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Guo S.-J.,Central Hospital of Handan City | Wang C.-M.,Hebei University of Engineering
Journal of Dalian Medical University | Year: 2015

Objective: To study the effects of R (+) pramipexole (R + PPX) on the generation of reactive oxygen species (ROS) and to investigate the protective effects of R + PPX on brain tissue after cerebral ischemia in rats and provide experimental basis for effective treatment for ischemic stroke. Methods: The rat model of cerebral ischemia for 2 h and reperfusion was established by thread occlusion. Ninty rats were divided into three groups randomly: sham group,middle cerebral artery occlusion (MCAO) group and the R + PPX + MCAO group. After reperfusion for 8 h and 24 h, the volume of cerebral infarct was determined by the TTC and LOZEX - F image scanning, and the ROS positive cell number was monitored with the new fluorescent probe H2DCF - DA. Results: No cerebral infarction was observed in the sham group but the cerebral infarct volume increased significantly after 8 h and 24 h reperfusion in both MCAO group and R + PPX + MCAO group (P <0.05). After reperfusion for 8 h, the cerebral infarct volume in R + PPX + MCAO group was higher and the number of H2DCFA - DA positive cells was lower than that in MCAO group respectively (P < 0.05), while both of them were not statistical different between the two groups after reperfusion for 24 h (P > 0.05). Conclusion: R + PPX intervention might reduce the cerebral infarct volume and protect the brain by decreasing the generation of ROS cells in early stage of cerebral ischemia in rats.

Yao G.-D.,Central Hospital of Handan City | Huo H.-Q.,Central Hospital of Handan City | Li P.,Central Hospital of Handan City | Liu A.-M.,Central Hospital of Handan City
Chinese Journal of Cancer Prevention and Treatment | Year: 2011

OBJECTIVE: To explore the anti-tumor function differences of original generation CIK cells between normal and stomach neoplasms patients, and detect CIK cells anti-tumor effect. METHODS: Individual nucleus cells of normal people and gastric cancer patients were separated, and CD3McAb, IL-1α, IFN-γ, IL-2 were added respectively. MTT method was used to detect kill activity of two CIK cells. RESULTS: On the first day, CD3 +/CD56 + was expressed in (1.064±0.22)% normal people and (1.031±0.13)% gastric cancer patients. After the cell factors stimulation, the expression rates were (28.66±2.0)%, and (17.63±2.22)% on the 7th day, and (57.14±1.96)% and (48.53±2.19)% on the 14th day accounted for (P<0.05). Its kill tumor ability also was higher than that of the patient's own killer ability (P<0.01). CONCLUSION: Both the normal people and gastric cancer patients CIK cells have anti-tumor effect, and the normal people's CIK cell proliferation capacity and anti-tumor activity are higher than those of the tumor patients.

Wang L.,Central Hospital of Handan City | Zhang H.-F.,Central Hospital of Handan City | Wang H.,Central Hospital of Handan City | Li X.-Q.,Central Hospital of Handan City
Chinese Journal of New Drugs | Year: 2011

A female patient with palms and joint pain for ten months received aceclofenac tablets, 0.1 g, bid, q12h. Six days later, her platelets sharply dropped to 1.0×10 9·L -1. We analyzed and stated here the pharmacological mechanisms of adverse reactions and the dealing measures for it. It is expected to pay attention to the potential adverse reactions of aceclofenac tablets.

Wang G.-Y.,Peking University | Zhang S.-L.,Peking University | Wang X.-R.,Peking University | Feng M.,Peking University | And 65 more authors.
Clinical Rheumatology | Year: 2015

The aim of this study is to investigate the remission rate of rheumatoid arthritis (RA) in China and identify its potential determinants. A multi-center cross-sectional study was conducted from July 2009 to January 2012. Data were collected by face-to-face interviews of the rheumatology outpatients in 28 tertiary hospitals in China. The remission rates were calculated in 486 RA patients according to different definitions of remission: the Disease Activity Score in 28 joints (DAS28), the Simplified Disease Activity Index (SDAI), the Clinical Disease Activity Index (CDAI), and the American College of Rheumatology/European League Against Rheumatism (ACR/EULAR) Boolean definition. Potential determinants of RA remission were assessed by univariate and multivariate analyses. The remission rates of RA from this multi-center cohort were 8.6 % (DAS28), 8.4 % (SDAI), 8.2 % (CDAI), and 6.8 % (Boolean), respectively. Favorable factors associated with remission were: low Health Assessment Questionnaire (HAQ) score, absence of rheumatoid factor (RF) and anti-cyclic citrullinated peptide (anti-CCP), and treatment of methotrexate (MTX) and hydroxychloroquine (HCQ). Younger age was also predictive for the DAS28 and the Boolean remission. Multivariate analyses revealed a low HAQ score, the absence of anti-CCP, and the treatment with HCQ as independent determinants of remission. The clinical remission rate of RA patients was low in China. A low HAQ score, the absence of anti-CCP, and HCQ were significant independent determinants for RA remission. © 2014, International League of Associations for Rheumatology (ILAR).

Yao G.,Central Hospital of Handan City | Liu C.,China Three Gorges University | Huo H.,Central Hospital of Handan City | Liu A.,Central Hospital of Handan City | And 5 more authors.
Oncology Letters | Year: 2013

Chaenomeles speciosa Nakai is commonly used in traditional Chinese medicine for a variety of health-promoting effects. The present study aimed to investigate the antitumor effects of Chaenomeles speciosa Nakai. The tumor-inhibitory activity of the ethanol extract of Chaenomeles speciosa Nakai (EEC) was evaluated by in vitro growth assays of tumor cells and in vivo H22 tumor formation assays in mice. Mitochondrial membrane potential and DNA ladder assays were used to detect tumor cell apoptosis in the presence of EEC. To investigate the cellular targets of EEC, the immunomodulatory genes PD-L1, Foxp3 and TGF-β were detected in the tumor tissue using reverse transcription polymerase chain reaction (RT-PCR). Immune responses were determined by hemolysis and lymphocyte proliferation assays. EEC markedly inhibited the proliferation of the H22 cells in a dose-dependent manner. Moreover, it induced DNA fragmentation and decreased the mitochondrial membrane potential. In vivo, EEC inhibited tumor growth and enhanced the immune responses in mice, while the expression of PD-L1, Foxp3 and TGF-β was inhibited in the tumor tissue. These results provide the first evidence that EEC may inhibit tumor growth by directly killing tumor cells and enhancing immune function. Thus, it is a natural source for safe anticancer medicine.

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