Kaur P.,Guru Angad Dev Veterinary and Animal Sciences University |
Filia G.,Guru Angad Dev Veterinary and Animal Sciences University |
Singh S.V.,Central institute of research on goats |
Patil P.K.,Guru Angad Dev Veterinary and Animal Sciences University |
Sandhu K.S.,Guru Angad Dev Veterinary and Animal Sciences University
Tropical Animal Health and Production | Year: 2010
Genotyping of Mycobacterium avium subspecies paratuberculosis (MAP) is important for precise classification of bacterium and for understanding the molecular epidemiology. The present study reports detection and typing of the MAP from milk. On the basis of clinical signs of diarrhea and/or weakness, the dairy animals suspected for Johne's disease were screened by Ziehl-Neelsen staining of fecal samples. The milk samples from 13 selected animals were processed for DNA extraction and direct IS900 polymerase chain reaction (PCR). MAP identified by IS900 PCR was genotyped using IS1311 PCR-restriction endonuclease analysis (REA). IS900 milk PCR revealed 30. 8% animals positive for MAP, including 40% of the moderate and 50% of the heavy fecal shedders. All infected animals showed Bison type MAP in IS1311 PCR-REA. IS900 PCR can be used for screening of milk for MAP; however, the method needs to be evaluated for subclinical cases. IS1311 PCR-REA results indicated the predominance of Bison type MAP in the dairy animals of this region. © 2010 Springer Science+Business Media B.V.
Bharti V.K.,Central Institute of Research on Goats |
Singh S.P.,Central Institute of Research on Goats |
Kumar P.,Central Institute of Research on Goats |
Kumar P.,Indian Veterinary Research Institute |
And 2 more authors.
Indian Journal of Animal Sciences | Year: 2012
During solar eclipse, unusual disturbances in the circadian rhythm affects the metabolism of animals through change in their biological clock. Therefore, the present study was designed to know the effects of solar eclipse on certain serum biochemicals of goats under intensive and extensive housing systems. Experiment was started from 19 July 2009 (control) to study the effects of solar eclipse (22July 2009). Adult goats (24) of both sexes were divided into 4 groups of male and female, viz. group1 (male extensive), group 2 (female extensive), group 3 (male intensive), and group 4 (female intensive). They were provided ration and water ad lib. during entire period of investigation. All the experimental goats were housed in extensive (groups 1 and 2) and intensive (groups 3 and 4) housing system to know the protective effect of different housing during solar eclipse. Daily (19-23 July 2009), we collected blood samples for analysis of serum T3, T4, thyroid stimulating hormone (TSH), alkaline phosphatase (ALP), alanine aminotransferase (ALT), Ca, and P. After solar eclipse (23 July 2009), TSH level was significantly increased in group 3. Serum ALT activity was significantly low in group1 on day of solar eclipse and end of experiment, whereas low activity of ALT was recorded in group 3 and4 at the end of experiment. Interestingly, high level of Ca in group 3 as well as ALP in group1 and 3 was recorded on solar eclipse day and was persistent till the end of experiment. However, serum T3 was significantly decreased in group 3 on solar eclipse day at the end of experiment. These findings are interesting and noticable in contest of observation on certain serum biochemical profile of goats during solar eclipse under different housing systems, which require further exploration.
Mukherjee R.,Indian Veterinary Research Institute |
Jadhav R.K.,Indian Veterinary Research Institute |
Sharma N.,Central Institute of Research on Goats |
Sahu B.D.,Indian Veterinary Research Institute |
De U.K.,Indian Veterinary Research Institute
Buffalo Bulletin | Year: 2014
Somatic cell count (SCC), total bacterial count (TBC), L selectin and CD 18 on milk leukocytes was studied in buffaloes inflicted with intramammary infection in response to Curcuma longa (C. longa, CL) and vitamin E plus selenium (group II), Enrofloxacin (group III) and sterile PBS (group IV). Significant reduction (P<0.05) in SCC, TBC was observed in post-treated buffalo cows. The mean fluorescent intensity (MFI) of L selectin increased significantly (P<0.05) in Group 2 post- treated cows; however, there was no reduction in CD 18 counts in this group. The results suggest that Curcuma longa (CL) possesses antibacterial, anti- inflammatory and immunomodulatory properties. In the present study the biological activity of the CL and vitamin E plus selenium at standardized dose against mastitis in buffalo cows is reported for the first time. Development of alternative therapy is an option for livestock farmers who are not allowed to use allopathic drugs under certain farming system. © 2014, Kasetsart University. All rights reserved.