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The Central Institute of Fisheries Education is the only Deemed University for fisheries science in India. It is an institution of higher learning for fisheries science in India. CIFE has over four decades of leadership in HRD with its alumni aiding the development of fisheries and aquaculture world wide, and has notable research and technological advancements to its credit.The institute is one of the four main institutes under the Indian Council for Agricultural Research ; the other three being the Indian Veterinary Research Institute , The National Dairy Research Institute and the Indian Agriculture Research Institute A total of four subcentres operate under CIFE, including ones at Kolkata , Rohtak , Kakinada and Powerkheda . Wikipedia.

Makesh M.,University of Idaho | Makesh M.,Central Institute of Fisheries Education | Sudheesh P.S.,University of Idaho | Cain K.D.,University of Idaho
Fish and Shellfish Immunology | Year: 2015

Teleosts possess three immunoglobulin (Ig) heavy chain isotypes viz., IgM, IgT and IgD and all three isotypes are reported in rainbow trout. The expression of these Ig isotypes in response to different immunization routes was investigated and results provide a better understanding of the role these Igs in different tissues. Rainbow trout (Oncorhynchus mykiss) were immunized with an attenuated Flavobacterium psychrophilum strain, 259-93-B.17 grown under iron limiting conditions, by intraperitoneal, anal intubation and immersion routes. Serum, gill mucus, skin mucus and intestinal mucus samples were collected at 0, 3, 7, 14, 28, 42 and 56 days post immunization by sacrificing four fish from each treatment group and the unimmunized control group, and the IgM levels were estimated by an enzyme linked immunosorbent assay (ELISA). In addition, blood, gill, skin and intestinal tissue samples were collected for Ig gene expression studies. The secretory IgM, IgD and IgT gene expression levels in these tissues were estimated by reverse transcription quantitative real time PCR (RT-qPCR). Levels of IgM in serum, gill and skin mucus increased significantly by 28 days after immunization in the intraperitoneally immunized group, while no significant increase in IgM level was observed in fish groups immunized by other routes. Secretory IgD and IgT expression levels were significantly upregulated in gills of fish immunized by the immersion route. Similarly, secretory IgT and IgD were upregulated in intestines of fish immunized by anal intubation route. The results confirm mucosal association of IgT and suggest that IgD may also be specialized in mucosal immunity and contribute to immediate protection to the fish at mucosal surfaces. © 2015 Elsevier Ltd.

Kumar S.,Central Institute of Fisheries Education | Varela M.F.,Eastern New Mexico University
International Journal of Molecular Sciences | Year: 2012

Bacterial pathogens that are multi-drug resistant compromise the effectiveness of treatment when they are the causative agents of infectious disease. These multi-drug resistance mechanisms allow bacteria to survive in the presence of clinically useful antimicrobial agents, thus reducing the efficacy of chemotherapy towards infectious disease. Importantly, active multi-drug efflux is a major mechanism for bacterial pathogen drug resistance. Therefore, because of their overwhelming presence in bacterial pathogens, these active multi-drug efflux mechanisms remain a major area of intense study, so that ultimately measures may be discovered to inhibit these active multi-drug efflux pumps. © 2012 by the authors; licensee MDPI, Basel, Switzerland.

Panda S.,Central Institute of Fisheries Education
Journal of Food Processing and Preservation | Year: 2016

The effect of seaweed incorporation on the quality of fish jerky was studied. Sargassum wightii was incorporated in proportions of 0, 3, and 5% in Tuna jerky batter formulation. Physicochemical, microbiological, antioxidant, and sensory qualities were assessed. Total fiber content increases from 0.91 to 2.49% in seaweed incorporated sample. The additional seaweed produced samples with less tensile strength. The results showed that addition of seaweed increase the amounts of the macro minerals and trace elements in the jerky. Addition of seaweed improves antioxidant quality by decreasing IC50-DPPH of the product from 0.98 to 0.65mg/ml. Addition of seaweed up to 3% did not affect the organoleptic quality. Sargassum wightii can be a potent functional ingredient in convenient food like jerky. Practical Applications: Jerky is a convenient ready-to-eat dried product, shelf-stable at room temperature. Jerky is generally made by mixing comminuted meat with appropriate seasonings. Dried seaweed powder can be an ingredient in such products as seaweeds are used extensively as food in South East Asian countries. Seaweeds included as components of fish products would improve nutritional quality and also induce positive effects on health. This study was taken up to combine the positive effect of seaweed to dry-fish product like jerky. © 2016 Wiley Periodicals, Inc.

Kumar R.,Central Institute of Fisheries Education
Journal of AOAC International | Year: 2010

Seafood, including fish, shrimp, clam, crab, mussel, oyster, lobster, squid, octopus, and cuttlefish samples, was used to compare the recovery of Salmonella serovars by different selective enrichment and isolation media. Thesamples were selectively enriched in Rappaport-Vassiliadis (RV) broth and tetrathionate broth (TT), followed by selective isolation on Hektoen enteric (HE) agar, xylose lysine desoxycholate (XLD) agar, bismuth sulfite (BS) agar, and Brilliant Green (BG) agar media. Of 443 seafood samples analyzed, 108 were found to be contaminated with Salmonella. The role of selective enrichment in Salmonella spp. recovery with RV medium was distinctly high (70%) compared to TT broth (30%). The selective enrichment in RV broth followed by selective isolation on XLD, HE, BS, and BG agar recovered Salmonella at levels of 56, 41, 28, and 16%, respectively. Similarly, after enrichment in TT broth, XLD and HE agars recovered 27 and 23% respectively. The recovery of Salmonella with enrichment in TT followed by isolation on BS and BG was abysmally low at 4.6 and 5%, respectively.There was no significant difference (P > 0.05) in the recovery of Salmonella using the combinations of XLD and HE media with selective enrichment in RV broth. However, performance difference (P <0.05) was observed in the recovery when BS and BG with RV, and XLD, HE, BS, and BG agars with TT broth were used. The present study showed that the combination of RV with XLD was the most efficient media for isolation of Salmonella from seafood when compared to other isolation media combinations.

Gireesh-Babu P.,Central Institute of Fisheries Education
Molecular biology reports | Year: 2012

Bacterial biosensors can measure pollution in terms of their actual toxicity to living organisms. A recombinant bacterial biosensor has been constructed that is known to respond to toxic levels of Zn(2+), Cd(2+) and Hg(2+). The zinc regulatory gene zntR and zntA promoter from znt operon of E. coli have been used to trigger the expression of GFP reporter protein at toxic levels of these ions. The sensor was induced with 3-800 ppm of Zn(2+), 0.005-4 ppm of Cd(2+) and 0.001-0.12 ppm of Hg(2+) ions. Induction studies were also performed in liquid media to quantify GFP fluorescence using fluorimeter. To determine the optimum culture conditions three different incubation periods (16, 20 and 24 h) were followed. Results showed an increased and consistent fluorescence in cells incubated for 16 h. Maximum induction for Zn(2+), Cd(2+) and Hg(2+) was observed at 20, 0.005 and 0.002 ppm, respectively. The pPROBE-zntR-zntA biosensor reported here can be employed as a primary screening technique for aquatic heavy metal pollution.

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