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Zhang R.,Shanghai Key Laboratory of Stomatology | Zhang R.,Shanghai JiaoTong University | Shi H.M.,Shanghai JiaoTong University | Li Q.H.,Stomatological Hospital | And 12 more authors.
Journal of Craniofacial Surgery | Year: 2013

Three-dimensional measurement of the pharyngeal airway has been widely used, but the three-dimensional reconstruction of pharyngeal airway has been performed in various ways, especially during the anterior boundary demarcation of the nasopharyngeal airway and oropharyngeal airway. This would inevitably affect the measurement and lead to noncomparison between different studies. Our study provided a novel method for anterior boundary demarcation of pharyngeal airway that defined the anterior boundary of nasopharyngeal airway as the choana according to the anatomical definition and defined the anterior boundary of oropharyngeal airway as a plane perpendicular to the long axis of soft palate and through the intersections of the lateral space and inferior space to soft palate according to the physiologic characteristics of soft palate. By 2-step segmentation, a three-dimensional image of pharyngeal airway was eventually reconstructed.Ten computed tomographic scans of pharyngeal airway were included for the anterior boundary demarcation and three-dimensional reconstruction by a medical imaging software (Surgicase 5.0; Materialise Interactive Medical Image Control System, Leuven, Belgium), with the volume and surface area being calculated. By using intraclass correlation coefficient, the reliability between intra-and interobservers of this method was well tested.The method established in this study for anterior boundary demarcation and three-dimensional reconstruction of pharyngeal airway is highly reliable and could more veritably reflect the intrinsic anatomical characteristics of the pharyngeal airway. © 2013 by Mutaz B. Habal, MD.


Zhang Y.,Central Hospital of Huangpu District | Wang Z.,Shanghai University
Tumor | Year: 2011

Objective: To investigate the effect of expression of metastasis-associated in colon cancer 1 (MACC1) on the liver metastasis from colon cancer. Methods: The SW1116 and HCT116 cells, which had different liver metastasis abilities, were infected with lentiviral vector pCDH cDNA/ green fluorescent protein (GFP) used as a tracer. The liver metastasis model in BALB/c-nu/nu nude mice was established by spleen injection of these two infected colon cancer cell lines. The liver metastasis from colon cancer cells was observed under a fluorescence dissecting microscope, and the liver metastasis rate was calculated. The expression levels of MACC1 protein in SW1116 and HCT116 cells were detected by Western blotting, and the correlation between the MACC1 protein expression and the liver metastasis rate was evaluated. Results: The liver metastasis rate of SW1116 cells was higher than that of HCT116 cells. The MACC1 protein expression in SW1116 cells was high, but it was low in HCT116 cells, and the difference between the two colon cancer cells was significant (P<0.01). Conclusion: The expression level of MACC1 may have a positive association with the liver metastatic ability of colon cancer cells.


Wang C.,Central Hospital of Huangpu District | Pan Y.-H.,Central Hospital of Huangpu District | Shan M.,Central Hospital of Huangpu District | Xu M.,Central Hospital of Huangpu District | And 2 more authors.
International Journal of Molecular Sciences | Year: 2015

Breast cancer is one of the most common and lethal cancers in women. As a hub gene involved in a diversity of tumors, the ubiquitin-conjugating enzyme H10 (UbcH10), may also play some roles in the genesis and development of breast cancer. In the current study, we found that the expression of UbcH10 was up-regulated in some breast cancer tissues and five cell lines. We established a dual drug resistant cell line MCF-7/EPB (epirubicin)/TXT (docetaxel) and a lentiviral system expressing UbcH10 shRNA to investigate the effects of UbcH10 knockdown on the chemosensitivity of MCF-7/EPB/TXT cells to epirubicin and docetaxel. The knockdown of UbcH10 inhibited the proliferation of both MCF-7 and MCF-7/EPB/TXT cells, due to the G1 phase arrest in cell cycle. Furthermore, UbcH10 knockdown increased the sensitivity of MCF-7/EPB/TXT cells to epirubicin and docetaxel and promoted the apoptosis induced by these two drugs. Protein detection showed that, in addition to inhibiting the expression of Ki67 and cyclin D1, UbcH10 RNAi also impaired the increased BCL-2 and MDR-1 expression levels in MCF-7/EPB/TXT cells, which may contribute to abating the drug resistance in the breast cancer cells. Our research in the current study demonstrated that up-regulation of UbcH10 was involved in breast cancer and its knockdown can inhibit the growth of cancer cells and increase the chemosensitivity of the dual drug resistant breast cancer cells to epirubicin and docetaxel, suggesting that UbcH10 may be a promising target for the therapy of breast cancer. © 2015 by the authors; licensee MDPI, Basel, Switzerland.


Xu M.,Central Hospital of Huangpu District | Ye M.-N.,Central Hospital of Huangpu District | Wang C.,Central Hospital of Huangpu District | Ye H.,Central Hospital of Huangpu District
Journal of Shanghai Jiaotong University (Medical Science) | Year: 2015

Objective: To explore the clinicopathological features of micropapillary pure mucinous carcinoma (MPPMC) and MPPMC combined with invasive micropapillary carcinoma (IMPC). Methods: Clinical pathological data of 8 cases of MPPMC and 5 cases of MPPMC combined with IMPC were collected and the histological morphology, immune phenotypes, and clinical features were analyzed. Results: Transitional regions were observed in 5 cases of MPPMC combined with IMPC. The mucus around the micropapillary gradually disappeared and formed IMPC. The expression of Ki-67 in cancer tissues significantly increased after the transformation of MPPMC to IMPC. The rate of lymph node metastasis of the MPPMC group was only 12.5%, while that of the MPPMC combined with IMPC group was 80%. The main type of cancer in lymph node metastases was IMPC. Conclusion: Breast MPPMC and IMPC may be in the same tumor lineages. The biological behavior of MPPMC combined with IMPC is more serious. The treatment of MPPMC combined with IMPC should reference that of IMPC. ©, 2015, Editorial Department of Journal of Shanghai Second Medical University. All right reserved.


Shi Y.,Central Hospital of Huangpu District | Qiu G.-X.,Central Hospital of Huangpu District | Zhou Z.-C.,Central Hospital of Huangpu District | Cui S.-S.,Central Hospital of Huangpu District
Chinese Journal of New Drugs | Year: 2012

Objective: To evaluate the therapeutical effect of compound matrine injection on chemotherapeutic drug-induced liver injury. Methods: A total of 240 patients clinically diagnosed as drug-induced liver injury in oncology department were randomized to receive either a compound matrine injection or a placebo for 2 weeks. Then, the hepatic functions between the compound matrine injection and placebo groups were compared. Results: After two weeks of treatment, there were 65 patients' liver function recoveried in experiment group, which accounted for 50.78%. There were 42 patients' liver function recoveried in control group, which accounted for 37.5%. According to the subtypes, the effective rate on hepatocellular type in compound matrine injection group was significantly superior to that in placebo group (P<0.05), but the effective rate on cholestatic type and mixed type was not significantly different from that in placebo group. Conclusion: Compound matrine injection has a hepatic protective effect on chemotherapeutic drug-induced liver injury.

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