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Sophia S.,Health Science University | Ramesha M.M.,Central Institute of Brackishwater Aquaculture ICAR
Asian Journal of Microbiology, Biotechnology and Environmental Sciences | Year: 2015

Present study was carried out to find the correlation between antioxidant enzyme status and antibody response to tumor specific antigen in serum of the skin carcinoma mice. Skin carcinoma was induced using 7,12-Dimethylbenz [a] anthracene; the mice at different stages of tumor development were diagnosed using histopathological analysis and selected for the study. Reduced glutathione and glutathione-S-transferase enzymes were measured from the crude enzyme fraction from freshly dissected liver sample. Tumor specific antigen was isolated using SDS-PAGE and autoantibody in the serum was meseared using ELISA. There was significant increase in the enzyme activity during the early stage of tumor progress and the level of auto antibodies to the tumor specific antigen was significantly high during the early progressive stages when compared with later stages. The increased activities of antioxidant enzymes may be compensatory regulation in response to increased oxidative stress during the early and progressive stages. Hence treatment with antioxidants in the initial stages of the disease may be useful as secondary therapy to prevent the oxidative damage and to improve the immune function. The results also insist the necessity to find out the antioxidant status of each patient for therapeutic co-administration of antioxidants along with chemotherapy. © Global Science Publications. Source


Mahalakshmi P.,Vellore Institute of Technology | Mahalakshmi P.,Central Institute of Brackishwater Aquaculture ICAR | Ganesan K.,Vellore Institute of Technology | Venkatasubramanian V.,ICAR
Aquaculture International | Year: 2012

Decision making tool called Decision Making Tool for Identification of Optimal Location in Aqua farming development was developed using Visual Basic programming language for identification of optimal location for aquaculture farming development. Twenty-four input criteria to the tool were categorized into six broad heads of main-criteria namely: water (10 sub-criteria), soil (5 sub-criteria), support (2 sub-criteria), infrastructure (3 sub-criteria), input (1 sub-criterion), and risk factor (3 sub-criteria). In this tool, the optimal location was identified based on the objective function, which was derived from the combination of rank sum, pair-wise comparison, and Technique for Order Preference by Similarity to the Ideal Solution methods. Based on the objective function values, alternatives (or aqua farms) were ranked in descending order in a table. In order to validate the developed tool, the same alternatives were ranked in descending order according to the observed average yield value per hectare (ha) for the last three crops. Spearman's rank correlation was used to assess the correlation between ranks obtained by the tool and ranks obtained based on average yield. It showed with 99% confidence that a significant correlation exists between ranks obtained by the tool and ranks obtained based on average yield. Developed tool appeared to be confident and robust in proof-of-concept application for aquaculture farming development in Kalla mandal with reference to shrimp farms, West Godavari district, Andhra Pradesh, India. © 2012 Springer Science+Business Media B.V. Source


Sawant P.B.,Central Institute of Fisheries Education | Bera A.,Central Institute of Fisheries Education | Bera A.,Central Institute of Brackishwater Aquaculture ICAR | Dasgupta S.,Central Institute of Fisheries Education | And 3 more authors.
PLoS ONE | Year: 2014

Hypoxia is a global phenomenon affecting recruitment as well as the embryonic development of aquatic fauna. The present study depicts hypoxia induced disruption of the intrinsic pathway of programmed cell death (PCD), leading to embryonic malformation in the goldfish, Carrasius auratus. Constant hypoxia induced the early expression of pro-apoptotic/tumor suppressor p53 and concomitant expression of the cell death molecule, caspase-3, leading to high level of DNA damage and cell death in hypoxic embryos, as compared to normoxic ones. As a result, the former showed delayed 4 and 64 celled stages and a delay in appearance of epiboly stage. Expression of p53 efficiently switched off expression of the antiapoptotic Bcl-2 during the initial 12 hours post fertilization (hpf) and caused embryonic cell death. However, after 12 hours, simultaneous downregulation of p53 and Caspase-3 and exponential increase of Bcl-2, caused uncontrolled cell proliferation and prevented essential programmed cell death (PCD), ultimately resulting in significant (p<0.05) embryonic malformation up to 144 hpf. Evidences suggest that uncontrolled cell proliferation after 12 hpf may have been due to downregulation of p53 abundance, which in turn has an influence on upregulation of anti-apoptotic Bcl-2. Therefore, we have been able to show for the first time and propose that hypoxia induced downregulation of p53 beyond 12 hpf, disrupts PCD and leads to failure in normal differentiation, causing malformation in gold fish embryos. © 2014 Sawant et al. Source


Anand P.S.S.,Central Institute of Brackishwater Aquaculture ICAR | Kohli M.P.S.,Central Institute of Fisheries Education | Kumar S.,Central Institute of Brackishwater Aquaculture ICAR | Sundaray J.K.,Indian Central Institute of Freshwater Aquaculture | And 4 more authors.
Aquaculture | Year: 2014

A 60-day indoor growth trial was conducted to study the effect of dietary supplementation of biofloc on growth performance and digestive enzyme activities in Penaeus monodon juveniles. Biofloc developed in indoor fiberglass reinforced plastic (FRP) tanks (1000. L) was used as dietary supplement for P. monodon (2.90 ± 0.10 g) reared in 1000. L FRP tanks. Graded level of dried biofloc was included in shrimp basal diets; 0 (B0, control), 4 (B4), 8 (B8) and 12% (B12). The dried biofloc contained 24.30 ± 0.28% protein. Fatty acid profile of biofloc revealed palmitic acid (46.54%), cis-Vaccenic acid (15.37%), linoleic acid (10.67%) and oleic acid (9.19%) as major fatty acids. There were 16.9 and 13.9% significantly higher (p < 0.01) final body weights in B8 and B4 respectively compared with control, B0. Similarly, significantly better (p < 0.05) feed conversion ratio (FCR), 1.84 ± 0.09 and protein efficiency ratio (PER), 3.48 ± 0.17 was noticed in B4 compared to control (FCR 2.29 ± 0.11 and PER 2.80 ± 0.13). At the end of the feeding trial, B4 recorded 57.6, 45.5, 61 and 78.6% significant increases (p < 0.01) in hepatopancreas digestive enzyme activities for amylase, cellulase, lipase and protease respectively compared with control. However, treatment with 12% level of biofloc inclusion (B12) did not differ significantly (p > 0.05) for most of the growth performance parameters and digestive enzyme activities compared with control. The present study elucidates the suitability of biofloc as a dietary supplement at 4% level in shrimp feed for enhancing growth and digestive enzyme activities in tiger shrimp juvenile. © 2013 Elsevier B.V. Source


Anand P.S.S.,Central Institute of Brackishwater Aquaculture ICAR | Kohli M.P.S.,Central Institute of Fisheries Education | Roy S.D.,Central Agricultural Research Institute | Sundaray J.K.,Central Institute of Brackishwater Aquaculture ICAR | And 4 more authors.
Aquaculture | Year: 2013

A 60-day indoor growth trial was conducted to study the effect of dietary supplementation of periphyton on growth performance and digestive enzyme activities in Penaeus monodon juvenile. Periphyton developed over bamboo substrate in outdoor cistern tanks (15m2) was used as a dietary supplement in P. monodon (2.02±0.04g) reared in 1000l fiberglass reinforced plastic tank. Graded level of dried periphyton was included in shrimp basal diets; 0% (control), 3% (T1), 6% (T2), 9% (T3) and a control diet with natural bamboo substrate for periphyton growth (T4) served as a positive control. Significantly lower (p<0.01) level of NO3-N, and PO4-P were noticed in T4 compared to other experimental groups, while no significant difference in other water quality parameters were noticed among the treatments. At the end of the feeding trial, significantly higher (p<0.01) body weight was noticed in T2 (5.50±0.21g) and T4 (5.33±0.13g) compared with control (4.44±0.17g). Similarly, significantly better (p<0.01) feed conversion ratio (FCR), 2.01±0.08 and protein efficiency ratio (PER), 2.70±0.12 was noticed in T2 compared to control (FCR 2.81±0.19 and PER 1.94±0.14) and T3 (FCR 3.58±0.14 and PER 1.51±0.06), while no significant difference was noticed between T4 and T1. At the end of the feeding trial, there was 75.45, 128.3, 178.5 and 76.6% significant increase (p<0.05) in gut digestive enzymes amylase, cellulase, protease and trypsin respectively in T2 compared with control. Similarly, most of the digestive enzyme activities in T1 and T4 were superior compared to control and T3. However, treatment with 9% level of periphyton inclusion (T3) did not differ significantly (p>0.05) for most of the digestive enzyme activities, growth, FCR and PER compared with control indicating inherent upper limit of periphyton inclusion. The present study elucidates the suitability of periphyton as a novel dietary supplement and its supplementation up to 6% in shrimp for enhancing growth and digestive enzyme activities in tiger shrimp juvenile. © 2013 Elsevier B.V. Source

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