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Karthik D.,University | Vijayakumar R.,Central Institute of Brackishwater Aquaculture CIBA ICAR | Pazhanichamy K.,Periyar Maniammai University | Ravikumar S.,University
Acta Biochimica Polonica | Year: 2014

Background: Cardiovascular proteomics investigation reveals the characterization and elucidation of the novel therapeutic targets and strategies to prevent the development of heart failure associated diabetic complication by using 2DE and MS. Methods: The experimental animals were made diabetic with a single intraperitoneal injection of alloxan (150 mg/kg of bw). Albino rats were randomly divided into four individual groups: Group-I control (n=6), group-II alloxan-induced diabetic rats, untreated (n=6), group-III (n=6) and group-IV (n=6) alloxaninduced diabetic rats were treated with aqueous and ethanolic extracts of Cynodon dactylon for 15 days, respectively. Animals were euthanized to collect the heart tissues and blood samples. 2DE sample preparation, gel running and staining (n=6: each groups) were performed at the same time to avoid variation. The result of six gel images from each group were analyzed and evaluated as one match set with 2D software (P<0.05). Results: The above experiment revealed two up-regulated proteins in group-II i.e. NTF4 and ETFB. Conclusions: NTF4 is a neuro-protective agent for neuro-degenerative diseases. It will prevent diabetic secondary complications, such as diabetic polyneuropathy and cardiomyopathy. ETFB is active in the mitochondria, the energy-producing centres in cells. It is clear from the experiment that because of up-regulation of ETFB more energy is availabile and the electron transfer for heart during diabetes is possible, what leads to reduce the oxidative stress and free-radical formation. The up-regulated proteins reduced CVD that occurred just before overt hyperglycaemia due to administration of C. dactylon. This approach established the preliminary reference map for decoding cellular mechanisms linked between pathogenesis CVD and diabetes.

Sudalayandi K.,Ohio State University | Sudalayandi K.,Central Institute of Brackishwater Aquaculture CIBA ICAR | Kumar A.,Central Institute of Brackishwater Aquaculture CIBA ICAR | Sessler R.,Central Institute of Brackishwater Aquaculture CIBA ICAR | And 5 more authors.
Pakistan Journal of Botany | Year: 2012

Chlamydomonas reinhardtii, a wild-type algal strain (CC 2137) was grown on tris acetate phosphate agar (TAP) medium. The growth curve values showed 2.0 logs till 6 days and declined thereafter. Later, the cells were harvested and extracted for fat using chloroform and methanol (2:1). The extract was hydrolyzed, lyzed and found to have unsaturated fatty acids like linolenic, linoleic, oleic acid, palmitoleic acid and saturated fatty acids viz. myristic, palmitic and stearic acid,using gas chromatography as well as mass spectrometry (GC-MS). Cell wall bound iron containing fatty acid elongation (FAE1) proteins of CC2137 were extracted and their size (ranging from 25-250 KD) was detected by SDS PAGE. The FAE1 protein was inhibitory to Bacillus cereus as evidenced by "Agar Spot Assay". Organic extracts of CC2137 cells with hot and cold extraction were tested against marine bacteria. In cold extraction, methanolic extracts showed highest inhibition (11.0 mm) to Vibrio harveyi (MM30) and subsequently acetone and ethyl acetate extracts showed inhibition to V. harveyi (MM32). In hot extraction, highest inhibitions were observed by ethanol and ethyl acetate (13.0 mm) to MM30 followed by methanol, ethanol and ethyl acetate extracts (12.0 mm) to MM32. Extracts of ethyl acetate and acetic acid showed maximum and identical inhibitions (8.0 mm) against Pseudomonas putida (PP) and lowest by methanoles and chloroform (6.0 mm) in cold extraction. Under hot extraction, the highest inhibition was shown by ethanol extract to PP followed by methanol and acetone (11.0 mm) and lowest by chloroform and acetic acid extracts (6.0 mm).

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