicinal crops. Considering this, an investigation was undertaken to assess the extent of variability amongst the natural populations of Gurmar (. Gymnema sylvestre), an important anti-diabetic plant of the Indian Systems of Medicine. Evaluation of the accessions for morphological parameters revealed highly significant differences for leaf related parameters viz. length, width, fresh as well as dry weight and petiole length, which directly contribute to the biological yield of the plant. Further, a validated high performance liquid chromatography-diode array detection (HPLC-DAD) method was also developed for identification and quantification of bioactive principle i.e., gymnemagenin in a gradient elution mode using solvent mixture composing of acetonitrile (solvent A), potassium dihydrogen orthophosphate (10. mM, solvent B) both solvent A and B containing orthophosphoric acid (0.05%, v/v). Results revealed significant differences for gymnemagenin content amongst the accessions evaluated. These accessions could be used in breeding programs for development of cultivars with optimum levels of gymnemagenin, which in turn may promote the cultivation of this high value medicinal crop. © 2015 Elsevier B.V..


Waman A.A.,University of Agricultural Sciences, Bangalore | Waman A.A.,ICAR Central Island Agricultural Research Institute | Bohra P.,University of Agricultural Sciences, Bangalore | Bohra P.,ICAR Central Island Agricultural Research Institute | Sathyanarayana B.N.,University of Agricultural Sciences, Bangalore
Indian Journal of Plant Physiology | Year: 2016

Separated bud clumps of Silk Banana containing single and double buds were transferred on media containing six different cytokinins at three different levels and the proliferation capacity was determined. Proliferation, though occurred in single bud explants, the rate of multiplication was lower when compared with double bud explants, irrespective of the type of cytokinin used. Maximum shoot proliferation was observed in explants cultured on media supplemented with thidiazuron and 2-isopentenyl adenine (2-iP), and was significantly superior when combined with kinetin and BAP. Meta-topolin though reported to be highly efficient in improving multiplication in other banana varieties, no appreciable response was observed during present investigation. Use of NAA (2 mg l−1) was found to promote rooting process with superior root induction parameters and survival. © 2016, Indian Society for Plant Physiology.


PubMed | ICAR Central Island Agricultural Research Institute
Type: Journal Article | Journal: Theriogenology | Year: 2016

The present study was undertaken to evaluate the ovarian status of matured breeding female Japanese quail by means of B Mode portable ultrasound scanning. Active ovaries in various stages of hierarchical development and different morphological structure were observed. The large yellow follicles appeared as a cluster of dark circles with various intensity of hyperechoic concentric ring. Sonographically, these follicles were classified into F1, F2, and F3 measuring 16.200.06mm, 12.480.09mm, and 7.950.16mm, respectively. A comparative study on preslaughter and postslaughter ovarian follicular status revealed accuracy of follicles less than 10mm, 10-15mm, 15-18mm, and greater than 18mm as 26.31%, 78.9%, 93.75%, and 100%, respectively, with an overall accuracy of 71%. This is the first report on the application of transcutaneous ultrasonographic technique to monitor ovarian status on Japanese quail reporting that the ovarian follicular activity and morphological structure can be monitored nonintrusively throughout quail life which can used as model for reproductive and biomedical research.


Singh S.,ICAR Central Island Agricultural Research Institute | Singh P.K.,ICAR Central Island Agricultural Research Institute | Singh D.R.,ICAR Central Island Agricultural Research Institute | Pandey V.B.,ICAR Central Island Agricultural Research Institute | Srivastava R.C.,ICAR Central Island Agricultural Research Institute
Indian Journal of Horticulture | Year: 2015

Twenty four dolichos bean genotypes from different parts of India were evaluated for ten quantitative traits in Andaman Islands. Moderate to low genotypic and phenotypic coefficients of variation were recorded for all the traits. High heritability (70.97%) and high genetic advance (74.96%) were observed for plant height, while high heritability (64.24%) but low genetic advance (2.58%) were recorded for pod length. Pod length (r = 0.611), number of seeds per pod (r = 0.764), number of pods per plant (r = 1.00) and pod yield per plant (r = 1.00) had positive and significant correlation with pod yield. Path coefficient analysis revealed maximum direct effect of days to 50% flowering, pod width, number of pods per plant and pod yield per plant on pod yield indicating traits of importance for yield improvement. © 2015, Horticulture Society of India. All rights reserved.

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