Chen J.,Third Peoples Hospital |
Chen C.,Central Hospital of Huzhou |
Zhi S.,Third Peoples Hospital
Shanghai Archives of Psychiatry | Year: 2014
Background: Laryngectomy, a common treatment for laryngeal cancer, is a disabling operation that can induce tremendous stress, but little is known about how to alleviate the psychological effects of the operation. Aim: Compare the effectiveness of cognitive behavioral therapy (CBT) and medication in treating anxiety and depression among throat cancer patients after laryngectomy. Methods: Review of medical records of the psychological outpatient clinic in the Third People's Hospital of Huzhou City between March 2009 and May 2013 identified 63 patients with post-laryngectomy depression or anxiety disorders who received 8 weeks of one-on-one treatment with CBT (in which patients responded in writing because they were unable to speak) and 56 patients who received 8 weeks of treatment with buspirone (n=11), sertraline (n=9) or both busipirone and sertraline (n=36). The treatment provided (CBT or medications) was based on the stated preference of the patient. The Zung Self-rating Anxiety Scale (SAS) and the Zung Self-rating Depression Scale (SDS) were administered before and after treatment. Results: After 8 weeks of treatment the mean SAS and SDS scores had decreased significantly in both groups and the prevalence of clinically significant anxiety and depression (based on SAS and SDS cutoff scores) had dropped dramatically. There were, however, no significant differences between the two treatment methods. In the medication group 32% of participants experienced one or more adverse reactions during treatment, but none of these were severe enough to require withdrawal from treatment. Conclusions: CBT is an effective, short-term treatment for reducing the anxiety and depressive symptoms that often occur after an individual is diagnosed with cancer or treated for cancer. There is robust evidence that treatment of these psychological symptoms can improve both the quality of life and course of illness in cancer patients, so oncologists and other clinicians need to regularly screen patients with cancer and other chronic life-threatening conditions for anxiety and depression and, if present, actively promote the treatment of these symptoms. This study shows that CBT can be effective for cancer patients even when they are unable to speak. Copyright© 2014 by Editorial Department of the Shanghai Archives of Psychiatry.
Wu F.,Central Hospital of Huzhou |
Ye R.,Ningbo University |
Xu J.,Traditional Chinese Hospital of Huzhou
Open Medicine (Poland) | Year: 2016
The association between GSTP1 A>G polymorphism and chemosensitivity of osteosarcoma is controversial according to previously published studies. We conducted this meta-analysis to further investigate the role of GSTP1 A>G genetic variation in response to chemotherapy resistance in patients with osteosarcoma. Using the electronic databases of Pubmed, Wanfang and CNIK were searched to find the studies related to the GSTP1 A>G polymorphism and chemosensitivity of osteosarcoma. The genotype of AA, AG and GG were extracted from the chemotherapy sensitivity and chemotherapy resistance group. The association between GSTP1 A>G polymorphism and chemosensitivity was calculated by STATA11.0 software. The correlation between GSTP1 A>G polymorphism and chemotherapy response was assessed by odds ratio (OR) and its 95% confidence interval (95%CI). Four studies with 681 cases were finally included in this meta-analysis. The pooled data indicated that there was no significant association between GSTP1 A>G polymorphism and chemosensitivity in patients with osteosarcoma [Homozygous genetic model (GG vs AA): OR=0.53, 95%CI: 0.25-1.12, P=0.10; recessive genetic model (GG vs GA+AA): OR=0.61, 95%CI:0.34-1.11,P=0.11; and dominant genetic model (GG+AG vs AA): OR=0.67, 95%CI:0.42- 1.07,P=0.10]. No correlation between GSTP1 A>G polymorphism and chemosensitivity was found according to this present meta-analysis. However, the small number of cases in each included study and significant statistical heterogeneity among the trials means the conclusion should be regarded as conservative. © 2016 Wu Fengfeng.
Li J.,Central Hospital of Huzhou |
Wei L.,Soochow University of China |
Sun J.,Soochow University of China |
Guan G.,Central Hospital of Huzhou
Journal of Biomaterials Applications | Year: 2013
In this work, the medium containing ionic products of dicalcium silicates (Ca2SiO4) for culturing MG63 cells was prepared by immersing a titanium alloy plate with the plasma sprayed Ca2SiO4 coatings in DMEM solution. The effect of the ionic products on cellular differentiation, collagen production, and local growth factors (prostaglandin E2 [PGE2] and transforming growth factor-β [TGF-β1]) of osteoblast-like MG63 cells were investigated. The normal DMEM was also used to culture MG63 cells as the control group. Differentiation of cell was evaluated by detecting alkaline phosphatase (ALP) activity and osteocalcin (OC) synthesis as well as their gene expression. Collagen production was analyzed by Sircol assay. The levels of PGE2 and TGF-β1 in culture medium were measured using enzyme-linked immunosorbent assay (ELISA). The gene expressions of TGF-β receptors (TGF-β RI and TGF-β RII) were also measured by real-time PCR technology. MG63 cells cultured in DMEM containing ionic products of Ca2SiO4 coating showed enhanced differentiation and increased collagen production. The results obtained from ELISA showed that the levels of PGE2 and TGF-β1 in experimental group were higher than that in control. The gene expression of TGF-β receptors was upregulated, indicating that more TGF-β1 bonded to their receptors which produce more effects on the osteoblastic activity, leading to enhanced differentiation and synthetic activity of osteoblast. It is concluded that ionic products of Ca2SiO4 coating may enhance cellular differentiation and collagen production by influencing TGF-β1 pathway. © The Author(s) 2011 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.
Lu P.,Central Hospital of Huzhou |
Zhang G.,Zhejiang Cancer Hospital
Chinese Journal of Cancer Prevention and Treatment | Year: 2011
OBJECTIVE: To investigate the optimal methods of fluorescence in situ hybrdization in HER-2 gene detection. METHOD: Totally 55 samples of breast cancer were gathered by bath and direct digestion, dimethylformamide and denaturation law, were compared by detecting the success expression rate of HER-2 gene. RESULTS: In two digest methods, the success rates of hybridization were 86.21% (25/29) and 88.46% (23/26), respectively and with no difference (P>0.05). But hybridization signal ratio were 17.24% (5/29) and 46.15% (12/26), with significant difference (P<0.05). In two digest methods, the success rates were 73.08% (19/26) and 93.10% (27/29), with significant difference (P<0.05). CONCLUSION: Detecting HER-2 gene by fluorescence in situ hybridization, bath and denaturation law hybridization variability have higher success rate.
Wei Y.-H.,Central Hospital of Huzhou |
Xu J.-W.,Central Hospital of Huzhou |
Shen H.-P.,Central Hospital of Huzhou |
Zhang G.-L.,Central Hospital of Huzhou |
And 3 more authors.
World Journal of Gastroenterology | Year: 2014
Splenic artery aneurysm is one of the most common visceral aneurysms, and patients with this type of aneurysm often present without symptoms. However, when rupture occurs, it can be a catastrophic event. Although most of these aneurysms can be treated with percutaneous embolization, some located in uncommon parts of the splenic artery may make this approach impossible. We present a patient with an aneurysm in the proximal splenic artery, close to the celiac trunk, which was treated by laparoscopic ligation only, without resection of the aneurysm, and with long-term preservation of splenic function. © 2014 Baishideng Publishing Group Co., Limited. All rights reserved.
Wang W.X.,Central Hospital of Huzhou
Zhonghua yi xue za zhi | Year: 2010
OBJECTIVE: To observe the clinical results of different concentration NaCl solution in hemorrhagic shock resuscitation. METHODS: The 75 cases with hemorrhagic shock were divided into five groups by means of random number table. Treat the cases with 0.9% (control group), 3%, 4.5%, 6% and 7.5% NaCl solution, respectively. Observe the changes of BP, HR, blood gas, renal function, electrolyte concentration of plasma, before and after treatment. RESULTS: After transfusion, compared with groups of 0.9% NS, the mean BP of patients in other four groups rise more quickly but only the group of 4.5% can last 30 minutes (P < 0.05), and K(+) of plasma in groups of 6%, 7.5% NS decline more obviously (P < 0.05), the group of 4.5% maintain the best lever. CONCLUSION: The mean BP of patients rise quickly and continue for a long time, in the group of 4.5%. Meanwhile the internal environment of the body maintain relatively stable in the group of 4.5%.
Qiu J.,Central Hospital of Huzhou |
Fu Y.-F.,Zhejiang University |
Zhou C.-Y.,Zhejiang University |
Lu W.-G.,Zhejiang University
Chinese Journal of Cancer Prevention and Treatment | Year: 2015
OBJECTIVE: To investigate the expressions of CK2β and p53 in epithelial ovarian cancer tissues and to analysis their correlation with clinical pathological and survival significance. METHODS: 108 patients with epithelial ovarian cancer, 20 patients with benign epithelial ovarian tumor and 20 patients with bordline epithelial ovarian tumor who underwent treatment in Women's Hospital, School of Medicine, Zhejiang University between January 1, 2003 and December 31, 2005 were retrospectively analyzed. Immunohistoehemistry was used to assess the expressions of CK2β and p53. The differences among different epithelial ovarian tumor and the relationships between expression of CK2β, p53 and prognosis in epithelial ovarian cancer were analyzed. RESULTS: The positive expression rate of CK2β and p53 in epithelial ovarian cancer, bordline ovarian tumor and benign ovarian tumor was 63.0%(68/108) and 53.7%(58/108), 30.0%(6/20) and 20.0%(4/20), 0(0/20) and 0(0/20) respectively. The expression intensities of CK2β and p53 in epithelial ovarian cancer were signifieanly higher than that in benign(P<0.001, P<0.001)and bordline epithelial ovarian tumor tissues(P=0.003, P=0.006), and there was significant correlation between CK2β and p53 expression(r=0.377, P<0.001). The expression of CK2β protein in epithelial ovarian cancer was associated with tumor stage(P=0.001), CA125 level (P<0.001) and chemosensitivity(P=0.012); whereas the expression of p53 protein in epithelial ovarian cancer was correlated with histological grade (P=0.027) and CA125 level(P=0.027). Patients with positive expression of CK2β and p53 demonstrated a significantly worse prognosis than that of patients with negative expression of CK2β and p53 for disease-free and overall survival(all P<0.05). Multivariate survival analysis revealed that tumor stage (P<0.001) and positive p53 expression (P=0.018) provided significant independent prognostic value for disease-free survival, whereas tumor stage (P=0.001), CA125 level (P=0.040) and p53 expression (P=0.041) provided significant independent prognostic value for overall survival. CONCLUSIONS: The expressions of CK2β and p53 protein were significantly associated with the development and progression of epithelial ovarian cancer. p53 expression has important predictive value in predicting ovarian cancer survival. ©, 2015, The Editorial Board of Chinese Journal of Cancer Prevention and Treatment. All right reserved.
Zhang M.-J.,Central Hospital of Huzhou |
Yan Q.,Central Hospital of Huzhou |
Zhang G.-L.,Central Hospital of Huzhou |
Wie Y.-H.,Central Hospital of Huzhou |
And 3 more authors.
Clinical Laboratory | Year: 2016
Background: Fragile histidine triad (FHIT), fibronectin (FN), and phosphatase and tensin homology deleted on chromosome ten (PTEN) are widely reported as having abnormal expression in malignant tumors. The role of FHIT, fibronectin, PTEN expression in patients with hepatocellular carcinoma (HCC) has not been characterized. Our study aims to investigate the expression of FHIT, fibronectin, and PTEN in human HCC and their relationship with clinicopathological features and prognosis of HCC. Methods: Immunohistochemistry was used to detect expression of FHIT, FN, and PTEN in tumor tissues from 138 HCC patients. The correlation between their expression and clinicopathological features and prognosis were analyzed. Results: FHIT, fibronectin, and PTEN proteins have different expressions between HCC and adjacent nontumor tissue (χ2 = 5.968, 7.380, 4.962; p < 0.05), which are expressed differently in the groups of different tumor stage, grade, tumor size, tumor number, lymph node metastasis, HBV infection, and cirrhosis in the background of nontumor sections (p < 0.05). In the FHIT and fibronectin positive expression group, the cumulative survival times were shorter than those in the negative expression groups (χ2 = 4.443, 9.867; p < 0.05), and in the PTEN positive expression group the cumulative survival times were longer than in the negative expression group (χ2 = 4.199; p < 0.05). Conclusions: FHIT, fibronectin, and PTEN were abnormally expressed in HCC cells, which have stimulative or suppressive effects on HCC carcinogenesis and progression. FHIT and fibronectin can be used as negative makers for prognosis and PTEN as a positive one. © 2016, Verlag Klinisches Labor GmbH. All rights reserved.
PubMed | Central Hospital of Huzhou
Type: Journal Article | Journal: Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology | Year: 2010
To investigate the relationship between the hepatitis B virus (HBV) infection in pregnant women and intrauterine infection in local region.The markers of hepatitis B (HBVM) were determined by time-resolved fluoroimmunoassay and HBV-DNA were determined by FQ-PCR.A total of 1262 pregnant women were examined the HBVM, 2.6%, 38.2%, 0.9%, 22.6%, 23.1% subjects were identified HBsAg, HBsAb, HBeAg, HBeAb, HBcAb positive respectively. In 33 cases of serum HBsAg-positive pregnant women, HBV-DNA were observed in most of 11 cases of pregnant women with HBeAg-positive and intrauterine infection rates were 6/11. In contrast, 22 cases of pregnant women with HBeAg negative, HBV-DNA were detected lowly-loaded and intrauterine infection rates were 2/22 (P < 0.01). Intrauterine infection rates of HBV in pregnant women with HBsAg-positive were 24.2% (8/33).HBV infective rates in pregnant women in the local region were low. Pregnant women with serum HBeAg positive and HBV-DNA high-loaded were prone to intrauterine infection.
PubMed | Central Hospital of Huzhou
Type: Journal Article | Journal: Zhonghua yi xue za zhi | Year: 2010
To observe the clinical results of different concentration NaCl solution in hemorrhagic shock resuscitation.The 75 cases with hemorrhagic shock were divided into five groups by means of random number table. Treat the cases with 0.9% (control group), 3%, 4.5%, 6% and 7.5% NaCl solution, respectively. Observe the changes of BP, HR, blood gas, renal function, electrolyte concentration of plasma, before and after treatment.After transfusion, compared with groups of 0.9% NS, the mean BP of patients in other four groups rise more quickly but only the group of 4.5% can last 30 minutes (P < 0.05), and K(+) of plasma in groups of 6%, 7.5% NS decline more obviously (P < 0.05), the group of 4.5% maintain the best lever.The mean BP of patients rise quickly and continue for a long time, in the group of 4.5%. Meanwhile the internal environment of the body maintain relatively stable in the group of 4.5%.