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Karunakaran G.,Central Horticultural Experiment Station IIHR | Ravishankar H.,Central Horticultural Experiment Station IIHR | Ravishankar H.,Central Institute for Sub tropical Horticulture | Sakthivel T.,Central Horticultural Experiment Station IIHR | And 3 more authors.
Indian Journal of Horticulture | Year: 2014

In the present study, 45- and 60-day-old Coorg mandarin micro-buds were budded on five- and six-month-old Rangpur lime rootstock seedlings. Higher success of micro-budded plants was recorded (30.71%) on six-month-old Rangpur lime rootstocks by using 60-day-old scion buds, which was higher than the five-month-old rootstock seedlings (18.66%) with same age of scion buds. Further, in order to increase the success, microbudding was attempted under protected structures. Success of micro-budding on Rangpur lime was higher in polyhouse (56.49%) than shade nethouse (34.44%) and open conditions (21.50%) than other rootstocks. The number of days taken for sprouting was much earlier under protected conditions in Rangpur lime (13.36%) followed by Troyer citrange (14.82%) and trifoliate orange (19.54%) rootstocks. Micro-budded plants under open conditions took longer time for sprouting on Rangpur lime (18.32). Success of micro-budding was found to be significantly higher in protected structures than the open conditions for all the rootstocks. The results suggested that micro-budding technique could be gainfully exploited for shortening the propagation period and early biological indexing of citrus. © 2014, Horticulture Society of India. All rights reserved


Vishwakarma M.K.,Sam Higginbottom Institute of Agriculture, Technology and Sciences | Bajpai A.,Central Institute for Sub tropical Horticulture
Asian Journal of Plant Sciences | Year: 2012

With view to identify the causes of Malformation, Cyto-morphological studies were undertaken on malformation in relation to normal in two cultivars of (Mangifera indica L.) which is widely cultivated for its fruits. Malformation reduced the panicle length and its emergence as compared to normal; panicle length as well as number of flowers reached its optimum on 12 days in normal, while in malformed tissue, these two processes continued up to 33 days. Very high frequency of flowers emergence in malformed as compared to normal irrespective of the cultivars was also noted. The presence of high frequency of multicellular bulbous trichome in the malformed tissue of both the cultivars was probably noted for the first time. Further, meiotic cell division was found to be highly disturbed leading to many meiotic irregularities, such as, laggards, bridges, non orientation of spindles, cell cycle variations, variation in nucleolus number etc., in the malformed tissues. Meiotic abnormalities and presence of high frequency of multicellular bulbous trichome may be attributed to the reduction in growth of panicle as well as flowering pattern, resulting in poor fertility and fruit setting. © 2012 Asian Network for Scientific Information.


Mishra M.,Central Institute for Sub tropical Horticulture | Shree Y.,Central Institute for Sub tropical Horticulture | Pati R.,Central Institute for Sub tropical Horticulture | Seal S.,Central Institute for Sub tropical Horticulture | And 4 more authors.
Indian Journal of Genetics and Plant Breeding | Year: 2010

Nucellar embryogenesis was induced in Mangifera indica L. cv. Kurakkan, a polyembryonic salt tolerant, dwarfing rootstock. Nucellus tissue excised from 3.5 cm long fruits developed pro-embryonic callus in 19 days of inoculation on modified MS medium supplemented with 4.52μM 2,4-D, 0.05% malt extract and 13.78μM spermidine. Somatic embryogenesis exhibited high frequency (158.33 embryos). However, all the differentiated embryos proliferated on medium having low level of sucrose (4% w/v) and auxin (2.26μM 2,4-D). Most of the proembryonic calli converted into heart shaped and cotyledonary embryos by reducing temperature to 15°C. Somatic embryos were matured on modified MS medium fortified with 0.38μM ABA, 0.57μM IAA and 30.30μM PEG. Matured somatic embryos germinated (around 30%) on MS medium supplemented with 2.68μM NAA, 11.60μM kinetin and 2736.9μM glutamine.


Yadava L.P.,Central Institute for Sub tropical Horticulture
American Journal of Plant Physiology | Year: 2012

Various doses of paclobutrazol (12.5, 25, 50 and 100 ppm) and ethephon (100, 200, 400 and 800 ppm) on flowering, fruit characters and quality of cape gooseberry (Physalisperuviana L.) were observed. Results inhcated that ethephon 800 pprn delayed flowering but prolonged duration of fruit set and early ripening by 30.25, 2.5 and 10.13 days, respectively over control. 400 pprn of ethephon was more effective than 800 pprn in respect of fruit weight and quality constituents. In the same trend by 17.5, 2.37 and 5.85 days delay in flowering, prolonged duration of fruit set and early ripening, respectively were recorded with paclobutrazol 100 ppm. However, ethephon 400 pprn and paclobutrazol 50 pprn significantly influenced the fruit set, growth rate, cumulative growth, volume, density and TSSlacihty ratio of the fruit as compared to control and other concentrations of the retardants. © 2012 Academic Journals Inc.


Bhattacherjee A.K.,Central Institute for Sub Tropical Horticulture | Dikshit A.,Central Institute for Sub Tropical Horticulture | Kumar S.,Central Institute for Sub Tropical Horticulture | Tandon D.K.,Central Institute for Sub Tropical Horticulture
Indian Journal of Natural Products and Resources | Year: 2013

Aonla products are commercially prepared either from fresh fruits or from fruits stored in water/salt solution. In the present investigation aonla fruits were stored in water up to 21 days at room temperature and fruits were withdrawn at 7 days interval for the preparation of products namely candy and segments-in-syrup. The fruits and products were assessed for ascorbic acid and polyphenol contents besides sensory evaluation of the products. A gradual decrease in ascorbic acid (306 to 75 mg/100 g) and polyphenols (2.64 to 0.90%) in fruits was noticed during 21 days of storage. Almost 75% of ascorbic acid and 65% of polyphenols were lost during storage of fruits in water up to 21 days. The loss was also reflected in the products prepared from the stored fruits. The retention of ascorbic acid in candy and segments-in-syrup was around 30%, while that of polyphenols was around 60-70% when prepared from 21 days of fruit storage as compared to those prepared from fresh fruits. The organoleptic quality of the prepared products decreased with the increase in the storage period of fruits. Highly acceptable products could be prepared from fresh fruits and fruits stored in water up to 7 days without much loss in the contents of ascorbic acid and polyphenols. However, the products prepared from the fruits after 21 days of storage were poor in quality and unacceptable organoleptically. It could be concluded from the study that acceptable nutritious value added products could be prepared from fresh as well as fruits stored up to 7 days in water.

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