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Kumar R.,University of Lucknow | Kushwaha B.,University of Lucknow | Nagpure N.S.,University of Lucknow | Behera B.K.,Central Inland Fisheries Research Institute CIFRI | Lakra W.S.,Central Institute of Fisheries Education

Genetic variability was assessed among three species of genus Channa, namely C. gachua, C. punctata and C. striata, using karyological tools including karyomorphology, silver nitrate and chromomycin A3 staining of nucleolar organizer regions (NORs) and C-banding. Additionally, sequence variation in partial 18S and divergent domain 1 of 28S ribosomal DNA was utilized for inter-species diversity assessment. Diploid chromosome numbers of 32, 40 and 52 in the three species were observed and the karyotype formula was derived as 18m + 12sm + 2st (fundamental arm number [FN] = 62); 6m + 2sm + 10st + 22t (FN = 48) and 12m + 10sm + 14st + 16t (FN = 86) in C. punctata, C. striata and C. gachua, respectively. Similarly, variations were also observed in number and location of silver and chromomycin A3 stained NORs and C-banding patterns. DNA sequencing generated sizes for 18S of 243, 248, 245 bp and for 28S of 401, 392 and 393 bp in C. gachua, C. punctata and C. striata, respectively. The results indicated that the cytogenetic and molecular markers are useful for the assessment of genetic diversity among the fish species and could provide valuable information for germplasm evaluation and ex situ conservation. © 2013 Copyright Dipartimento di Biologia Evoluzionistica, Università di Firenze. Source

Prusty A.K.,Project Directorate for Farming System Research PDFSR | Meena D.K.,Central Inland Fisheries Research Institute CIFRI | Mohapatra S.,Central Institute of Fisheries Education | Panikkar P.,Regional Center | And 3 more authors.
International Aquatic Research

As a new class of agricultural insecticides, synthetic pyrethroids are widely used to control insect pests. Synthetic pyrethroids have been shown to enter the aquatic environment from agricultural runoff or drift from aerial and ground-based spraying applications posing threat to fishes which are less tolerant to pesticides through direct exposure. These insecticides interfere with the sodium channel of the nervous system resulting in prolonged sodium tail current. Widespread application of these chemicals has warranted the attention of the ecologist to understand the impact of these chemicals on the aquatic environment. In this perspective, an updated account of toxicological evaluation of three type II synthetic pyrethroids, viz. deltamethrin, cypermethrin and fenvalerate in terms of their physico-chemical, metabolic, hematological, histological, behavioral and reproductive aspects with respect to the fishes has been presented which may be useful for policy makers, academics, environmental scientists and agricultural professionals needing ready access to this information. The aim of the present synoptic literature appraisal was to summarize the main effect of current use, type II synthetic pyrethroids (deltamethrin, cypermethrin and fenvalerate) on aquatic environment due to their persistence and accumulation. This article will focus on non-target organisms in inland fresh water environment with special reference to fin fishes and will critically evaluate the toxicity of these pyrethroids in terms of growth inhibition, metabolic disorders, neurotoxicity, reproductive failure, enzymatic dysfunction, haematological alterations, and tissue damages. The rationalized information in this milieu may be useful in ecological risk evaluation and human health management as fish serves as an important bio-indicator for aquatic systems health. © 2015, The Author(s). Source

Saha S.,Central Inland Fisheries Research Institute CIFRI | Hassan M.A.,Central Inland Fisheries Research Institute CIFRI | Sharma A.P.,Central Inland Fisheries Research Institute CIFRI
International Journal of Pharma and Bio Sciences

The effect of dietary supplementation of commercial probiotics Bifilac® on growth of rohu, Labeo rohita fingerlings were evaluated. Experimental diet (ED) was supplemented with Bifilac® (3g/kg diet). Reference diet (RD) was not supplemented with Bifilac®. The feeding trial was conducted using L. rohita fingerlings (5.30±0.01 g) for 60 days in triplicates. The groups D1 and D2 were fed daily with RD and ED, respectively, while the D3 group was fed alternately with RD and ED at an interval of 7 days. All experimental groups were fed ad libitum twice daily at 8:00 am and 2:00 pm. The growth performance and feed utilization efficiencies were highest for D2. Carcass protein content was found highest for D2. The digestive enzyme activity and the heterotrophic bacterial count of gut were highest for D2 fish group. So, daily dietary supplementation of Bifilac® resulted in improved growth and feed utilization efficiencies of L. rohita fingerlings. Source

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