Central Institute of Fresh Water Aquaculture

Bhubaneshwar, India

Central Institute of Fresh Water Aquaculture

Bhubaneshwar, India
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Kathirvel K.,Central Institute of Fresh Water Aquaculture | Behera P.C.,Central Institute of Fresh Water Aquaculture | Pallai S.,Central Institute of Fresh Water Aquaculture | Mohanty J.,Central Institute of Fresh Water Aquaculture | Parija S.C.,Orissa University of Agriculture and Technology
International Journal of Drug Development and Research | Year: 2010

Pharmacological and Reverse Transcription-Polymerase Chain Reaction (RT-PCR) experiments were performed in goat ruminal artery to determine the pharmacological properties of α1-adrenoceptor present in goat ruminal artery. Ruminal artery was collected in chilled aerated modified's Kreb's Henseleit Solution (KHS) from local slaughter house and transported in ice to laboratory for further processing. The arterial ring was mounted in thermostatically controlled (37 ± 0.5 °C) automatic organ bath containing 20 ml of Modified KHS, bubbled with air (pH, 7.4) under 2g tension. The equilibration of 90 min was allowed after that drug was added. Nor-adrenaline (10 nM - 10 μM) induced a concentration related contractile response with mean EC50 value of 0.73 μM and pD2 value of 5.86 ± 0.05. Prazosin (0.01/0.1/ 1 nM), a nonselective α1-adrenoceptor antagonist caused a significant rightward shift of the NA-induced concentration-related response curve. The estimated pA 2 for prazosin in this tissue was 10.9± 0.03. The RT-PCR analysis of mRNA of goat ruminal artery showed transcript for α1a/d-adrenoceptor. The present study proposed that the vasotonicity mediated by NA in goat ruminal artery is due to presence of α1a/d-adrenoceptor subtypes. © 2010 IJDDR.


Bej D.,Central Institute of Fresh Water Aquaculture | Sahoo L.,Central Institute of Fresh Water Aquaculture | Das S.P.,Central Institute of Fresh Water Aquaculture | Swain S.,Central Institute of Fresh Water Aquaculture | And 6 more authors.
Mitochondrial DNA | Year: 2012

The complete mitochondrial genome of Labeo rohita, an important cultivable fish, was determined for the first time. The genome is 16,611 bp in length and consists of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and one control region. The gene organisation and its order were similar to other vertebrates. The overall base composition on heavy strand was as follows A: 32.5%, G: 15.2%, C: 27.7%, T: 24.47%, and the AT content 56.9%. The control region contains a microsatellite, (TA)12, a putative termination-associated sequence and three conserved sequence blocks. This mitogenome sequence data would play an important role in population genetics and phylogenetics of Indian major carps. © 2012 Informa UK, Ltd.


PubMed | Central Institute of Fresh Water Aquaculture
Type: Journal Article | Journal: Mitochondrial DNA | Year: 2012

The complete mitochondrial genome of Labeo rohita, an important cultivable fish, was determined for the first time. The genome is 16,611bp in length and consists of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and one control region. The gene organisation and its order were similar to other vertebrates. The overall base composition on heavy strand was as follows A: 32.5%, G: 15.2%, C: 27.7%, T: 24.47%, and the A+T content 56.9%. The control region contains a microsatellite, (TA)(12), a putative termination-associated sequence and three conserved sequence blocks. This mitogenome sequence data would play an important role in population genetics and phylogenetics of Indian major carps.

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