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Yoo S.Y.,Forensic Center | Yoo S.Y.,Kongju National University | Cho N.S.,Central District Office | Park S.W.,Kongju National University | And 4 more authors.
Forensic Science International: Genetics Supplement Series | Year: 2011

We have examined 45 Korean haplotype transfers in 41 families at eight X-linked STRs (DXS10135 and DXS8378 in linkage group 1, DXS7132 and DXS10074 in linkage group 2, HPRTB and DXS10101 in linkage group 3, DXS10134 and DXS7423 in linkage group 4) of Mentype Argus X-8 kit. The allele frequencies and haplotype frequencies within the four linkage groups were determined for females and males, respectively. Deviation from Hardy-Weinberg expectation could not be detected (p < 0.05). No recombination was observed within each 4 linkage groups, however, six cases of mutation were founded in DXS10135, DXS10101, and DXS10134. Details of X-STR haplotype study in Koreans would be useful in kinship testing and forensic applications. © 2011 Elsevier Ireland Ltd. Source


Choi Y.H.,Yeungnam University | Zhou W.,Yeungnam University | Oh J.,Yeungnam University | Choe S.,Southern District Office | And 3 more authors.
Bioorganic and Medicinal Chemistry Letters | Year: 2012

In spite of the critical role of the natural products in drug discovery, surprising little attention has been placed on endangered and rare plant species that could play a pivotal role in pharmaceutical and fiber development. Protein tyrosine phosphatase-1B (PTP1B), which blocks insulin signaling, has been gaining interest to be a promising therapeutic target for the treatment of type 2 diabetes mellitus. Bioassay-guided fractionation on the leaves of Rhododendron brachycarpum G. Don (Ericaceae) yielded seven PTP1B inhibitory triterpenoids, including a new triterpene, rhododendric acid A (1). Their PTP1B inhibitory potency and their lipophilicity were investigated to provide a feasible scaffold that may overcome the innate limitation of the previously reported PTP1B inhibitors. © 2012 Elsevier Ltd. All rights reserved. Source


Choe S.,Southern District Office | Woo S.H.,Central District Office | Kim D.W.,Central District Office | Park Y.,Southern District Office | And 4 more authors.
Analytical Biochemistry | Year: 2012

After gas chromatography-mass spectrometry (GC-MS) analysis, data processing, including retention time correction, spectral deconvolution, peak alignment, and normalization prior to statistical analysis, is an important step in metabolomics. Several commercial or free software packages have been introduced for data processing, but most of them are vendor dependent. To design a simple method for Agilent GC/MS data processing, we developed an in-house program, "CompExtractor", using Microsoft Visual Basic. We tailored the macro modules of an Agilent Chemstation and implanted them in the program. To verify the performance of CompExtractor processing, 30 samples from the three species of the genus Papaver were analyzed with Agilent 5973 MSD GC-MS. The results of CompExtractor processing were compared with those of AMDIS-SpectConnect processing by hierarchical cluster analysis (HCA) and principal component analysis (PCA). The two methods showed good classification according to their species in HCA. The PC1 + PC2 scores were 54.32-63.62% for AMDIS-SpectConnect and 56.65-85.92% for CompExtractor in PCA. Although the CompExtractor processing method is an Agilent GC-MS-specific application and the target compounds must be selected first, it can extract the target compounds more precisely in the raw data file with batch mode and simultaneously assemble the matrix text file. © 2012 Elsevier Inc. All rights reserved. Source


Cho H.E.,Chungbuk National University | Ahn S.Y.,Central District Office | Kim D.-W.,Central District Office | Woo S.-H.,Central District Office | And 4 more authors.
Biomedical Chromatography | Year: 2014

A sensitive and specific high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the determination of Grayanotoxin I (GTX I) and Grayanotoxin III (GTX III) in rat whole blood. Grayanotoxins (GTXs) and clindamycin as internal standard (IS) were extracted from rat blood via solid-phase extraction using PEP solid-phase extraction cartridges. Chromatographic separation of the analytes was achieved on a Kinetex C18 (100×2.1mm, 2.6μm) reversed-phase column using a gradient elution with the mobile phase of 1% acetic acid in water and methanol at a flow rate of 0.2mL/min. Electrospray ionization mass spectrometry was operated in the positive ion mode with multiple reaction monitoring. The calibration curves obtained were linear over the concentration range of 1-100ng/mL with a lower limit of quantification of 1ng/mL for GTXs. The relative standard deviation of intra-day and inter-day precision was below 6.8% and accuracy ranged from 94.8 to 106.6%. The analytes were stable in the stability studies. The validated method was successfully applied to the quantification and toxicokinetic study of GTXs in rats for the first time after oral administration of 11.52mg/kg mad honey and 0.35mg/kg GTX III, respectively. © 2014 John Wiley & Sons, Ltd. Source


Yoo S.Y.,Central District Office | Yoo S.Y.,Kongju National University | Cho N.S.,Central District Office | Park M.J.,Yonsei University | And 7 more authors.
Molecules and Cells | Year: 2011

Genotyping of highly polymorphic short tandem repeat (STR) markers is widely used for the genetic identification of individuals in forensic DNA analyses and in paternity disputes. The National DNA Profile Databank recently established by the DNA Identification Act in Korea contains the computerized STR DNA profiles of individuals convicted of crimes. For the establishment of a large autosomal STR loci population database, 1805 samples were obtained at random from Korean individuals and 15 autosomal STR markers were analyzed using the AmpFlSTR Identifiler PCR Amplification kit. For the 15 autosomal STR markers, no deviations from the Hardy-Weinberg equilibrium were observed. The most informative locus in our data set was the D2S1338 with a discrimination power of 0.9699. The combined matching probability was 1.521 × 10 -17. This large STR profile dataset including atypical alleles will be important for the establishment of the Korean DNA database and for forensic applications. © 2011 The Korean Society for Molecular and Cellular Biology and Springer Netherlands. Source

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