Venugopala Reddy K.R.,P.A. College |
Anantha Ram G.,Central Drugs Testing Laboratory |
Harish M.N.K.,Kuvempu University |
Arthob Naik Y.,Kuvempu University |
Keshavayya J.,Kuvempu University
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2012
A simple, fast, precise and accurate rapid reverse phase high-performance liquid chromatography (HPLC) method was developed for the quantitative determination of tegaserod maleate in bulk & solid dosage formulations. The drug was chromatographed on (SUPLEX PKB-100) column. Eluents were monitored at a wavelength 277 nm using a mixture of acetonitrile and 0.05 M potassium phosphate buffer pH 3.0, consisting of 0.005 M 1-Octanesulphonic acid sodium salt, methanol in the ratio of 50:22:28. A linear response (r = 0.9999) was observed in the range of 25μg/ml-75μgmL-1. The proposed method is sufficiently selective to distinguish the parent drug and the degradation products after hydrolysis, photolysis, or chemical oxidation and from excipients .The method showed good recoveries (average 99.45%) and the relative standard deviation ≤ 1.0%. The developed method can be used for routine quality control analysis of tegaserod maleate in solid dosage as well as bulk formulations.