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Kumar A.,Sardar Patel University | Singh U.,ICAR Central Institute for Research on Cattle | Singh R.,Livestock Research Station | Vinoo R.,Livestock Research Station
Indian Journal of Animal Sciences

The study was undertaken to assess the genetic and non genetic factors affecting the productive and reproductive performance of Ongole cattle. Record (3,070) of calf weight and lactation records (2,900) of 729 Ongole cows, maintained at livestock farms (LRS, Lam, Guntur; LRS, Mahanandi; GCBF, Chadalwada; and GLF, Chintaldevi) in Andhra Pradesh, daughter of 67 bulls during 25 years from 1985 to 2009 were used in the analysis. The male calves were heavier than female calves. Low heritability estimated for lactation milk yield and reproductive traits indicated that there is less additive genetic variance in these traits and individual selection will not be helpful for improving them. Also, high genetic correlation between lactation milk yield and peak yield indicated that selection for peak yield may bring reasonable genetic improvement in milk yield of Ongole cows. The results also revealed there is a further scope for improvement in milk production and reproductive performance of the animals through better feeding and management practices. Source

Mahajan V.,Sher-e-Kashmir University of Agricultural Sciences and Technology | Das A.K.,Sher-e-Kashmir University of Agricultural Sciences and Technology | Das A.K.,ICAR Central Institute for Research on Cattle | Taggar R.K.,Sher-e-Kashmir University of Agricultural Sciences and Technology | And 2 more authors.
Indian Journal of Animal Sciences

In the present study, association of polymorphic variants of keratin-associated protein (KAP) 3.2 gene with wool traits in Rambouillet sheep was investigated. Genomic DNA was isolated from blood samples of 100 Rambouillet sheep. A 393 bp segment was amplified by PCR using ovine specific primers for KAP 3.2 gene. The genotyping was done by using PCR-SSCP. KAP 3.2 gene locus revealed 3 genotypes, viz. AA, AB and BB with genotype frequencies 0.46 (46), 0.40 (40) and 0.14 (14), respectively, with allele frequencies for allele A (0.66) and allele B (0.34). The result showed that the population was in Hardy-Weinberg equilibrium. Population genetic indexes such as expected heterozygosity (He) 0.45, effective number of alleles (ne) 1.81, Shannon indices (I) 0.64, Fixation index of individual with sub-population (FIS) 0.11, Standard error (SE) 0.03 and polymorphic information content (PIC) 35% were found. The least squares means of various genotypes for greasy fleece weight (GFW) differed significantly. The animals with AB genotype (2.08±0.08 kg) had higher GFW followed by AA genotype (1.75±0.08 kg), and BB genotypes (1.71±0.15 kg) was found to be associated. From the study, it may be concluded that KAP 3.2 gene might be a potential molecular marker for genetic selection in Rambouillet sheep. © 2015 Indian Journal of Animal Sciences. Source

Singh V.,P.A. College | Misra A.K.,P.A. College | Misra A.K.,Maharashtra Animal and Fishery Sciences University | Kumar S.,P.A. College | And 2 more authors.
Indian Journal of Animal Research

The objective of the present experiment was to investigate the effect of cysteamine and P-mercaptoethanol supplementation on in-vitro maturation, cleavage of oocytes and development of embryo in buffalo (Bubalus bubalis). Oocytes were aspirated from abattoir ovarian follicles of 3–10 mm diameter followed by maturation in the media in vitro containing cysteamine/p-mercaptoethanol (treatment) and without antioxidant (control). Matured oocytes were co-incubated with sperm (approx.1×106/ml) of Murrah bull in mSOF medium using heparin (10 μg/ml). After 22 h of oocyte-sperm incubation, fertilized oocytes were stripped of cumulus cells and cultured in mSOF medium for 8 days to study embryo development. The oocyte maturation rate improved significantly (P<0.05) following addition of 50 or 100 μM of cysteamine and 10, 50 and 100 μM of P-mercaptoethanol (ME), respectively as compared to control. The cleavage rate was found to be significantly (P<0.05) higher at 50 and 100 μM of cysteamine and at all concentrations ofb-mercaptoethanol as compared to control and development of embryos to morula stage was significantly (P<0.05) improved with 50 μM cysteamine/p-mercaptoethanol. © 2016, Agricultural Research Communication Centre. All rights reserved. Source

Mondal K.,P.A. College | Chakravarti S.,Indian Veterinary Research Institute | Ghosh A.K.,P.A. College | Kumar S.,Indian Veterinary Research Institute | And 5 more authors.
Molecular Biology Reports

Factor-XI deficiency (FXID) is inherited as autosomal lethal recessive disorder of carrier Holstein–Friesian bulls. A 76 base pair segment insertion into exon 12 in Factor-XI gene causes FXID in cattle. Keeping this in view the present study was conducted to screen breeding bulls of both indigenous and exotic breeds for mutation in Factor-XI gene and to find out the frequency of FXID carrier animals in breeding bulls. A total of 120 bulls of different age group maintained at Frozen Semen Bull Station, India were randomly selected from different cattle breeds to screen presence of FXID syndrome in breeding sires. Genomic DNA was isolated from blood of the selected bulls. PCR parameters were standardized to obtain 244 and 320 bp amplicons. The results showed that 2 Sahiwal bulls out of 120 animals were carrier for FXID. Amplicons of the carrier animals were sequenced and annoted, which confirms a 76 bp insertion in the exon 12. Bleeding and clotting time showed considerable discrepancy in the carrier animals as compared to the normal animals. The findings of relative mRNA expression of Factor XI transcript revealed identical tendency in the carrier. The frequency of carrier animals and mutant allele was 2.5 % and 0.025 respectively. This study recommends for screening of breeding at AI bull centers in the country for FXID. The study also stands a merit for identification of FXID carrier in Bos indicus for the first time. © 2016, Springer Science+Business Media Dordrecht. Source

Prasad J.K.,Indian Veterinary Research Institute | Ramteke S.S.,Indian Veterinary Research Institute | Perumal P.,National Research Center on Mithun | Ghosh S.K.,Indian Veterinary Research Institute | And 3 more authors.
Animal Reproduction Science

Buffalo spermatozoa are comparatively more susceptible to freezing hazards than cattle spermatozoa. In recent times incubation of spermatozoa with cholesterol-loaded-cyclodextrins (CLC) has shown improvements in semen quality in several species. Therefore, this study was undertaken to evaluate the incubation level of CLC at which maximum benefit is derived for the buffalo spermatozoa. For the study, 120 million spermatozoa were incubated in 2, 3 and 4mg/mL of CLC (Gr II, III and IV, respectively) and cholesterol and phospholipids content, their ratio, flow cytometric evaluation of plasma membrane integrity (PMI), plasma membrane fluidity and extent of cryoinjury (Chlortetracycline, CTC assay) were compared with an untreated control (Gr I). Additionally the ability of cholesterol-loaded-spermatozoa to undergo induced acrosome reaction (IAR) using ionophore calcium (A23187) was evaluated in frozen-thaw samples. Data show a significant and linear increase (CV=0.88) in cholesterol content of spermatozoa in Gr II, III and IV and a significant decrease in phospholipids content at frozen-thaw stage in Gr IV than Gr III spermatozoa. The study revealed a significant improvement in PMI and significant reduction in plasma membrane fluidity and cryoinjury of CLC treated spermatozoa at progressive stages in three groups compared to control. Nevertheless, spermatozoa of Gr II, III and IV were significantly less responsive to ionophore calcium (A23187) than Gr I. This study shows for the first time that incubation of buffalo bull spermatozoa with CLC (3mg/120×106) prior to processing permits greater numbers of sperm to survive cryopreservation while allowing spermatozoa to capacitate and the acrosome to react to AR inducer ionophore calcium (A23187). © 2015 Elsevier B.V. Source

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