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Wang C.,Tongji University | Wang C.,Central Hospital of Huangpu District | Shan M.,Central Hospital of Huangpu District | Niu R.-J.,Central Hospital of Huangpu District | And 3 more authors.
Journal of Shanghai Jiaotong University (Medical Science) | Year: 2017

Objective: To investigate the expression of CD147 and its clinical significance in triple-negative breast cancer (TNBC) tissues and analyze the association between CD147 expression and the prognosis. Methods: Clinicopathological and follow-up data of 110 patients with TNBC were collected. The immunohistochemical assay was performed for CD147 and the association of CD147 expression with clinicopathological characteristics and the prognosis was analyzed. Results: The positive expression rate of CD147 in TNBC tissues was 81.82% (90/110). CD147 expression was positively correlated to histological grade, high expression of Ki-67, and positive expression of p53 (P=0.000, 0.047, and 0.046, respectively) and was not significantly correlated to age, tumor size, and axillary lymph node metastasis (P>0.05). Survival analysis showed that CD147 expression intensity was associated with overall survival and disease-free survival (P=0.024 and 0.001, respectively). Multivariate regression analysis indicated that CD147 and Ki-67 were risk factors for the prognosis of TNBC. The higher the positive expression rates were, the shorter the survival was. Conclusion: CD147 is highly expressed in TNBC tissues and is closely associated with the prognosis. It can be used as an independent factor for predicting the prognosis of TNBC. Clinical application of CD147 antibody may be an important target therapy for TNBC in the future. © 2017, Editorial Department of Journal of Shanghai Second Medical University. All right reserved.


Wang C.,Central Hospital of Huangpu District | Pan Y.-H.,Central Hospital of Huangpu District | Shan M.,Central Hospital of Huangpu District | Xu M.,Central Hospital of Huangpu District | And 2 more authors.
International Journal of Molecular Sciences | Year: 2015

Breast cancer is one of the most common and lethal cancers in women. As a hub gene involved in a diversity of tumors, the ubiquitin-conjugating enzyme H10 (UbcH10), may also play some roles in the genesis and development of breast cancer. In the current study, we found that the expression of UbcH10 was up-regulated in some breast cancer tissues and five cell lines. We established a dual drug resistant cell line MCF-7/EPB (epirubicin)/TXT (docetaxel) and a lentiviral system expressing UbcH10 shRNA to investigate the effects of UbcH10 knockdown on the chemosensitivity of MCF-7/EPB/TXT cells to epirubicin and docetaxel. The knockdown of UbcH10 inhibited the proliferation of both MCF-7 and MCF-7/EPB/TXT cells, due to the G1 phase arrest in cell cycle. Furthermore, UbcH10 knockdown increased the sensitivity of MCF-7/EPB/TXT cells to epirubicin and docetaxel and promoted the apoptosis induced by these two drugs. Protein detection showed that, in addition to inhibiting the expression of Ki67 and cyclin D1, UbcH10 RNAi also impaired the increased BCL-2 and MDR-1 expression levels in MCF-7/EPB/TXT cells, which may contribute to abating the drug resistance in the breast cancer cells. Our research in the current study demonstrated that up-regulation of UbcH10 was involved in breast cancer and its knockdown can inhibit the growth of cancer cells and increase the chemosensitivity of the dual drug resistant breast cancer cells to epirubicin and docetaxel, suggesting that UbcH10 may be a promising target for the therapy of breast cancer. © 2015 by the authors; licensee MDPI, Basel, Switzerland.


Zhang Y.,Central Hospital of Huangpu District | Wang Z.,Shanghai University
Tumor | Year: 2011

Objective: To investigate the effect of expression of metastasis-associated in colon cancer 1 (MACC1) on the liver metastasis from colon cancer. Methods: The SW1116 and HCT116 cells, which had different liver metastasis abilities, were infected with lentiviral vector pCDH cDNA/ green fluorescent protein (GFP) used as a tracer. The liver metastasis model in BALB/c-nu/nu nude mice was established by spleen injection of these two infected colon cancer cell lines. The liver metastasis from colon cancer cells was observed under a fluorescence dissecting microscope, and the liver metastasis rate was calculated. The expression levels of MACC1 protein in SW1116 and HCT116 cells were detected by Western blotting, and the correlation between the MACC1 protein expression and the liver metastasis rate was evaluated. Results: The liver metastasis rate of SW1116 cells was higher than that of HCT116 cells. The MACC1 protein expression in SW1116 cells was high, but it was low in HCT116 cells, and the difference between the two colon cancer cells was significant (P<0.01). Conclusion: The expression level of MACC1 may have a positive association with the liver metastatic ability of colon cancer cells.


PubMed | Central Hospital of Huangpu District and Shanghai University
Type: Journal Article | Journal: International journal of molecular sciences | Year: 2015

Breast cancer is one of the most common and lethal cancers in women. As a hub gene involved in a diversity of tumors, the ubiquitin-conjugating enzyme H10 (UbcH10), may also play some roles in the genesis and development of breast cancer. In the current study, we found that the expression of UbcH10 was up-regulated in some breast cancer tissues and five cell lines. We established a dual drug resistant cell line MCF-7/EPB (epirubicin)/TXT (docetaxel) and a lentiviral system expressing UbcH10 shRNA to investigate the effects of UbcH10 knockdown on the chemosensitivity of MCF-7/EPB/TXT cells to epirubicin and docetaxel. The knockdown of UbcH10 inhibited the proliferation of both MCF-7 and MCF-7/EPB/TXT cells, due to the G1 phase arrest in cell cycle. Furthermore, UbcH10 knockdown increased the sensitivity of MCF-7/EPB/TXT cells to epirubicin and docetaxel and promoted the apoptosis induced by these two drugs. Protein detection showed that, in addition to inhibiting the expression of Ki67 and cyclin D1, UbcH10 RNAi also impaired the increased BCL-2 and MDR-1 expression levels in MCF-7/EPB/TXT cells, which may contribute to abating the drug resistance in the breast cancer cells. Our research in the current study demonstrated that up-regulation of UbcH10 was involved in breast cancer and its knockdown can inhibit the growth of cancer cells and increase the chemosensitivity of the dual drug resistant breast cancer cells to epirubicin and docetaxel, suggesting that UbcH10 may be a promising target for the therapy of breast cancer.


Wang C.,Central Hospital of Huangpu District | Shan M.,Central Hospital of Huangpu District | Pan Y.-H.,Central Hospital of Huangpu District | Xu M.,Central Hospital of Huangpu District | Bao J.-L.,Central Hospital of Huangpu District
Journal of Shanghai Jiaotong University (Medical Science) | Year: 2014

Objective: To investigate the effects of UbcH10 gene silencing on the drug resistance of human breast cancer cell MCF-7/TXT. Methods: The shRNA expression vectors were constructed using the siRNA sequences which were designed based on the coding sequence of UbcH10. MCF-7/TXT cells were then infected by lentivirus. After being infected for 72 h, the infection efficiency was observed through the fluorescent marker and infected cells were collected. The variations of UbcH10 mRNA and protein levels of infected cells were detected by the Real-Time PCR and Western blotting, respectively. The effects of gene silencing on the sensitivity of chemotherapeutic drugs for MCF-7/TXT cells were detected by the CCK-8. Results: The gene silencing test of MCF-7/TXT cells was successfully. After being infected for 72 h, the infection efficiency was about 90% and UbcH10 mRNA and protein levels of infected cells significantly decreased. Compared to the control group, the differences were statistically significant (P<0.05). Silencing the UbcH10 gene significantly enhanced the drug sensitivity of taxotere towards MCF-7/TXT cells. IC50 values of taxotere of the gene silencing group at different time points were significantly lower than those of the control group. The differences were statistically significant (P<0.05). Conclusion: Silencing the UbcH10 gene can significantly enhance the chemotherapy sensitivity of drug resistant breast cancer cell MCF-7/TXT towards taxotere. ©, 2014, Editorial Department of Journal of Shanghai Second Medical University. All right reserved.


Xu M.,Central Hospital of Huangpu District | Ye M.-N.,Central Hospital of Huangpu District | Wang C.,Central Hospital of Huangpu District | Ye H.,Central Hospital of Huangpu District
Journal of Shanghai Jiaotong University (Medical Science) | Year: 2015

Objective: To explore the clinicopathological features of micropapillary pure mucinous carcinoma (MPPMC) and MPPMC combined with invasive micropapillary carcinoma (IMPC). Methods: Clinical pathological data of 8 cases of MPPMC and 5 cases of MPPMC combined with IMPC were collected and the histological morphology, immune phenotypes, and clinical features were analyzed. Results: Transitional regions were observed in 5 cases of MPPMC combined with IMPC. The mucus around the micropapillary gradually disappeared and formed IMPC. The expression of Ki-67 in cancer tissues significantly increased after the transformation of MPPMC to IMPC. The rate of lymph node metastasis of the MPPMC group was only 12.5%, while that of the MPPMC combined with IMPC group was 80%. The main type of cancer in lymph node metastases was IMPC. Conclusion: Breast MPPMC and IMPC may be in the same tumor lineages. The biological behavior of MPPMC combined with IMPC is more serious. The treatment of MPPMC combined with IMPC should reference that of IMPC. ©, 2015, Editorial Department of Journal of Shanghai Second Medical University. All right reserved.


Shan M.,Central Hospital of Huangpu District | Wang C.,Central Hospital of Huangpu District | Yu Y.-M.,Central Hospital of Huangpu District | Lu Y.-W.,Central Hospital of Huangpu District | And 2 more authors.
Journal of Shanghai Jiaotong University (Medical Science) | Year: 2015

Objective: To evaluate the clinical value of breast fiberoptic ductoscopy for the diagnosis of nipple discharge. Methods: Diagnoses of 3 427 cases of nipple discharge by fiberoptic ductoscopy and pathological diagnoses of 1 789 cases of them undergoing surgical treatments were retrospectively analyzed. Results: Among 3 427 cases of nipple discharge examined by fiberoptic ductoscopy, there were 2 051 cases of intraductal neoplasm (benign or malignant). A total of 1 532 patients with benign neoplasm undergoing surgery and 1 176 of them were pathologically diagnosed with central or peripheral intraductal papilloma. There were 80 patients with malignant neoplasm undergoing surgery and 52 of them were pathologically diagnosed with breast cancer. The coincidence rate of diagnosis of central or peripheral intraductal papilloma and breast cancer by fiberoptic ductoscopy was 76.76% and 83.87%, respectively. Conclusion: Diagnosis of nipple discharge by fiberoptic ductoscopy has a certain accuracy and is a good examination method for patients with nipple discharge. ©, 2015, Editorial Department of Journal of Shanghai Second Medical University. All right reserved.


Wang C.,Central Hospital of Huangpu District | Yu Y.-M.,Central Hospital of Huangpu District | Lu Y.-W.,Central Hospital of Huangpu District | Shan M.,Central Hospital of Huangpu District | And 2 more authors.
Academic Journal of Second Military Medical University | Year: 2014

Objective To observe the effect of the UbcH10 gene silencing on inhibition effect of doxorubicin against in vivo tumor formation of drug-resistant breast cancer MCF-7/ADR cells. Methods An MCF-7/ADR-UbcH10-RNAi cell line with UbcH10 gene silenced was established. Then MCF-7/ADR-UbcH10-RNAi cells and the control cells at logarithmic phase were inoculated into nude mice to establish the subcutaneous tumor model. Doxorubicin or normal saline was administered for a consecutive of two weeks, and then one week later the tumor volumes were determined to analyze the effects of UbcH10 gene silencing on inhibition of tumor formation by doxorubicin. Meanwhile, Western blotting analysis was used to examine the protein expression of UbcH10 and BCL-2; the relationship between UbcH10 and chemosensitivity of tumor cells was analyzed. Results The MCF-7/ADR-UbcH10-RNAi cell line with UbcH10 gene silenced was successfully established with the lentiviral experimental system. The nude mice tumor models were established three weeks after subcutaneous inoculation of tumor cells. The tumor inhibitory rate was 4.16% in the doxorubicin group, which was not significantly different from the control group (P>0.05); while the tumor inhibitory rate was 41.8% in UbcH10-RNAi+doxorubicin group, which was significantly higher than that in the control group (P<0.05). The results of Western blotting analysis showed that the expression levels of UbcH10 in MCF-7/ADR group, MCF-7/ADR+doxorubicin group, and MCF-7/ADR-UbcH10-RNAi+doxorubicin group were 0.81±0.16, 0.78±0.12, and 0.18±0.04, respectively, with the latter being significantly lower than the former two (P<0.05); BCL-2 protein levels in tumors were consistent with those of UbcH10. Conclusion UbcH10 gene silencing can markedly enhance the in vivo sensitivity of drug resistant breast cancer cells to Doxorubicin. © 2015, Second Military Medical University Press. All rights reserved.


Shi Y.,Central Hospital of Huangpu District | Qiu G.-X.,Central Hospital of Huangpu District | Zhou Z.-C.,Central Hospital of Huangpu District | Cui S.-S.,Central Hospital of Huangpu District
Chinese Journal of New Drugs | Year: 2012

Objective: To evaluate the therapeutical effect of compound matrine injection on chemotherapeutic drug-induced liver injury. Methods: A total of 240 patients clinically diagnosed as drug-induced liver injury in oncology department were randomized to receive either a compound matrine injection or a placebo for 2 weeks. Then, the hepatic functions between the compound matrine injection and placebo groups were compared. Results: After two weeks of treatment, there were 65 patients' liver function recoveried in experiment group, which accounted for 50.78%. There were 42 patients' liver function recoveried in control group, which accounted for 37.5%. According to the subtypes, the effective rate on hepatocellular type in compound matrine injection group was significantly superior to that in placebo group (P<0.05), but the effective rate on cholestatic type and mixed type was not significantly different from that in placebo group. Conclusion: Compound matrine injection has a hepatic protective effect on chemotherapeutic drug-induced liver injury.


Wang C.,Central Hospital of Huangpu District | Hong Y.,Central Hospital of Huangpu District | Yu Y.-M.,Central Hospital of Huangpu District | Shan M.,Central Hospital of Huangpu District | And 2 more authors.
Journal of Shanghai Jiaotong University (Medical Science) | Year: 2016

Objective: To investigate the correlation between metastasis-associated protein 3 (MTA3) and the prognosis of patients with breast cancer. Methods: Tissue microarrays were made using 160 breast cancer samples and contained epidermal growth factor receptor (EGFR) data, estrogen receptor (ER) data, and follow-up information. The expression of MTA3 protein was detected by immunohistochemical method and the correlation between the expression of MTA3 and the prognosis was analyzed. Results: For EGFR+/ER- breast cancer, the overall survival of patients with high expression of MTA3 in cancer tissues was longer (67.9% and 41.2%, P=0.059). For EGFR-/ER+ breast cancer, the overall survival of patients with low expression of MTA3 in cancer tissues was longer (100.0% and 72.0%, P=0.039). For EGFR+/ER+ breast cancer, the expression of MTA3 in cancer tissues did not correlate to the prognosis (P=0.405). Conclusion: The function of MTA3 may be bi-directionally regulated by EGFR and ER pathways, so as to affect the prognosis of patients with breast cancer. © 2016, Editorial Department of Journal of Shanghai Second Medical University. All right reserved.

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