Li Y.,Central Hospital of Xinxiang |
Li J.-L.,Central Hospital of Xinxiang |
Zhai H.-P.,the Second Peoples Hospital of Xinxiang |
Chang F.-L.,Central Hospital of Xinxiang |
And 2 more authors.
Chinese Journal of Medical Imaging Technology | Year: 2014
Objective: To investigate the application value of echocardiography in the prenatal diagnosis of fetal simple ventricular septal defect (VSD) and the follow-up. Methods: Totally 35 fetuses with suspected VSD by prenatal echocardiography were enrolled. The fetus echocardiograph features were analyzed and the cases were followed before and after birth to observe the size of defect and the condition of closure. Results: Two fetuses were misdiagnosed. Twenty-six were non-high-risk fetuses and the other 7 were high-risk ones; 18 fetuses (18/33, 54.55%) were muscular ventricular septal defect, 13 fetuses (13/33, 39.39%) were perimembranous ventricular septal defect, 1 fetus (1/33, 3.03%) was stem-inferior ventricular septal defect, and 1 fetus (1/33, 3.03%) was sub-ralvular ventricular septal defect. One case was lost for follow-up. Two cases were naturally closed in the womb, 21 cases recovered from the defect in the three years after birth, 9 cases failed to close. The incidence of defect closure in the non-high-risk fetuses was higher than that in the high-risk fetuses (P<0.05). The incidence of muscular ventricular septal defect closure was apparently higher that of perimembranous ventricular septal defect and the other defects (all P<0.05). The closure incidence of the defect with diameter of ≤3.0 mm was apparently higher compared with the defects with diameter of 3.1-5.0 mm and >5.0 mm (all P<0.05). Conclusion: Echocardiography has an important clinical value on prenatal diagnosis, follow-up observation and chooses of treatment in simple VSD. Copyright © 2014 by the Press of Chinese Journal of Medical Imaging and Technology.
Fan W.,Central Hospital of Xinxiang |
Wang W.,Central Hospital of Xinxiang |
Wu J.,Central Hospital of Xinxiang |
Ma L.,Central Hospital of Xinxiang |
Guo J.,Zhengzhou University
Biomarkers in Medicine | Year: 2017
Aim: This study aimed to identify CD4+ T-cell-derived microparticles (MPs) and investigate their roles in rheumatoid arthritis (RA). Methods: Synovial fluids from 34 RA, 33 osteoarthritis patients and 42 healthy individuals were analyzed by flow cytometry. Human fibroblast-like synoviocytes and peripheral blood mononuclear cells were cultured with or without isolated MPs, chemokines and cytokines were measured by ELISA. Results: CD4+CD161+CD39+ and CD4+CD39+CD73+ MPs were abundantly present in RA patients, which were positively or negatively correlated with RA features, respectively. Chemokines CCL20, CCL17 and CCL22, and cytokines IL-17 and IL-10 were influenced by these MPs in human fibroblast-like synoviocytes (HFLS) or PMBCs. Conclusion: CD4+ T-cell-derived CD161+CD39+ and CD39+CD73+ MPs could serve as new reciprocal biomarkers for RA evaluation. © 2017 Future Medicine Ltd.
Wei W.,Central Hospital of Xinxiang |
Yang Y.,No150 Central Hospital Of Pla |
Yang Y.,Shanghai University |
Cai J.,No150 Central Hospital Of Pla |
And 5 more authors.
Cellular Physiology and Biochemistry | Year: 2016
Aims: MicroRNAs (miRNAs) are dysregulated in a wide range of malignant diseases, confirming their crucial role in tumor metastasis. MiRNA-30a-5p, a member of the miR-30 family, has been implicated in many types of cancers, including colorectal cancer, a leading cause of death worldwide. Methods: qRT-PCR, Western blot, Transwell assay,luciferase reporter assay were performed in the present study. Results: In this study, miR-30a-5p was found to be significantly downregulated in human colorectal cancer tissue specimens and cell lines compared with non-cancerous tissues and cells. The overexpression of miR-30a-5p inhibited the migratory and invasive abilities of colorectal cancer cells and suppressed the epithelial-mesenchymal transition, a crucial process in metastasis. Bioinformatic algorithms and luciferase reporter assays revealed that integrin β3 (ITGB3) is a direct target of miR-30a-5p. Importantly, overexpression of ITGB3 in colorectal cancer cells rescued these cells from miR-30a-5p-mediated suppression of metastasis and restored the epithelial-mesenchymal transition. Conclusion: Taken together, our study provides the first evidence that miR-30a-5p suppresses colon cancer metastasis through the inhibition of ITGB3. Thus, targeting miR-30a-5p might serve as a promising therapeutic strategy for the treatment of colorectal cancer. © 2016 The Author(s) Published by S. Karger AG, Basel.
PubMed | Xinjiang Medical University, The First Peoples Hospital of Shangqiu, Shantou University, Zhengzhou University and 43 more.
Type: Journal Article | Journal: Nature genetics | Year: 2014
We conducted a joint (pooled) analysis of three genome-wide association studies (GWAS) of esophageal squamous cell carcinoma (ESCC) in individuals of Chinese ancestry (5,337 ESCC cases and 5,787 controls) with 9,654 ESCC cases and 10,058 controls for follow-up. In a logistic regression model adjusted for age, sex, study and two eigenvectors, two new loci achieved genome-wide significance, marked by rs7447927 at 5q31.2 (per-allele odds ratio (OR) = 0.85, 95% confidence interval (CI) = 0.82-0.88; P = 7.72 10(-20)) and rs1642764 at 17p13.1 (per-allele OR = 0.88, 95% CI = 0.85-0.91; P = 3.10 10(-13)). rs7447927 is a synonymous SNP in TMEM173, and rs1642764 is an intronic SNP in ATP1B2, near TP53. Furthermore, a locus in the HLA class II region at 6p21.32 (rs35597309) achieved genome-wide significance in the two populations at highest risk for ESSC (OR = 1.33, 95% CI = 1.22-1.46; P = 1.99 10(-10)). Our joint analysis identifies new ESCC susceptibility loci overall as well as a new locus unique to the population in the Taihang Mountain region at high risk of ESCC.
Wang G.,Henan Normal University |
Li B.,Central Hospital of Xinxiang |
Hao Y.,Henan Normal University |
Hao Y.,Key Laboratory for Cell Differentiation Regulation |
And 6 more authors.
Cell Biology International | Year: 2013
To explore the relevance of non-alcoholic fatty liver disease (NAFLD) to liver regeneration (LR), rat models of non-alcoholic steatohepatitis (NASH) and LR were established, respectively, then Rat Genome 230 2.0 Array was used to detect the gene expression abundance of them, and the reliabilities of the array data were confirmed by real-time RT-PCR. As a result, the expression of 93 genes was significantly changed during NAFLD occurrence and 948 genes in LR. Hierarchical clustering indicated that the expression profiles of the above two events were quite different. K-means cluster classified their expression patterns into four clusters, and gene expression trends of clusters 1, 2 were similar in NAFLD and LR, while clusters 3, 4 were contrary with the gene expression changes of LR more abundant. DAVID classifications and functional enrichment analysis found that lipid metabolism and carbohydrate metabolism were stronger in NAFLD than in LR, but some other physiological activities including inflammation/immune response, cell adhesion, and migration, cell proliferation and differentiation in NAFLD were weaker than in LR. IPA further indicated that lipid metabolism, inflammation response, and cellular development were highly associated with NAFLD, and thus identified some potential biomarkers for NAFLD. © 2013 International Federation for Cell Biology.