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Wei W.,Central Hospital of Xinxiang | Yang Y.,No150 Central Hospital Of Pla | Yang Y.,Shanghai University | Cai J.,No150 Central Hospital Of Pla | And 5 more authors.
Cellular Physiology and Biochemistry | Year: 2016

Aims: MicroRNAs (miRNAs) are dysregulated in a wide range of malignant diseases, confirming their crucial role in tumor metastasis. MiRNA-30a-5p, a member of the miR-30 family, has been implicated in many types of cancers, including colorectal cancer, a leading cause of death worldwide. Methods: qRT-PCR, Western blot, Transwell assay,luciferase reporter assay were performed in the present study. Results: In this study, miR-30a-5p was found to be significantly downregulated in human colorectal cancer tissue specimens and cell lines compared with non-cancerous tissues and cells. The overexpression of miR-30a-5p inhibited the migratory and invasive abilities of colorectal cancer cells and suppressed the epithelial-mesenchymal transition, a crucial process in metastasis. Bioinformatic algorithms and luciferase reporter assays revealed that integrin β3 (ITGB3) is a direct target of miR-30a-5p. Importantly, overexpression of ITGB3 in colorectal cancer cells rescued these cells from miR-30a-5p-mediated suppression of metastasis and restored the epithelial-mesenchymal transition. Conclusion: Taken together, our study provides the first evidence that miR-30a-5p suppresses colon cancer metastasis through the inhibition of ITGB3. Thus, targeting miR-30a-5p might serve as a promising therapeutic strategy for the treatment of colorectal cancer. © 2016 The Author(s) Published by S. Karger AG, Basel.


Wu C.,Peking Union Medical College | Wang Z.,U.S. National Institutes of Health | Wang Z.,SAIC | Song X.,Xinxiang Medical University | And 165 more authors.
Nature Genetics | Year: 2014

We conducted a joint (pooled) analysis of three genome-wide association studies (GWAS) of esophageal squamous cell carcinoma (ESCC) in individuals of Chinese ancestry (5,337 ESCC cases and 5,787 controls) with 9,654 ESCC cases and 10,058 controls for follow-up. In a logistic regression model adjusted for age, sex, study and two eigenvectors, two new loci achieved genome-wide significance, marked by rs7447927 at 5q31.2 (per-allele odds ratio (OR) = 0.85, 95% confidence interval (CI) = 0.82-0.88; P = 7.72 × 10-20) and rs1642764 at 17p13.1 (per-allele OR = 0.88, 95% CI = 0.85-0.91; P = 3.10 × 10-13). rs7447927 is a synonymous SNP in TMEM173, and rs1642764 is an intronic SNP in ATP1B2, near TP53. Furthermore, a locus in the HLA class II region at 6p21.32 (rs35597309) achieved genome-wide significance in the two populations at highest risk for ESSC (OR = 1.33, 95% CI = 1.22-1.46; P = 1.99 × 10-10). Our joint analysis identifies new ESCC susceptibility loci overall as well as a new locus unique to the population in the Taihang Mountain region at high risk of ESCC. © 2014 Nature America, Inc. All rights reserved.


PubMed | Xinjiang Medical University, The First Peoples Hospital of Shangqiu, Shantou University, Zhengzhou University and 43 more.
Type: Journal Article | Journal: Nature genetics | Year: 2014

We conducted a joint (pooled) analysis of three genome-wide association studies (GWAS) of esophageal squamous cell carcinoma (ESCC) in individuals of Chinese ancestry (5,337 ESCC cases and 5,787 controls) with 9,654 ESCC cases and 10,058 controls for follow-up. In a logistic regression model adjusted for age, sex, study and two eigenvectors, two new loci achieved genome-wide significance, marked by rs7447927 at 5q31.2 (per-allele odds ratio (OR) = 0.85, 95% confidence interval (CI) = 0.82-0.88; P = 7.72 10(-20)) and rs1642764 at 17p13.1 (per-allele OR = 0.88, 95% CI = 0.85-0.91; P = 3.10 10(-13)). rs7447927 is a synonymous SNP in TMEM173, and rs1642764 is an intronic SNP in ATP1B2, near TP53. Furthermore, a locus in the HLA class II region at 6p21.32 (rs35597309) achieved genome-wide significance in the two populations at highest risk for ESSC (OR = 1.33, 95% CI = 1.22-1.46; P = 1.99 10(-10)). Our joint analysis identifies new ESCC susceptibility loci overall as well as a new locus unique to the population in the Taihang Mountain region at high risk of ESCC.


He Z.-Y.,Sun Yat Sen University | Chi F.,Sun Yat Sen University | Li F.-Y.,Sun Yat Sen University | Wang J.-J.,Central Hospital of Xinxiang | And 2 more authors.
Chinese Journal of Cancer Prevention and Treatment | Year: 2011

OBJECTIVE: To evaluate the dosimetry advantages of moderate deep inspiration breath hold (mDIBH) achieved using an active breathing control (ABC) device in the target of forward whole-breast intensity-modulated radiotherapy after breast conserving surgery in breast cancer. METHODS: 18 patients after breast conserving surgery had received primary CT-simulation assisted by ABC to get two sets of CT image on the two breathing conditions which included one set from free breath (FB), and the other set from mDIBH. Forward whole-breast intensity-modulated radiotherapy plans were carried out in each set of CT image using Pinnacle7. 4 f planning systems and the differences of two plans had been compared. RESULTS: The coverage index and habituation index of the plans in FB were similar with those in mDIBH (1. 08±0. 01) vs (1. 09±0. 01), P = 0. 776; 0. 59±0. 08 vs 0. 60±0. 04, P = 0.821). The V 20 and V 30 of Ipsi-lateral lung in FB were higher than those in mDIBH (13. 07± 3. 93)% vs (9. 75±3. 58)%, (11. 68±3. 90)% vs (8. 12± 3. 23)%, P = 0.000; In the left breast cancer, The V 20 and V 30 of heart in FB were higher than those in mDIBH (5. 42±3. 21)% vs (2. 73±1.95)%, (3. 70±2. 84)% vs (1. 48±0. 22)%, P<0. 05. CONCLUSION: Compared with FB, mDIBH achieved using an ABC device significantly reduces heart and lung doses in the target of forward whole-breast intensity-modulated radiotherapy after breast conserving surgery in breast cancer.


Wang G.,Henan Normal University | Li B.,Central Hospital of Xinxiang | Hao Y.,Henan Normal University | Hao Y.,Key Laboratory for Cell Differentiation Regulation | And 6 more authors.
Cell Biology International | Year: 2013

To explore the relevance of non-alcoholic fatty liver disease (NAFLD) to liver regeneration (LR), rat models of non-alcoholic steatohepatitis (NASH) and LR were established, respectively, then Rat Genome 230 2.0 Array was used to detect the gene expression abundance of them, and the reliabilities of the array data were confirmed by real-time RT-PCR. As a result, the expression of 93 genes was significantly changed during NAFLD occurrence and 948 genes in LR. Hierarchical clustering indicated that the expression profiles of the above two events were quite different. K-means cluster classified their expression patterns into four clusters, and gene expression trends of clusters 1, 2 were similar in NAFLD and LR, while clusters 3, 4 were contrary with the gene expression changes of LR more abundant. DAVID classifications and functional enrichment analysis found that lipid metabolism and carbohydrate metabolism were stronger in NAFLD than in LR, but some other physiological activities including inflammation/immune response, cell adhesion, and migration, cell proliferation and differentiation in NAFLD were weaker than in LR. IPA further indicated that lipid metabolism, inflammation response, and cellular development were highly associated with NAFLD, and thus identified some potential biomarkers for NAFLD. © 2013 International Federation for Cell Biology.

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