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Sun W.,Central Hospital of Fengxian District
Chinese Traditional and Herbal Drugs | Year: 2012

Objective To study the chemical constituents of Phyllanthus amarus. Methods Chromatography on silica gel column, Sephadex LH-20 columm, recrystallization, and preparative HPLC technique were used to isolate and purify the compounds. Spectroscopic methods including UV, IR, EI-MS, 1H-NMR, 13C-NMR, HMQC, and HMBC were used to elucidate the structures of compounds. Results Nine compounds were obtained and identified as palmitic acid (1), 4-hydrobenzal dehyde (2), oleanolic acid (3), stigmast-5-en-3-ol, oleate (4), 4-hydroxy-3-methoxy-benzoic acid (5), daucosterol (6), apigenin (7), luteolin (8), and phyllanoside (9). Conclusion Compound 9 is a new compound, named phyllanoside. Compounds 4-8 are isolated from the plant for the first time. Source


Li R.-K.,Shanghai JiaoTong University | Li R.-K.,Nanjing Medical University | Zhao W.-Y.,Shanghai JiaoTong University | Fang F.,Shanghai JiaoTong University | And 10 more authors.
Journal of Cancer Research and Clinical Oncology | Year: 2014

Background: Lysyl oxidase-like 4 (LOXL4) has been found up-regulated in a variety of human malignancies, but its clinical significance and functional roles in gastric cancer (GC) remain unknown.Methods: Lysyl oxidase-like 4 (LOXL4) expression level in tumor tissues and human GC cell lines was evaluated by quantitative real-time polymerase chain reaction, Western blotting and immunohistochemical analyses. Its clinical significance was inferred from the analysis of 379 tissue samples of patients with GC using tissue microarray. The roles of LOXL4 in cell proliferation, migration and invasion in vitro were analyzed by gene over-expression, RNA interference and recombinant protein. Effects of LOXL4 on regulation of focal adhesion kinase/Src kinase (FAK/Src) pathway were examined by Western blotting.Results: Lysyl oxidase-like 4 (LOXL4) was up-regulated in GC tissues relative to paired non-tumor tissues, and this over-expression was significantly associated with tumor size, depth of tumor invasion, lymph node metastasis, tumor-node-metastasis (TNM) stages and poorer overall survival. Over-expression of LOXL4 has promotive effects on GC cell proliferation, migration and invasion in vitro, consistent with this, LOXL4 knockdown has inhibitive effects on GC cell proliferation, migration and invasion. Furthermore, recombinant human LOXL4 protein also promoted GC cell proliferation and migration. Subsequent mechanistic studies showed that LOXL4 could activate FAK/Src pathway to enhance cell–extracellular matrix adhesion.Conclusions: Taken together, our data reveal that up-regulation of LOXL4 expression is a frequent event in GC progression, contributes to tumor cell proliferation and metastasis, and LOXL4 may be a potential independent prognostic marker and therapeutic target for GC. © 2014, Springer-Verlag Berlin Heidelberg. Source


Zhu J.,Shanghai JiaoTong University | Zhao Y.,Central Hospital of Fengxian District | Liu F.,Central Hospital of Fengxian District | Huang Y.,Shanghai JiaoTong University | And 2 more authors.
BMC Musculoskeletal Disorders | Year: 2012

Background: To define the sites where the lateral femoral cutaneous nerve (LFCN) is more easily visualized and to describe the anatomical variations of the LFCN. Methods. A total of 240 LFCNs in 120 volunteers were evaluated with 18 MHz ultrasound; the intermuscular space between the tensor fasciae latae muscle and the sartorius was used as an initial sonographic landmark. The time taken to identify the nerve was recorded. The number of nerve branches at the level of the inguinal ligament (IL) and the relationship between the LFCN and the IL was assessed. The nerve cross-sectional area (CSA) of the LFCN and the distance between the LFCN and the anterior superior iliac spine was measured. Results: Each nerve was identified using ultrasound in all participants. The mean time for identifying the nerve was 7s for unilateral LFCNs. The nerve passed under the IL in 198 cases, whereas in 44 cases, it passed through to the IL. The LFCN consisted of 1-4 branches just after its passage under or through the IL. The CSA of the LFCN was 1.04±0.44 mm§ssup§2§esup§, and the mean distance between the LFCN and the anterior superior iliac spine was 15.6 ± 4.2 mm. Conclusions: It is easier to identify the LFCN if the intermuscular space between the tensor fasciae latae muscle and the sartorius is used as an initial sonographic landmark. The anatomical variation of the LFCN can be viewed with high-frequency ultrasound. © 2012 Zhu et al.; licensee BioMed Central Ltd. Source


Liu X.-J.,Shanghai JiaoTong University | Kong F.-Z.,Shanghai JiaoTong University | Wang Y.-H.,Shanghai JiaoTong University | Zheng J.-H.,Shanghai JiaoTong University | And 9 more authors.
PLoS ONE | Year: 2013

Lumican is a dermatan sulfate proteoglycan highly expressed in connective tissue and has the ability to regulate collagen fibril assembly. Previous studies have shown that lumican is involved in wound healing, but the precise effects of lumican on reepithelialization and wound contraction, the two pivotal aspects of skin wound healing, have not been investigated. Here we explored the roles of lumican in fibroblast contractility, a main aspect of skin wound healing, by adopting mice skin wound healing model and the corresponding in vitro cellular experiments. Our results showed that lumican can promote skin wound healing by facilitating wound fibroblast activation and contraction but not by promoting keratinocyte proliferation and migration. Silencing of integrin α2 completely abolished the pro-contractility of lumican, indicating lumican enhances fibroblast contractility via integrin α2. Our study for the first time demonstrated that lumican can affect fibroblast's mechanical property, which is pivotal for many important pathological processes, such as wound healing, fibrosis, and tumor development, suggesting that lumican might have a potential to be used to modulate these processes. © 2013 Liu et al. Source


Zhou W.Q.,Tongji University | Sheng Q.Y.,Central Hospital of Fengxian District | Sheng Y.H.,Central Hospital of Fengxian District | Hou W.J.,Central Hospital of Fengxian District | And 3 more authors.
European Journal of Gynaecological Oncology | Year: 2015

Purpose of investigation: To explore the significance of survivin, P16INK4a, COX-2, and Ki-67 expressions for prediction of cervical cancer progression. Materials and Methods: A retrospective study was performed in 129 cases including 24 squamous carcinoma of the cervix (SCC), 70 cervical intraepithelial neoplasias (CIN), 15 cervical condyloma acuminatum (CCA), ten chronic cervicitis (CC), and ten normal cervix (NC). Protein expressions were evaluated using immunohistochemistry. Results: Survivin, P161NK4a; COX-2, and Ki67 were highly expressed in SCC and CIN compared with others. Their expression rates were gradually increased in CIN I, CIN II, CIN III, and SCC groups, showing 72.00%, 88.00%, 90.00%, and 95.83% for P16INK4a, 68.00%, 84.00%, 95.00% and 100.00% for COX-2, 76.00%, 96.00%, 100.00%, and 100.00 for Ki-67, respectively. There were significant correlations between survivin and P16INK4a, COX-2, Ki-67, as well as P16INK4a and Ki-67. Conclusion: Survivin, P16INK4a, COX-2 and Ki-67 play critical roles for development and progression of cervical cancer. Source

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