Srivastava N.,Central Institute for Subtropical Horticulture CISH |
Gupta V.K.,NUI |
Gupta V.K.,University of Rajasthan |
Pati R.,Central Institute for Subtropical Horticulture CISH |
Gaur R.K.,University of Rajasthan
Biotechnology | Year: 2011
Genetically modified transgenic crops also referred to as a Genetically Modified (GM) crops are produced by when the genetic material of an organism (either DNA or RNA) is altered by use of recombinant DNA technology and the modification can be replicated and/or transferred to other cells or organisms. The recently understood recombinant DNA technology has potential, via genetic engineering, to incorporate a specific gene which controls a particular trait, without co-transfer of undesirable genes from donor species as occurs in conventional breeding. In recent year, the globally cultivated area of transgenics crops has increased more than 81 million hectares. Most GM crops grown today have been developed to resist certain insect pests. There are GM plants being developed today to produce specific vitamins, resist plant viruses and even produce products for medicinal uses. © 2011 Asian Network for Scientific Information.
Kumar S.,Central Institute for Subtropical Horticulture CISH |
Muthukumar M.,Central Institute for Subtropical Horticulture CISH |
Kumar R.,Central Institute for Subtropical Horticulture CISH |
Bajpai A.,Central Institute for Subtropical Horticulture CISH
Plant Archives | Year: 2012
High quality genomic DNA is the basic requirement for genome analysis in plant and animal tissues. DNA extraction in recalcitrant litchi is difficult because of the high polyphenols, tannins and polysaccharides present in its leaf tissues. Therefore, a modified protocol is reported here, which is an improvement over the existing CTAB method. It involves double extraction using 3.5% CTAB in the initial cell lysis, followed by RNase treatment and an additional silica column purification as final step. This protocol not only resulted in higher yield (Mean DNA concentration=199.68 ng/μl) but also superior quality DNA from 10 litchi cultivars evident from the purity indices, A260/A280 ranging between 1.72±0.14 to 2.00±0.26 and A260/A230 ranging between 1.74±0.23 to 2.98±0.22. This new method has been validated for other fruit crops like mango, guava and jamun which yields DNA amenable for PCR amplifications without use of any PCR enhancers.