Zhao H.,Central Hospital of Fengxian District |
Xiao H.-J.,Central Hospital of Fengxian District
Chinese Journal of Tissue Engineering Research | Year: 2013
Background: Cannulated screw tension band combined with wire fixation is most commonly used for treatment of patella fractures. Objective: To evaluate the clinical outcomes of combination of cannulated screw tension band and wire fixation in the treatment of patella fractures. Methods: A literature search method was employed to retrieve relevant articles addressing cannulated screw tension band and wire fixation of patella fractures. In-depth analysis of literatures meeting the study criteria was carried out. Patients with patella fractures who received cannulated screw tension band and wire fixation were followed up to evaluate the postoperative fracture healing, complications and functional recovery of the knee joint at the injury side. Then, the clinical efficacy of cannulated screw tension band combined with wire fixation was analyzed. Results and Conclusion: After treatment with cannulated screw tension band and wire fixation, patients with patella fractures exhibited good fracture healing and low probability of complications, including screw or wire loosening, broken, and shedding. Functional recovery of the affected knee was up to 90%. Clinical case analysis showed that cannulated screw tension band combined with wire fixation achieved good outcomes in the treatment of patella fracture, with over 90% functional recovery of the affected knee, which is consistent with the reported results. It indicates that the cannulated screw tension band combined with wire fixation is one of the ideal and reliable methods in use for internal fixation of patella fractures.
Sun W.,Central Hospital of Fengxian District
Chinese Traditional and Herbal Drugs | Year: 2012
Objective To study the chemical constituents of Phyllanthus amarus. Methods Chromatography on silica gel column, Sephadex LH-20 columm, recrystallization, and preparative HPLC technique were used to isolate and purify the compounds. Spectroscopic methods including UV, IR, EI-MS, 1H-NMR, 13C-NMR, HMQC, and HMBC were used to elucidate the structures of compounds. Results Nine compounds were obtained and identified as palmitic acid (1), 4-hydrobenzal dehyde (2), oleanolic acid (3), stigmast-5-en-3-ol, oleate (4), 4-hydroxy-3-methoxy-benzoic acid (5), daucosterol (6), apigenin (7), luteolin (8), and phyllanoside (9). Conclusion Compound 9 is a new compound, named phyllanoside. Compounds 4-8 are isolated from the plant for the first time.
Li M.,Central Hospital of Fengxian District |
Wu M.Y.,Central Hospital of Fengxian District |
Han Y.,Central Hospital of Fengxian District |
Li R.,Central Hospital of Fengxian District
European Journal of Gynaecological Oncology | Year: 2012
We present a case of a 56-year-old woman with primary infertility who complained of amenorrhea for five months and vaginal bleeding for two months. Initially she was misdiagnosed with endometrial cancer due to her disease history and older age, but eventually a diagnosis of complete hydatidiform mole was confirmed and then laparoscopic total hysterectomy was performed. The patient has been followed-up as an outpatient for more than one year, and hads an excellent prognosis. To our knowledge, this is the first case of hydatidiform mole in a woman with primary infertility during the perimenopausal stage. Even though hydatidiform mole is rare in primary infertility patients during perimenopause, it should always be considered in case of misdiagnosis.
Zhu J.,Shanghai JiaoTong University |
Zhao Y.,Central Hospital of Fengxian District |
Liu F.,Central Hospital of Fengxian District |
Huang Y.,Shanghai JiaoTong University |
And 2 more authors.
BMC Musculoskeletal Disorders | Year: 2012
Background: To define the sites where the lateral femoral cutaneous nerve (LFCN) is more easily visualized and to describe the anatomical variations of the LFCN. Methods. A total of 240 LFCNs in 120 volunteers were evaluated with 18 MHz ultrasound; the intermuscular space between the tensor fasciae latae muscle and the sartorius was used as an initial sonographic landmark. The time taken to identify the nerve was recorded. The number of nerve branches at the level of the inguinal ligament (IL) and the relationship between the LFCN and the IL was assessed. The nerve cross-sectional area (CSA) of the LFCN and the distance between the LFCN and the anterior superior iliac spine was measured. Results: Each nerve was identified using ultrasound in all participants. The mean time for identifying the nerve was 7s for unilateral LFCNs. The nerve passed under the IL in 198 cases, whereas in 44 cases, it passed through to the IL. The LFCN consisted of 1-4 branches just after its passage under or through the IL. The CSA of the LFCN was 1.04±0.44 mm§ssup§2§esup§, and the mean distance between the LFCN and the anterior superior iliac spine was 15.6 ± 4.2 mm. Conclusions: It is easier to identify the LFCN if the intermuscular space between the tensor fasciae latae muscle and the sartorius is used as an initial sonographic landmark. The anatomical variation of the LFCN can be viewed with high-frequency ultrasound. © 2012 Zhu et al.; licensee BioMed Central Ltd.
Zhi Y.,Central Hospital of Fengxian District |
Shoujun H.,Soochow University of China |
Yuanzhou S.,Central Hospital of Fengxian District |
Haijun L.,Central Hospital of Fengxian District |
And 4 more authors.
FEBS Letters | Year: 2012
Interleukin-6 (IL-6) induced STAT3 activation is viewed as crucial for multiple tumor growth and metastasis, including colon cancer. However, the molecular mechanisms remain largely unexplored. Here, we show that expression of ubiquitin-specific protease 7 (USP7), a deubiquitylating enzyme, is decreased in STAT3-positive tumors. IL-6 administration or transfection of a constitutively activated STAT3 in SW480 cells also repressed USP mRNA expression. Using luciferase reporter and ChIP assay, we found that STAT3 bound to the promoter region of USP7 and inhibited its activity through recruiting HDAC1. As a result of the decline of USP7 expression, endogenous P53 protein level was decreased. Thus, our results suggest a previously unknown STAT3-USP7-P53 molecular network controlling colon cancer development. Structured summary of protein interactions:: STAT3 physically interacts with HDAC1 by anti bait coimmunoprecipitation (View interaction). © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Liu X.-J.,Shanghai JiaoTong University |
Kong F.-Z.,Shanghai JiaoTong University |
Kong F.-Z.,Central Hospital of Fengxian District |
Wang Y.-H.,Shanghai JiaoTong University |
And 10 more authors.
PLoS ONE | Year: 2013
Lumican is a dermatan sulfate proteoglycan highly expressed in connective tissue and has the ability to regulate collagen fibril assembly. Previous studies have shown that lumican is involved in wound healing, but the precise effects of lumican on reepithelialization and wound contraction, the two pivotal aspects of skin wound healing, have not been investigated. Here we explored the roles of lumican in fibroblast contractility, a main aspect of skin wound healing, by adopting mice skin wound healing model and the corresponding in vitro cellular experiments. Our results showed that lumican can promote skin wound healing by facilitating wound fibroblast activation and contraction but not by promoting keratinocyte proliferation and migration. Silencing of integrin α2 completely abolished the pro-contractility of lumican, indicating lumican enhances fibroblast contractility via integrin α2. Our study for the first time demonstrated that lumican can affect fibroblast's mechanical property, which is pivotal for many important pathological processes, such as wound healing, fibrosis, and tumor development, suggesting that lumican might have a potential to be used to modulate these processes. © 2013 Liu et al.
Li M.,Central Hospital of Fengxian District |
Han Y.,Central Hospital of Fengxian District |
Feng Y.C.,Central Hospital of Fengxian District
Journal of International Medical Research | Year: 2012
To compare transumbilical single-port laparoscopic hysterectomy (TSPLH) with traditional four-port total laparoscopic hysterectomy (TLH). Methods: Patients with benign uterine disease were assigned to receive either TSPLH (n = 52) or TLH (n = 56). Duration of surgery, intraoperative blood loss, conversion rate, time to first flatus, duration of immobilization, post -operative analgesia requirement, port site infection, port hernia, duration of hospital stay, postoperative fever rate and percentage patient satisfaction were recorded. Results: TSPLH and TLH were both performed successfully. TSPLH was associated with significantly longer duration of surgery, shorter duration of immobilization, lower rate of port site infection and higher patient satisfaction than TLH. There were no other significant differences between the two groups. All subjects recovered fully and no postoperative complications occurred during a 6-month (minimum) follow-up period. Conclusions: TSPLH was found to be a feasible and safe approach for laparoscopic hysterectomy. © 2012 Field House Publishing LLP.
Li R.-K.,Shanghai JiaoTong University |
Li R.-K.,Nanjing Medical University |
Zhao W.-Y.,Shanghai JiaoTong University |
Fang F.,Shanghai JiaoTong University |
And 10 more authors.
Journal of Cancer Research and Clinical Oncology | Year: 2014
Background: Lysyl oxidase-like 4 (LOXL4) has been found up-regulated in a variety of human malignancies, but its clinical significance and functional roles in gastric cancer (GC) remain unknown.Methods: Lysyl oxidase-like 4 (LOXL4) expression level in tumor tissues and human GC cell lines was evaluated by quantitative real-time polymerase chain reaction, Western blotting and immunohistochemical analyses. Its clinical significance was inferred from the analysis of 379 tissue samples of patients with GC using tissue microarray. The roles of LOXL4 in cell proliferation, migration and invasion in vitro were analyzed by gene over-expression, RNA interference and recombinant protein. Effects of LOXL4 on regulation of focal adhesion kinase/Src kinase (FAK/Src) pathway were examined by Western blotting.Results: Lysyl oxidase-like 4 (LOXL4) was up-regulated in GC tissues relative to paired non-tumor tissues, and this over-expression was significantly associated with tumor size, depth of tumor invasion, lymph node metastasis, tumor-node-metastasis (TNM) stages and poorer overall survival. Over-expression of LOXL4 has promotive effects on GC cell proliferation, migration and invasion in vitro, consistent with this, LOXL4 knockdown has inhibitive effects on GC cell proliferation, migration and invasion. Furthermore, recombinant human LOXL4 protein also promoted GC cell proliferation and migration. Subsequent mechanistic studies showed that LOXL4 could activate FAK/Src pathway to enhance cell–extracellular matrix adhesion.Conclusions: Taken together, our data reveal that up-regulation of LOXL4 expression is a frequent event in GC progression, contributes to tumor cell proliferation and metastasis, and LOXL4 may be a potential independent prognostic marker and therapeutic target for GC. © 2014, Springer-Verlag Berlin Heidelberg.
Zhang X.,Central Hospital of Fengxian District |
Wu L.,Fudan University |
Sheng Y.,Central Hospital of Fengxian District |
Zhou W.,Central Hospital of Fengxian District |
And 5 more authors.
Molecular Biology Reports | Year: 2012
Abstract Epidemiological studies found inconsistent results on the association of two variants on TGFBR1 (TGFBR1*6A and Int7G24A) with colorectal cancer (CRC) risk. The present study was aimed to evaluate the association of these two variants with CRC susceptibility via the meta-analysis methods. For variant TGFBR1*6A, nine reports including 6,765 CRC patients and 8,496 unrelated controls were identified. The heterozygotes *6A/ *9A showed a significant increased risk of CRC with the pooled OR was 1.12 (95% CI = 1.02-1.23), and the pooled OR for the homozygotes *6A/*6A was 1.13 (95% CI = 0.80-1.58) compared to the homozygotes *9A/*9A. However, under the dominant effect model, the TGFBR1*6A carriers showed a significantly increased CRC risk (pooled OR = 1.12, 95% CI = 1.03-1.23, *6A/ *6A and *6A/*9A vs. *9A/*9A). For variant Int7G24A, three case-control studies with 1,074 cases and 1,945 controls were found. Although no significant association was found for heterozygosity Int7G24A carriers with CRC risk (pooled OR = 0.97, 95% CI = 0.67-1.42), the homozygosity A/A carriers showed a significant elevated risk of CRC (pooled OR = 1.68, 95% CI = 1.14-2.47) compared to G/G homozygotes. Under the recessive effect model, homozygotes A/A showed a 71% increase of CRC risk compared to the A/G and G/G genotype carriers (pooled OR = 1.71, 95% CI = 1.17-2.51). These data strongly suggested that the two polymorphisms of TGFBR1 may confer low-penetrance susceptibility of CRC risk. © Springer Science+Business Media B.V. 2011.
PubMed | Central Hospital of Fengxian District
Type: Journal Article | Journal: European review for medical and pharmacological sciences | Year: 2015
DESs have been proved to be beneficial for patients with chronic total coronary occlusions (CTO) in terms of cardiac function and other prognosis. We aim to compare the efficacy and safety of drug-eluting stent (DES) and bare-metal stent (BMS) in CTO recanalization at different follow-up duration.Articles comparing outcomes between DES and BMS implantation in patients with CTO was searched. A fixed-effect (inverse-variance weighted) and random-effect (DerSimonian and Laird) model were used to analyze the pooling results.A total of 29 comparative studies including 24 cohort studies and 5 randomized controlled studies were identified with a total of 9140 patients (5008 received BMS and 4132 received DES). The risk of all cause death for DES was higher at 6 months and lower at 12 months than BMS, and no significant difference was shown at 24, 36 and 60 months. DES group had lower risk of MI after 12 months implantation, and no difference was shown at 6, 24, 36 and 60 months. Major adverse cardiovascular event (MACE)-free survival was clinically and significantly improved by 73%, 68%, 49%, 40% and 37% respectively in DES group at 6,12, 24, 36, and 60 months.DES is superior to BMS in binary restenosis, reocclusion and MACE-free survival during long-term follow up. The occurrences of all-cause death and MI show that the risk rate of BMS is higher than that of DES at 12 months. The frequency of all-cause death of DES is higher than BMS at 6 months. DES has higher risk of in-stent thrombosis than BMS at 36 months of implantation.