Central Hospital of Zibo

Zibo, China

Central Hospital of Zibo

Zibo, China
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AIM: To study the nerve fiber layer thickness and other parameters of optic disc in patients with retinitis pigmentosa. METHODS: Totally 40 patients (80 eyes) with retinitis pigmentosa from April 2015 to April 2016 in our hospital were selected as the observation group and 40 healthy subjects as control group. All the subjects were examined by OCT. Disc parameters of retinal nerve fiber layer (RNFL) thickness, rim volume, cup volume, cup vertical diameter ratio, cup level diameter ratio, cup area ratio, rim area, cup area, disc area in temporal, superior, nasal and inferior quadrant were compared. RESULTS: The thickness of the whole circle, nasal, temporal, superior and inferior RNFL in the eyes of RP group was significantly higher than those in the normal control group, the difference was statistically significant (P<0.05); RP eyes group with rim volume, cup volume, cup vertical diameter ratio, cup level diameter ratio, cup area ratio, rim area, cup area, disc area parameters were significantly higher than those in normal control group, the difference was statistically significant (P<0.05). CONCLUSION: The mean thickness of RNFL in patients with retinitis pigmentosa at temporal, nasal, the whole circle compared with healthy subjects are thicker, while cup volume, cup vertical diameter ratio, cup level diameter ratio, cup area ratio, rim area and cup area increased. Copyright 2017 by the IJO Press.


Yang Y.,University of Sichuan | Zhang X.,Central Hospital of Zibo | Fu Y.,University of Sichuan | Yang H.,University of Sichuan
Toxicology Letters | Year: 2014

Lead is a toxic heavy metal with many recognized adverse health side effects. The central nervous system is the main target of lead toxicity. Although many studies on lead toxicity were conducted, the mechanism of lead toxicity remains uncertain. One possible attribution is the immature blood-brain barrier that causes lead exposure in children. Few studies have investigated the cytokine changes caused by this exposure. Novel cytokines were detected by RayBio® Human Cytokine Antibody Array and validated by enzyme-linked immunosorbent assay. Several children were admitted to West China Second University Hospital, after a serious lead pollution event in longchang, Sichuan, China. A total of 4 children with elevated blood lead levels (BLLs) and 4 children with low BLLs were randomly chosen in the discovery set, and 40 children with elevated BLLs and 40 children with low BLLs were included in the validation set. Leptin and interleukin-8 (IL-8) were identified to be significantly different between children with elevated and low BLLs via RayBio® Human Cytokine Antibody Array. In the validation set, IL-8 was higher in children with elevated BLLs [median(P25-P75), 117.69(52.31-233.63) pg/mL] than in children with low BLLs [median(P25-P75): 17.70(10.75-26.52) pg/mL] (p=0.000). Leptin was lower in children with elevated BLLs [median(P25-P75): 1658.23(1421.86-2606.55) pg/mL] than in children with low BLLs [median(P25-P75): 4168.68(3246.32-4744.94) pg/mL] (p=0.000). In children with low BLLs, leptin was higher in children with BLLs<3μg/dL (N=7) [median(P25-P75): 7220.86(4265.72-7555.15) pg/mL] than in children with BLL ≥ 3μg/dL (N=33) [median(P25-P75): 4103.86(3163.40-4678.34) pg/mL] (p=0.026); IL-8 was significantly different in children with BLL<4μg/dL (N=13) [median(P25-P75): 12.49(8.25-14.86) pg/mL] than in children with BLL≥4μg/dL (N=27) [median(P25-P75): 21.98(13.64-33.50) pg/mL] (p=0.013). The results defined specific changes in cytokine expressions to lead exposure, which can be used to explore the mechanism of lead toxicity and monitor lead exposure. © 2014 Elsevier Ireland Ltd.


Zhang Y.,Fudan University | Zhang P.,Fudan University | Wan X.,Fudan University | Su X.,Fudan University | And 5 more authors.
Biomedicine and Pharmacotherapy | Year: 2016

Long non-coding RNAs (lncRNA) have been reported as key regulators in the progression and metastasis of prostate cancer (PCa). In this study, we found that the expression levels of HCG11 in PCa tissues were significantly lower than those in non-tumor tissues in publically available databases and in human PCa samples. Our results showed the expression levels of HCG11 in patients with PCa were associated with the age, Lymph Node Status (LN status), preoperative PSA level, Gleason score, and biochemical recurrence (BCR). Kaplan-Meier analysis indicated that downregulation of HCG11 expression in tissues was associated with poor survival of PCa patients. GO and KEGG pathway analysis were applied to explore the potential roles of HCG11. Moreover, a HCG11 mediated ceRNA network was built using co-expression relationships of the differentially expressed mRNAs and miRNAs. We believed that this study will provide a potential new therapeutic and prognostic target for prostate cancer. © 2016


Zhang L.,Shanghai JiaoTong University | Tian W.,Shanghai JiaoTong University | Tian W.,Central Hospital of Zibo | Kim S.,Shanghai JiaoTong University | And 3 more authors.
Drug Design, Development and Therapy | Year: 2014

Background: Arsenic sulfde (As4S4), the main component of realgar, a traditional Chinese medicine, has shown antitumor effcacy in several tumor types, especially for acute promyelocytic leukemia. In this study, we aimed to explore the effcacy and mechanism of As4S4 in gastric cancer.Methods: The effect of As4S4 on cell proliferation and apoptosis of gastric cancer cells was investigated by MTT assay, 4′,6-diamidino-2-phenylindole (DAPI) staining, and annexin V–fuorescein isothiocyanate/propidium iodide staining using gastric cancer cell lines AGS (harboring wild-type p53) and MGC803 (harboring mutant p53) in vitro. The expression of apoptosis-related proteins was measured by Western blotting, real-time polymerase chain reaction, and immunohistochemistry analysis. Mouse xenograft models were established by inoculation with MGC803 cells, and the morphology and the proportion of apoptotic cells in tumor tissues were detected by hematoxylin and eosin staining and TdT-mediated dUTP nick end labeling (TUNEL) assay, respectively.Results: As4S4 inhibited the proliferation and induced apoptosis of AGS and MGC803 cells in a time- and dose-dependent manner. As4S4 upregulated the expression of Bax and MDM2 while downregulated the expression of Bcl-2. The expression of p53 increased signifcantly in the AGS cells but did not readily increase in the MGC803 cells, which harbored mutant p53. Pifthrin-α, a p53 inhibitor, blocked the modulation of As4S4 on AGS cells, but not on MGC803 cells. Using xenograft as a model, we showed that As4S4 suppressed tumor growth and induced apoptosis in vivo and that the expression of p53 increased accordingly.Conclusion: As4S4 is a potent cytotoxic agent for gastric cancer cells, as it induced apoptosis both in vitro and in vivo through a p53-dependent pathway. Our data indicate that As4S4 may have therapeutic potential in gastric cancer. © 2015 Zhang et al.


Li J.,Central Hospital of Zibo | Zhang L.M.,Central Hospital of Zibo | Huang X.,Central Hospital of Zibo
Journal of Biological Regulators and Homeostatic Agents | Year: 2017

Diabetes shows no obvious clinical symptoms in the early stage, which makes it difficult to be detected; however, as a common and frequently occurring disease, its morbidity increases with the improvement in the standard of living. Therefore, early detection, diagnosis and treatment of diabetes are of great importance. This study investigated the application values of urinary albumin (Alb), immunoglobulin G (IgG) and Beta-microglobulin (β2mG) in the diagnosis of diabetes. Seventy-four elderly patients with confirmed diabetes who were admitted to the Central Hospital of Zibo from May 2014 to May 2015 were taken as an observation group, and fifty normal healthy people were taken as a control group. Levels and positive detection rates of Alb, IgG and β2mG in the two groups were compared. The results showed that the levels of Alb, IgG and β2mG of the observation group were higher than those of the control group, and the positive detection rates of Alb, IgG and β2mG were 74.3%, 62.2% and 67.6%, respectively, which were higher than 6%, 16% and 12% in the control group; the differences were all statistically significant (P<0.05). The difference of the content of Alb, IgG and β2mG between patients with different disease courses was statistically significant (P<0.05). Therefore, Alb, IgG and β2mG levels are closely related to severity of diabetes, which provides a medical reference to the diagnosis and examination of diabetes and has important clinical values. Copyright © by BIOLIFE, s.a.s.


Liu L.,First Peoples Hospital of Jining | Liang Z.,First Peoples Hospital of Jining | Li Y.,Central Hospital of Zibo
Solid State Sciences | Year: 2012

A highly sensitive and selective small molecule detection platform has been developed using aptamers immobilized on electrode surface. In our system, two aptamers were used - one of which is for ATP recognition and the other is for signal produce. We designed two probes L1 (containing ATP aptamer and a part of hemin aptamer) and L2 (containing the complementary strand of ATP aptamer and the rest of hemin aptamer) and immobilized L1 on electrode surface. L2 was used to hybridize to L1 and form L1-L2 duplex which brought the two parts of hemin aptamer into close proximity. Then hemin can be captured by this duplex and detected by electrochemical methods. When we introduced ATP into the system, the ATP binding destroyed the duplex and L2 diffused into the solution. As a result, hemin cannot be captured to bring electrochemical signal. © 2012 Elsevier Masson SAS. All rights reserved.


Sun M.,Shandong University | Sun M.,Central Hospital of Zibo | Su X.,Shandong University | Ding B.,Shandong University | And 5 more authors.
Nanomedicine | Year: 2012

Curcumin (CUR), a bioactive component of turmeric, which is a commonly used spice and nutritional supplement, is isolated from the rhizomes of Curcuma longa Linn. (Zingiberaceae). In recent years, the potential pharmacological actions of CUR in inflammatory disorders, cardiovascular disease, cancer, Alzheimers disease and neurological disorders have been shown. However, the clinical application of CUR is severely limited by its main drawbacks such as instability, low solubility, poor bioavailability and rapid metabolism. Multifarious nanotechnology-based delivery approaches have been used to enhance the oral bioavailability, biological activity or tissue-targeting ability of CUR. This article reviews potential novel drug delivery systems for CUR including liposomes, polymeric nanoparticles, solid lipid nanoparticles, micelles, nanogels, nanosuspensions, nanoemulsions, complexes and dendrimer/dimer, which provide promising results for CUR to improve its biological activities. © 2012 Future Medicine Ltd.


Zhang Q.-H.,Central Hospital of Zibo | Dou H.-T.,Central Hospital of Zibo | Tang Y.-J.,Central Hospital of Zibo | Su S.,Central Hospital of Zibo | Liu P.-S.,Shandong University
Archives of Gynecology and Obstetrics | Year: 2015

Purpose: Ovarian cancer is among the top diseases in the list of malignant gynaecologic tumors. In the present study, we aim to investigate the effect of lentivirus-mediated knockdown of Krüppel-like factor 9 (KLF9) on cell viability and tumor growth in ovarian cancer. Methods: Firstly, the expression of KLF9 was determined by real-time PCR and western blot in human ovarian cancer tissues. Then, endogenous KLF9 expression was silenced by lentivirus in SKOV3 and OVCAR3 ovarian cancer cells, and followed by MTT and BrdU incorporation assays, cell cycle analysis and tumor xenografts in nude mice. Results: Our results found that the expression of KLF9 is up-regulated in human ovarian cancer. As expected, KLF9 knockdown significantly inhibited cell proliferation and resulted in cell cycle arrest in the G0/G1 phase. Besides, KLF9 deficiency significantly inhibited tumor growth in nude mice. Conclusion: Therefore, our data reveal that lentivirus-mediated KLF9 silencing might be promising in the treatment of human ovarian cancer. © 2014, Springer-Verlag Berlin Heidelberg.


Gao Y.,Shandong University | Wang C.,Shandong University | Sun M.,Central Hospital of Zibo | Wang X.,Shandong Academy of Sciences | And 3 more authors.
Journal of Biomedical Nanotechnology | Year: 2012

The current study was aimed at preparing the curcumin nanosuspension (CUR-NS) to improve the solubility and oral absorption of CUR. Prepared from a tandem of ultra-turrax homogenization and high pressure homogenization, the CUR-NS showed spherical shape under transmission electron microscopy with an average diameter of 210.2 nm. The absorption of CUR-NS in the gastrointestinal (GI) tract was studied in rats using an in situ single pass perfusion method. It was found that the absorption percentage in stomach was 9.20% within 2 h. The absorption process in intestine was first-process with passive diffusion mechanism, and the main absorptive segments were proven to be in the duodenum and jejunum. A pharmacokinetic study was conducted in mice after oral administration of CUR at 250 mg/kg in the form of either CUR-NS or CUR suspension. The plasma concentration-time curves were both fitted to a one-compartment model and the relative bioavailability of CUR-NS to CUR suspension was 680.03%. The effects of CUR-NS on the structural properties of the intestinal mucosal membrane of rats were investigated by fluorescence polarization and circular dichroism in vitro. After treatment with CUR-NS, not only the fluidity of intestinal mucosal membrane increased but also the conformation of the membrane protein loosed, which increased the gastrointestinal absorption of CUR-NS. These studies provided the evidence that NS was valuable as an oral delivery carrier to enhance the absorption of CUR. Copyright © 2012 American Scientific Publishers All rights reserved.


Liu W.-F.,Huazhong University of Science and Technology | Zuo H.-J.,Huazhong University of Science and Technology | Chai B.-L.,Huazhong University of Science and Technology | Peng D.,Huazhong University of Science and Technology | And 5 more authors.
International Journal of Biochemistry and Cell Biology | Year: 2011

Tetraspanin CD151 mainly associates with laminin-binding integrins and forms CD151-integrin complex. We previously reported that CD151 could be a potential target for angiogenesis, but the mechanisms involved are still unclear. This study investigated the role of CD151-integrin complex in angiogenesis and the signaling mechanisms involved. Here we showed that CD151 and CD151-AAA mutant were both well expressed at the protein level. CD151 gene transfer promoted angiogenesis and improved skin temperature of the lateral ischemic hindlimb, whereas CD151-AAA mutant abrogated the increase in capillary density and skin temperature. Further, CD151-AAA mutant failed to activate the FAK, ERK, PI3K/Akt/eNOS, and Rac1/Cdc42 signaling pathways. Moreover, CD151-AAA mutant was unavailable to promote bovine aortic endothelial cells (BAECs) proliferation and migration, in contrast to the effects of CD151. The results suggested that formation of CD151-integrin complex was likely to be a prerequisite for CD151-induced angiogenesis and signaling pathways. © 2011 Elsevier Ltd. All rights reserved.

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