Bharathi L.K.,Central Horticultural Experiment Station IIHR |
Vinod,Indian Agricultural Research Institute |
Ghosh N.,West Texas A&M University |
Behera T.K.,Indian Agricultural Research Institute |
And 2 more authors.
African Journal of Agricultural Research | Year: 2011
Interspecific hybrid (F 1) between spine gourd (Momordica dioica) and teasle gourd (Momordica subangulata Blume subsp. Renigera (G. Don) WJJ de Wilde) produced through bagging and hand pollination showed vigorous growth habit with less fertility. Morphological parameters along with meiotic, mitotic chromosome behaviors and Randomly Amplified Polymorphic DNA (RAPD) analysis, confirmed interchanges of genetic characters in the new hybrids. The interspecific hybrid showed a vigorous foliage with intermediate flower characters than that of parental species. Self pollination and backcrossing of F 1 plants to either parent confirmed presence of both male and female sterility, that might be due to improper meiotic chromosome pairing. The characters like petal spot and time of anthesis (early morning) were similar to that of pollen parent while the diameter and size of leaves and flowers, pedicel length and plant growth were intermediate as compared to parents. Somatic chromosome analysis revealed 2n = 28 in M. dioica - the female parent, 2n = 56 in M. subangulata ssp. renigera - the male parent and 2n = 42 in F 1 hybrid of M. dioica × M. subangulata ssp. renigera. RAPD analysis of both the parents and F 1 hybrid confirmed more genetic affinity (60.95%) with female parent than the male parent (32.35%). Primer specific DNA markers confirmed the introgression of male genetic elements into the newly developed amphidiploids. Incorporation of male specific DNA markers into the F 1 hybrid is discussed. Preliminary evaluation indicates that the F 1 plants sprouted early, which is lacking in the mother parent and this character could be exploited by restoring of fertility by chromosome doubling. © 2011 Academic Journals.
Karunakaran G.,Central Horticultural Experiment Station IIHR |
Ravishankar H.,Central Horticultural Experiment Station IIHR |
Ravishankar H.,Central Institute for Sub tropical Horticulture |
Sakthivel T.,Central Horticultural Experiment Station IIHR |
And 3 more authors.
Indian Journal of Horticulture | Year: 2014
In the present study, 45- and 60-day-old Coorg mandarin micro-buds were budded on five- and six-month-old Rangpur lime rootstock seedlings. Higher success of micro-budded plants was recorded (30.71%) on six-month-old Rangpur lime rootstocks by using 60-day-old scion buds, which was higher than the five-month-old rootstock seedlings (18.66%) with same age of scion buds. Further, in order to increase the success, microbudding was attempted under protected structures. Success of micro-budding on Rangpur lime was higher in polyhouse (56.49%) than shade nethouse (34.44%) and open conditions (21.50%) than other rootstocks. The number of days taken for sprouting was much earlier under protected conditions in Rangpur lime (13.36%) followed by Troyer citrange (14.82%) and trifoliate orange (19.54%) rootstocks. Micro-budded plants under open conditions took longer time for sprouting on Rangpur lime (18.32). Success of micro-budding was found to be significantly higher in protected structures than the open conditions for all the rootstocks. The results suggested that micro-budding technique could be gainfully exploited for shortening the propagation period and early biological indexing of citrus. © 2014, Horticulture Society of India. All rights reserved
Kumar D.,Central Horticultural Experiment Station IIHR |
Pandey V.,IIVR |
Nath V.,NRC on Litchi
Indian Journal of Horticulture | Year: 2012
A field experiment was conducted at Central Horticultural Experiment Station, Bhubaneswar during 2007-08 to assess the effects of NPK fertigation on growth, yield and quality of vegetable banana Monthan (Banthal- ABB). There were five treatments viz., T1 - Recommended dose of fertilizer (RDF - 200 g N: 80 g P: 220 g K /plant/crop cycle), T2 - 75% RDF (150 g N: 60 g P: 155 g K /plant/crop cycle)+ application schedule-I (Application of N:P:K in the ratio of 2:1:0 at vegetative growth, 0:2:1 at flowering stage and 1:0:2 at fruit development and maturation stage), T3- 75% RDF + application schedule-II (Application of N:P:K in the ratio of 3:2:1 at vegetative growth, 1:3:2 at flowering stage and 2:1:3 at fruit developmental and maturation stage), T4 - 50% RDF (100 g N: 40 g P: 110 g K/plant/crop cycle) + application schedule - I and T5 - 50% RDF+ application schedule-II laid out in randomized block design with 5 replications. Maximum pseudo-stem height (261.50 cm), stem circumference (65.75 cm), number of hands and fingers (7.20 and 70.12/plant), bunch weight (11.45 kg/plant) and fruit yield (28.63 t/ha), finger size (20.50 × 15.65 cm), finger weight (163.29 g), total leaf area (19.88 m2), productivity efficiency (0.575 kg/m2 leaf area) and leaf, fruit and pseudo-stem dry matter (33.45, 15.27 and 9.98%) and leaf nutrient content (N-2.21%, P-0.26% and K-1.98%) and TSS (6.75°B) were recorded due to application of 75% recommended dose of fertilizers + application schedule-II (Applied N:P:K in the ratio of 3:2:1 at vegetative growth, 1:3:2 at flowering stage and 2:1:3 at fruit development stage). Earliest flowering and fruit maturity (12.41 and 17.91 days) were also recorded in the same treatment. Pulp: peel ratio (2.68) was maximum due to 50% RDF + application schedule-II (applied 3:2:1 at vegetative growth, 1:3:2 at flowering stage and 2:1:3 at fruit development and maturation stage) in vegetable banana Monthan (ABB-Banthal).
Kishore K.,Central Horticultural Experiment Station IIHR |
Kishore K.,Indian Institute of Horticultural Research |
Singh H.S.,Central Horticultural Experiment Station IIHR |
Singh H.S.,Indian Institute of Horticultural Research |
And 2 more authors.
Indian Journal of Agricultural Sciences | Year: 2015
Paclobutrazol (PBZ), a triazole derivative, has been effectively used to induce and manipulate flowering, fruiting and tree vigour in several perennial fruit crops. However its use in mango is quite common. Soil application of paclobutrazol has been efficacious in promoting flowering and increasing yield in many fruit crops. However, there are some conflicting reports on its impact on fruit quality parameters. Besides reducing gibberellins level, PBZ increases cytokinin contents, root activity and C: N ratio, whereas its influence on nutrient uptake lacks consistency. PBZ also affects microbial population and dehydrogenase activity in soil. PBZ has been characterized as an environmentally stable compound in soil and water environments with a half-life of more than a year under both aerobic and anaerobic conditions. However, its residue could not be detected above quantifiable level (0.01 ppm) in soils and fruits when applied in optimized rate. The potential of PBZ to contaminate groundwater at optimum concentrations is low however the risk of its exposure to aquatic life is high. PBZ is considered moderately hazardous for human beings with remote chance of being genotoxic and carcinogenic. In view of the above, optimized use of the PBZ to derive maximum benefit with least undesirable impact on food and environmental safety aspects is suggested.