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Wang W.X.,Central Hospital of Huzhou
Zhonghua yi xue za zhi | Year: 2010

OBJECTIVE: To observe the clinical results of different concentration NaCl solution in hemorrhagic shock resuscitation. METHODS: The 75 cases with hemorrhagic shock were divided into five groups by means of random number table. Treat the cases with 0.9% (control group), 3%, 4.5%, 6% and 7.5% NaCl solution, respectively. Observe the changes of BP, HR, blood gas, renal function, electrolyte concentration of plasma, before and after treatment. RESULTS: After transfusion, compared with groups of 0.9% NS, the mean BP of patients in other four groups rise more quickly but only the group of 4.5% can last 30 minutes (P < 0.05), and K(+) of plasma in groups of 6%, 7.5% NS decline more obviously (P < 0.05), the group of 4.5% maintain the best lever. CONCLUSION: The mean BP of patients rise quickly and continue for a long time, in the group of 4.5%. Meanwhile the internal environment of the body maintain relatively stable in the group of 4.5%.

Qiu J.,Central Hospital of Huzhou | Fu Y.-F.,Zhejiang University | Zhou C.-Y.,Zhejiang University | Lu W.-G.,Zhejiang University
Chinese Journal of Cancer Prevention and Treatment | Year: 2015

OBJECTIVE: To investigate the expressions of CK2β and p53 in epithelial ovarian cancer tissues and to analysis their correlation with clinical pathological and survival significance. METHODS: 108 patients with epithelial ovarian cancer, 20 patients with benign epithelial ovarian tumor and 20 patients with bordline epithelial ovarian tumor who underwent treatment in Women's Hospital, School of Medicine, Zhejiang University between January 1, 2003 and December 31, 2005 were retrospectively analyzed. Immunohistoehemistry was used to assess the expressions of CK2β and p53. The differences among different epithelial ovarian tumor and the relationships between expression of CK2β, p53 and prognosis in epithelial ovarian cancer were analyzed. RESULTS: The positive expression rate of CK2β and p53 in epithelial ovarian cancer, bordline ovarian tumor and benign ovarian tumor was 63.0%(68/108) and 53.7%(58/108), 30.0%(6/20) and 20.0%(4/20), 0(0/20) and 0(0/20) respectively. The expression intensities of CK2β and p53 in epithelial ovarian cancer were signifieanly higher than that in benign(P<0.001, P<0.001)and bordline epithelial ovarian tumor tissues(P=0.003, P=0.006), and there was significant correlation between CK2β and p53 expression(r=0.377, P<0.001). The expression of CK2β protein in epithelial ovarian cancer was associated with tumor stage(P=0.001), CA125 level (P<0.001) and chemosensitivity(P=0.012); whereas the expression of p53 protein in epithelial ovarian cancer was correlated with histological grade (P=0.027) and CA125 level(P=0.027). Patients with positive expression of CK2β and p53 demonstrated a significantly worse prognosis than that of patients with negative expression of CK2β and p53 for disease-free and overall survival(all P<0.05). Multivariate survival analysis revealed that tumor stage (P<0.001) and positive p53 expression (P=0.018) provided significant independent prognostic value for disease-free survival, whereas tumor stage (P=0.001), CA125 level (P=0.040) and p53 expression (P=0.041) provided significant independent prognostic value for overall survival. CONCLUSIONS: The expressions of CK2β and p53 protein were significantly associated with the development and progression of epithelial ovarian cancer. p53 expression has important predictive value in predicting ovarian cancer survival. ©, 2015, The Editorial Board of Chinese Journal of Cancer Prevention and Treatment. All right reserved.

Lu P.,Central Hospital of Huzhou | Zhang G.,Zhejiang Cancer Hospital
Chinese Journal of Cancer Prevention and Treatment | Year: 2011

OBJECTIVE: To investigate the optimal methods of fluorescence in situ hybrdization in HER-2 gene detection. METHOD: Totally 55 samples of breast cancer were gathered by bath and direct digestion, dimethylformamide and denaturation law, were compared by detecting the success expression rate of HER-2 gene. RESULTS: In two digest methods, the success rates of hybridization were 86.21% (25/29) and 88.46% (23/26), respectively and with no difference (P>0.05). But hybridization signal ratio were 17.24% (5/29) and 46.15% (12/26), with significant difference (P<0.05). In two digest methods, the success rates were 73.08% (19/26) and 93.10% (27/29), with significant difference (P<0.05). CONCLUSION: Detecting HER-2 gene by fluorescence in situ hybridization, bath and denaturation law hybridization variability have higher success rate.

Mao H.,Zhejiang University | Gao W.,Central Hospital of Huzhou | Lu G.,First Peoples Hospital of Tonglu | Fang F.,First Peoples Hospital of Tonglu | Teng L.,Zhejiang University
Clinical Laboratory | Year: 2013

Background: To investigate the correlation of Arg-1 expression with clinicopathologic factors and prognosis of HCC patients, including recurrence and survival rate. Methods: We examined the expression of Arg-1 in HCC by immunohistochemistry and studied its correlation with a series of clinicopathologic features and prognositic parameters of patients with HCC. Results: We found patients with higher Arg-1 expression showed less aggressive features based on less portal vein invasion (χ2 = 10.794, df = 1, p = 0.001), less microvessel invasion (χ2 = 4.247, df = 1, p = 0.039), lower TNM stage (χ2 = 4.992, df = 1, p = 0.025), and better differentiated histology (χ2 = 24.155, df = 1, p < 0.001). Among them, portal vein invasion (p = 0.024, 95% CI 1.010-2.321), microvessel invasion (p = 0.043, 95% CI 1.014-3.225), histology (p = 0.001, 95% CI 2.230-5.934), and TNM stage (p = 0.001, 95% CI 1.364-3.401) have been suggested as prognostic factors in HCC patients. Furthermore, the enhanced expression of Arg-1 in HCC appears to be associated with a lower recurrence rate and prolonged overall survival. Conclusions: These results suggest that Arg-1 may play a tumor suppressive role in HCC and could be a new, promising prognostic biomarker for HCC patients.

Xu C.,Zhejiang University | Jin F.,Zhejiang University | Chen L.,CAS Institute of Genetics and Developmental Biology | Yao X.,Central Hospital of Huzhou
Human Cell | Year: 2010

Currently worldwide attention has focused on the derivation of human embryonic stem cells (hESCs) for future therapeutic medicine. However, the majority of existing hESCs are directly or indirectly exposed to non-human materials during their derivation and/or propagation, which greatly restrict their therapeutic potential. Besides the efforts to improve culture systems, the derivation procedure, especially blastocyst manipulation, needs to be optimized. We adopted a non-contact laser-assisted hatching system in combination with sequential culture process to obtain hatched blastocysts as materials for hESC derivation, and derived a hESC line ZJUhES-1 of a Chinese population without exposure to any non-human materials during blastocyst manipulation. ZJUhES-1 satisfies the criteria of pluripotent hESCs: typically morphological characteristics; the expression of alkaline phosphatase, human telomerase reverse transcriptase and multiple hESC-specific markers including SSEA-3, SSEA-4, TRA-1-60, TRA-1-81, OCT-4, Nanog, Rex-1, Sox-2, UTF-1, Connexins 43 and 45, TERF-1 and TERF-2, Glut-1, BCRP-1/ABCG-2, GDF3, LIN28, FGF4, Thy-1, Cripto1/TDGF1, AC133 as well as SMAD1/2/3/5; extended proliferative capacity; maintenance of a stable male karyotype after long-term cultivation; and robust multiple-lineage developmental potentials both in vivo and in vitro. Moreover, ZJUhES-1 has distinct identity revealed from DNA fingerprinting. Our xeno-free blastocyst manipulation procedure may promote the progression toward clinical-grade hESC derivation. © 2010 The Authors. Human Cell © 2010 Japan Human Cell Society.

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