Kusters M.,Central Hospital of the German Federal Armed Forces Koblenz |
Beyer S.,Central Hospital of the German Federal Armed Forces Koblenz |
Kutscher S.,Central Hospital of the German Federal Armed Forces Koblenz |
Schlesinger H.,Central Hospital of the German Federal Armed Forces Koblenz |
Gerhartz M.,Central Institute of the German Federal Armed Forces Koblenz
Journal of Pharmaceutical Analysis | Year: 2013
A rapid and simple method for the determination of polyhexamethylene biguanide (polyhexanide, PHMB) in liquid and gel-like pharmaceutical formulations by means of high performance liquid chromatography coupled to diode-array detection (HPLC-DAD) was developed. Best separation was achieved using a cyanopropyl bonded phase (Agilent Zorbax Eclipse XDB-CN column 4.6 mm×75 mm with particle size of 3.5 μm) as well as gradient elution consisting of acetonitrile/deionized water at a flow rate of 1.0 mL/min. The optimized and applied chromatographic conditions permitted separation of polyhexanide from interacting matrix with subsequent detection at a wavelength of 235 nm with good sensitivity. The method validation was carried out with regard to the guidelines for analytical procedures demanded by the International Conference on Harmonisation (ICH). Mean recoveries of 102% and 101% for gel-like as well as liquid preparations were obtained. Suitable repeatability as well as intermediate precision could be achieved with limits of detection ≤0.004 mg/mL for both formulations, equivalent to ≤0.004% PHMB concerning sample preparation. Determination of PHMB was accomplished without tedious sample preparation. Interacting matrix could be eliminated by the chromatographic procedure with excellent performance of system suitability. All analytical requirements were fulfilled permitting a reliable and precise determination of PHMB in pharmaceuticals. Furthermore, the developed method was applied to stability testing of pharmaceutical preparations containing PHMB. © 2013 Xi'an Jiaotong University.
Gallitzendorfer R.,Central Institute of the German Federal Armed Forces Medical Service Koblenz andernacher Strasse 100 |
Gallitzendorfer R.,Zentrales Institute des Sanitatsdienstes der Bundeswehr Koblenz |
Timm T.,Central Institute of the German Federal Armed Forces Medical Service Koblenz andernacher Strasse 100 |
Koch D.,Central Institute of the German Federal Armed Forces Medical Service Koblenz andernacher Strasse 100 |
And 2 more authors.
Chromatographia | Year: 2011
A liquid chromatographic method (LC) with diode array detection (DAD) for the routine screening and quantification of highly applicated polar herbicides in drinking water samples was developed. The investigated herbicides consisted of 12 sulfonylurea herbicides (amidosulfuron, flazasulfuron, foramsulfuron, iodosulfuron-methyl Na, mesosulfuron-methyl, metsulfuron-methyl, nicosulfuron, prosulfuron, thifensulfuron-methyl, triasulfuron and tritosulfuron) together with 6 polar pesticides of relevance (atrazine, desethylatrazine, desisopropylatrazine, chlortoluron, diuron, fluoxypyr). The herbicides were extracted and concentrated by off-line solid-phase extraction and subsequently eluates were analyzed by LC-DAD. Recoveries obtained from fortified water samples at 100 ng L-1 were in the range of 84-107% with RSD's <20%. The limit of detection varied from 2 to 16 ng L-1. © 2011 Springer-Verlag.