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Peng H.,Nanchang University | Peng H.,Peking Union Medical College | Yuan X.,Peking Union Medical College | Luo S.,Nanchang University | And 4 more authors.
European Journal of Pharmacology | Year: 2014

Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) can selectively induce apoptosis in cancer cells and is verified to be effective in various cancers. However, a variety of cancer cells are found to be resistant to TRAIL and the mechanisms are largely unknown. Moreover, multidrug resistance to traditional chemotherapeutic agents still remains a tough problem in clinical practice. Fortunately, our previous work proved the ability of PH II-7 in overcoming MDR phenotype through reactive oxygen species production in K562 and its MDR counterpart K562/A02 cells. Additionally, we further explored its potential in augmenting TRAIL induced apoptosis in cancer cells with various tissue origins. Our results showed PH II-7 up-regulated DR4/DR5 expression and augment TRAIL cytotoxicity through reactive oxygen species production, which provide a solid foundation for TRAIL in combination with PH II-7 in future clinical application. © 2014 Elsevier B.V. All rights reserved.

Han W.,Central hospital of Karamay
Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics | Year: 2011

To study the prognosis of childhood asthma and the factors influencing asthmatic attacks and prognosis. The medical data of 212 children with asthma who were followed up for more than 5 years were retrospectively studied. During the 5-year follow up, asthmatic attacks termination was found in 121 cases (57.1%) and asthma persistence was observed in 91 cases. Respiratory tract infections were found as the major factors inducing asthmatic attacks (71.7%), followed by inhaled allergens (17.0%).The children with asthma induced by respiratory tract infections had a higher remission rate of asthmatic attacks (61.2%) than those induced by allergens (41.7%) or exercises (26.3%). Three risk factors for asthma persistence were identified: concurrent allergic rhinitis and eczema, parental asthma and allergy-induced wheezing. The 5-year follow-up study demonstrated that asthmatic attacks stopped in the majority of children with asthma. Respiratory tract infections may be the major factors inducing acute asthma attacks. The children with asthma induced by respiratory infections may experience a better outcome. Atopic children or children with the genetic background of atopy are at high risks for the development of persistent asthma.

Huang Q.-X.,Xinjiang Medical University | Gu J.-P.,Xinjiang Medical University | Ji W.-Z.,Xinjiang Medical University | Bawudun D.,Xinjiang Medical University | And 3 more authors.
Chinese Journal of Interventional Imaging and Therapy | Year: 2012

Objective: To assess the impact factors on survival time of patients with advanced esophageal cancer after stent implantation. Methods: Data of 103 patients with advanced esophageal cancer who received covered endoscophageal stent implantation were retrospectively analyzed. Cox regression analysis was performed to examine the following factors on survival time, such as age, gender, fistula, tumor length, tumor invasion depth, pathologic type, grade of cell differentiation, clinical tumor stage, postoperative chemotherapy and radiotherapy. Results: Single factor Cox regression analysis showed that fistula, tumor length, tumor invasion depth, grade of cell differentiation and clinical tumor stage had significant influence on prognosis (all P<0.05). Multiple Cox regression analysis showed that only grade of cell differentiation and clinical tumor stage were independent influence factors on prognosis (both P<0.05). Conclusion: Grade of cell differentiation and clinical tumor stage are the major impact factors of prognosis that affect the survival of patients with unresectable esophageal carcinoma after stenting. There is no benefit for survivals treated with radiotherapy and/or chemotherapy after esophageal stenting.

Xu De M.,U.S. Center for Disease Control and Prevention | Li X.F.,U.S. Center for Disease Control and Prevention | Goan D.,University of North Texas Health Science Center | Yang De M.,U.S. Center for Disease Control and Prevention | And 4 more authors.
Biomedical and Environmental Sciences | Year: 2016

The objective of this study was to examine the prevalence of hypertension and identify its contributory factors in the labor force population in Karamay. A total of 2819 adults (55.9% male adults) were interviewed and examined. The overall crude prevalence of hypertension was 32.4%. Among 914 hypertensive patients, 34.8% were aware of their diagnosis, 22.1% received treatment, and 5.6% achieved blood pressure control. Hypertension was significantly correlated with age, overweight/obesity, central obesity, diabetes, and dyslipidemia in both men and women. In addition, less education, alcohol consumption, and less walking were risk factors for men. Effective hypertension prevention and control programs are urgently needed to decrease the burden of hypertension in this region. © 2016 The Editorial Board of Biomedical and Environmental Sciences.

Fan D.,Chinese Academy of Sciences | Li Z.,Chinese Academy of Sciences | Zhang X.,Chinese Academy of Sciences | Yang Y.,Tianjin Medical University | And 4 more authors.
Journal of Hematology and Oncology | Year: 2015

Background: Leukemic stem cells (LSCs) are frequently seen as a cause of treatment failure and relapse in patients with acute myeloid leukemia (AML). Thus, successful new therapeutic strategies for the treatment of AML should aim at eradicating LSCs. The identification of targets on the cell surface of LSCs is getting more and more attention. Among these, CD123, also known as the interleukin-3 (IL3)-receptor α chain, has been identified as a potential immunotherapeutic target due to its overexpression on LSCs in AML as well as on AML blasts, rather than normal hematopoietic stem cells. Methods: We constructed a CD123-targeted fusion protein antiCD3Fv-δIL3, with one binding site for T cell antigen receptor (TCRCD3) and the other for CD123, by recombinant gene-engineering technology. Cysteine residues were introduced into the V domains of the antiCD3Fv segment to enhance its stability by locking the two chains of Fv together with disulfide covalent bonds. The stability and cytotoxicity of the two fusion proteins were detected in vitro and in vivo. Results: Both fusion proteins were produced and purified from Escherichia coli 16C9 cells with excellent yields in fully active forms. High-binding capability was observed between these two fusion proteins and human IL3R, leading to the specific lysis of CD123-expressing cell lines KG1a; also, mononuclear cells from primary AML patients were inhibited in a colony forming assay in vitro, presumably by redirecting T lymphocytes in vitro. In addition, they displayed an antileukemic activity against KG1a xenografts in non-obese diabetic/severe combined immunodeficient (NOD/SCID) mice, especially disulfide-stabilized (ds)-antiCD3Fv-δIL3 for its improved stability. Conclusions: These results suggest that both fusion proteins display the antileukemic activity against CD123-expressing cell lines as well as leukemic progenitors in vitro and in vivo, especially ds-antiCD3Fv-δIL3. They could be the promising candidates for future immunotherapy of AML. © 2015 Dongmei et al.; licensee BioMed Central.

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