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Otta S.K.,Central Institute of Brackishwater Aquaculture and 75 Santhome High Road
Indian Journal of Virology | Year: 2012

Protein-protein interaction studies have been widely used in several fields to characterize an unknown protein. This in turn helps to find out several pathways to understand a complex mechanism or discover a drug for treatment. Among the methods, yeast two-hybrid has widely been used in human, animal and plant research studies. This aspect of research has also been found useful in understanding the shrimp virus gene function. With respect to White spot syndrome virus, interaction studies have been applied to elucidate virus structure, understand the mode of entry of the virus, mechanism of virus replication and also to discover some of the host anti-viral proteins. Interaction studies on other shrimp viruses are scanty and only few reports available on Yellow head virus and Taura syndrome virus. All these findings are still in preliminary stage and lot more studies are necessary to have the clear picture. Protein interaction research on other shrimp viruses are still lacking. Considering all these, it appears that this field of research has a wide scope to understand the virulence mechanism of shrimp viruses where very little information is available till date. © 2012 Indian Virological Society.


Dineshkumar N.,Central Institute of Brackishwater Aquaculture and 75 Santhome High Road | Saravanakumar C.,Central Institute of Brackishwater Aquaculture and 75 Santhome High Road | Vasanth M.,Central Institute of Brackishwater Aquaculture and 75 Santhome High Road | Muralidhar M.,Central Institute of Brackishwater Aquaculture and 75 Santhome High Road | Alavandi S.V.,Central Institute of Brackishwater Aquaculture and 75 Santhome High Road
International Biodeterioration and Biodegradation | Year: 2014

Denitrifying bacteria in brackishwater shrimp culture pond sediments were isolated and characterized from a total of eight samples. Out of the 264 isolates obtained, only 108 (40.96%) were positive for nitrate reduction and only 14 (12.96%) were positive for both nitrate and nitrite reduction. These fourteen isolates have been characterized based on complete reduction of nitrate to gas, presence of denitrification genes (nirS, nirK and nosZ), reverse transcriptase PCR for nirS gene and quantification of nitrous oxide after blocking the nitrous oxide reductase with acetylene. Out of the 14 isolates, nine were identified as Marinobacter spp., and the others belonged to Shewanella sp., Aquamicrobium sp., Marinimicrobium sp., Microbulbifer sp. and Janibacter sp. None of the denitrification genes could be detected in two of the isolates (CDN1 and 12) that reduced both nitrate and nitrite. RT-PCR analysis of all the Marinobacter isolates showed that nirS gene expression is better under anoxic than under oxic conditions indicating their ability to adapt to varying dissolved oxygen concentration. Results revealed that all the Marinobacter isolates are capable of denitrification under oxic, oxygen tolerant and anoxic conditions and indicated that nirS gene containing bacteria of the order Alteromonadales are one of the predominant denitrifying bacteria in brackishwater shrimp culture ponds of India. The study highlights the significance of such brackishwater ecosystems as study sites for future investigations on distribution and diversity of denitrifying bacteria and their role in the nitrogen cycle. © 2014 Elsevier Ltd.

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