Time filter

Source Type

Kamal Y.,Hamdard University | Mohammed Musthaba S.,Hamdard University | Singh M.,Hamdard University | Parveen R.,Hamdard University | And 6 more authors.
Biomedical Chromatography | Year: 2012

An attempt has been made to develop and validate a simultaneous HPLC method for novel approach of drug release via oil-in-water (o/w) nanoemulsion formulation and Habb-e-Khardal Unani tablet containing piperine and guggul sterones E and Z as main ingredients. Nanoemulsion was prepared by titration method using sefsol-218 as an oily phase, cremophor-EL as a surfactant, transcutol as a co-surfactant and distilled water as an aqueous phase. The formulation was optimized on the basis of thermodynamic stability and dispersibilty test. The nanoformulation was evaluated for particle size, surface morphology, electrical conductivity and viscosity determination. The in vitro dissolution was carried out by dialysis bag method. Drugs were quantified using an HPLC method developed in-house with a C18 column as stationary phase and acetonitrile and water as mobile phase at λmax of 240nm. The optimized formulation showed higher drug release, lower droplet size and less viscosity as compared with the conventional Habb-e-Khardal Unani tablet. The present study illustrated the potential of nanoemulsion dosage form in improving biopharmaceutic performance of piperine and guggul sterone. The HPLC method was also found to be quite sufficient for the routine quality control of formulations containing piperine and guggul sterone E and Z as ingredients and also for in vitro drug release studies. © 2011 John Wiley & Sons, Ltd.


Abbas Zaidi S.M.,P.A. College | Jameel S.S.,Central Council for Research in Unani Medicine | Jameel S.S.,Hamdard University | Zaman F.,Hamdard University | And 3 more authors.
Alternative Medicine Review | Year: 2011

Leeches are a class of segmented invertebrates, known for their blood-feeding habits and used in phlebotomy to treat various ailments since antiquity. In Europe, medicinal leeches have recently been rediscovered and are used by maxillofacial and other microsurgeons to aid salvage of compromised venous engorged tissue and amputations, such as digits, ears, and nasal tips. Because of their important salivary components, blood-sucking (sanguivorous) leeches, such as Hirudo medicinalis and related species, have engendered great interest from pharmaceutical companies searching for anticoagulants to prevent blood clotting during microsurgeries. Scientific research reveals that the beneficial effects of leeching, in addition to decongestion, include injection of a cocktail of several medicinally useful bioactive molecules present in their saliva. Owing to its therapeutic potential, the research is continuing as many new salivary compounds are being isolated and synthesized. Copyright © 2011 Alternative Medicine Review, LLC. All Rights Reserved.


Akbar S.,Regional Research Institute of Unani Medicine | Khan S.A.,Central Council for Research in Unani medicine | Masood A.,University Of Kashmir | Iqbal M.,Regional Research Institute of Unani Medicine
African Journal of Traditional, Complementary and Alternative Medicines | Year: 2010

Some plants used in Unani system of medicine are toxic, even deadly poisonous. The drugs having such plants as their components are detoxified before they are dispensed to the patients. One such drug, capsule Hudar, has Strychnos nux-vomica L. (Azraqi) seeds as one of its components and is very effectively used to elevate blood pressure. Ancient manuscripts describe any methods of its detoxification. It has been found that the detoxification processes studied reduce the strychnine content, as determined either by using uv-vis spectrophotometer or HPLC, present in Strychnos nux vomica seeds which is responsible for Strychnos nux vomica toxicity. The decrease in strychnine amount was best when the seeds were immersed for detoxification in excess of water for 5 days, in milk for 2 days followed by their boiling in milk. Strychnine in small amounts has been reported to give subjective feeling of stimulation.


PubMed | Central Council for Research in Unani Medicine and University Of Kashmir
Type: | Journal: Scientifica | Year: 2016

Capsule Shaqeeqa, Unani formulation, is prescribed for the clinical treatment of diseases like sinusitis, headache, and migraine. The safety evaluation data of it is not available; in order to provide the safety data the present study was carried out. The study was carried out on four groups of rats (n = 5). Two groups (one male and one female group) as normal controls were orally given water while the other two groups were orally given daily doses of drug at the dose level of 150mg/kg of body weight for duration of 90 days. Physiological parameters like body weight, feed consumption, water consumption, and clinical signs were regularly monitored and recorded. Organs were collected, examined, and weighed and specimens were taken for histopathological studies. The results showed that the drug did not alter the physiological parameters. There was no mortality or any morbidity found in drug treated rats. There was no statistical significant change found in any haematological or biochemical parameter of rats orally fed with Shaqeeqa. A statistically insignificant association verified that haematological and biochemical parameters were rendered unaffected by the drug. Moreover histological investigations of essential key organs demonstrated that the drug did not prompt any histopathological change. These observations demonstrate the safety of Capsule Shaqeeqa at the studied dosage levels.


Bharti S.,All India Institute of Medical Sciences | Golechha M.,All India Institute of Medical Sciences | Kumari S.,Indian Agricultural Research Institute | Siddiqui K.M.,Central Council for Research in Unani Medicine | Arya D.S.,All India Institute of Medical Sciences
European Journal of Nutrition | Year: 2012

Purpose: Traditional medicine has been appropriately identified as the most productive soil for the cultivation and harvesting of modern medicines. Herein, we postulate that safranal, an active constituent of Crocus sativus, owing to its strong antioxidant and anti-apoptotic potential, could be a valuable molecule in alleviating myocardial ischemia-reperfusion (IR) injury. Methods: To evaluate this hypothesis, safranal (0.1-0.5 mL/kg/day, i.p.) or saline were administered to rats for 14 days, and on 15th day, one-stage ligation of left anterior descending coronary artery for 45 min was performed, followed by 60 min reperfusion. Results: We concluded that safranal not only significantly decreased infarct size, but also improved left ventricular functions and the overall hemodynamic status of the myocardium. Interestingly, safranal enhanced phosphorylation of Akt/GSK-3β/eNOS and suppressed IKK-β/NF-κB protein expressions in IR-challenged myocardium. Our findings also imply that safranal exhibits strong anti-apoptotic potential, as evidenced by upregulated Bcl-2 expression and downregulated Bax and caspase3 expression with decreased TUNEL positivity. Moreover, safranal dose-dependently normalized myocardial antioxidant and nitrotyrosine levels, cardiac injury markers (LDH and CK-MB), and decreased TNF-α level in IR-insulted myocardium. Histopathological and ultrastructural findings correlated with the functional and biochemical outcomes showing preserved myocardial architecture and decreased inflammatory cells and edema. Conclusions: Taken together, these results provide convincing evidence of safranal as an invaluable molecule in myocardial IR setting probably due to its fortified antioxidant and anti-apoptotic potential. © 2011 Springer-Verlag.


Sachdeva J.,All India Institute of Medical Sciences | Tanwar V.,All India Institute of Medical Sciences | Golechha M.,All India Institute of Medical Sciences | Siddiqui K.M.,Central Council for Research in Unani Medicine | And 4 more authors.
Experimental and Toxicologic Pathology | Year: 2012

Saffron (dried stigmas of Crocus sativus L.), a naturally derived plant product, has long been used as a traditional ancient medicine against various human diseases. The aim of the series of experiments was to systematically determine whether saffron exerts cardioprotection in isoproterenol-induced myocardial damage. Male Wistar rats (150-175. g) were divided into five groups: control, isoproterenol (ISO) and three saffron (200, 400 and 800. mg/kg) treatment groups. Aqueous extract of saffron or vehicle was administered orally to rats for four weeks. On days 28 and 29, the animals in ISO and saffron treatment groups were administered ISO (85. mg/kg, s.c.) at an interval of 24. h. On day 30, after recording hemodynamics and left ventricular functions, animals were sacrificed for biochemical, histopathological and electromicroscopical examinations. Isoproterenol challenged animals showed depressed hemodynamics and left ventricular functions as evident by decreased left ventricular rate of peak positive and negative pressure change and elevated left ventricular end-diastolic pressure. Structural and ultrastructural studies further confirmed the damage which was reconfirmed by increased thiobarbituric acid reactive substances (p<. 0.001) and decreased creatine kinase-MB and lactate dehydrogenase (p<. 0.001). In addition, significant reduction in superoxide dismutase and catalase (p<. 0.001) was observed in ISO group. Our results suggested that saffron at all the doses exerted significant cardioprotective effect by preserving hemodynamics and left ventricular functions, maintaining structural integrity and augmenting antioxidant status. Among the different doses used, saffron at 400. mg/kg dose exhibited maximum protective effects which could be due to maintenance of the redox status of the cell reinforcing its role as an antioxidant. © 2010 Elsevier GmbH.


Goyal S.,All India Institute of Medical Sciences | Siddiqui M.K.,Central Council for Research in Unani Medicine | Siddiqui K.M.,Central Council for Research in Unani Medicine | Arora S.,All India Institute of Medical Sciences | And 3 more authors.
Experimental and Toxicologic Pathology | Year: 2010

The present study was designed to investigate whether Khamira Abresham Hakim Arshad Wala (KAHAW), a preparation of Unani System of Medicine, is able to attenuate the isoproterenol (ISO)-induced myocardial necrosis on the basis of its effects on hemodynamic, antioxidant, histopathological and ultrastructural parameters. Male Wistar albino rats were administered KAHAW (200, 400 and 800 mg/kg/day, orally) or vehicle for 14 days with concurrent ISO administration (85 mg/kg, subcutaneously, 2 doses at 24 h interval) on 13th and 14th day. On the 15th day, vehicle+ISO-treated rats exhibit cardiac dysfunctions as indicated by decrease in systolic, diastolic, and mean arterial pressures, reduction in both maximum positive and maximum negative rates of developed left ventricular pressure (±LVdp/dt) and an increase in left ventricular end-diastolic pressure (LVEDP). Biochemical analysis of their heart homogenate presented reduced levels of enzymes viz., superoxide dismutase (SOD), catalase (CAT), lactate dehydrogenase (LDH), creatine kinase-MB (CK-MB) isoenzyme. A marked reduction in reduced glutathione (GSH) levels along with increase in levels of thiobarbituric acid reactive substances (TBARS) was also observed in rat myocardium. Myocardial necrosis, edema and inflammation were evident from the light microscopic and ultrastructural changes. KAHAW at dose of 800 mg/kg/day significantly reversed majority of hemodynamic and antioxidant derangements. The protective role of KAHAW on ISO-induced myocardial necrosis was further confirmed by histopathological and ultrastructural examination. There was no significant change in heart rate in all experimental groups. KAHAW per se groups showed no significant change when compared with vehicle control group. The study results thus demonstrated the cardioprotective potential of KAHAW against ISO-induced myocardial necrosis and associated oxidative stress. © 2009 Elsevier GmbH. All rights reserved.


Singh V.K.,Central Council for Research in Unani Medicine | Shweta,Ds College Affiliated To Dr Br Ambedar University
Contemporary Folk Medicines of India to Combat Human and Veterinary Diseases and Conditions | Year: 2012

Ethno-medicines have gained new dimensions in the present days through phyto-chemical research in India and abroad. Information on medicinal plants and folk drugs recorded during fieldwork is now being subjected to investigation in the search for new biodynamic compounds of therapeutic value. The curative properties of plants acquired by trial and error over centuries in "Human Laboratories" of tribals hold high esteem all over the globe. The present study is based on this rationale and provides first-hand information on some 1120 contemporary ethno-pharmacological prescriptions involving 444 taxa of medicinal plants collected and identified from certain rich tribal inhabited forest zones of the country between 1971-1997. © 2012 by Nova Science Publishers, Inc. All rights reserved.


Gupta P.,All India Institute of Medical Sciences | Mehla J.,All India Institute of Medical Sciences | Siddique K.,Central Council for Research in Unani Medicine | Jamil S.,Central Council for Research in Unani Medicine | Gupta Y.K.,All India Institute of Medical Sciences
Indian Journal of Traditional Knowledge | Year: 2015

Inflammation and oxidative stress have been reported in obesity. Safoof Mohazzil, is a traditional formulation prescribed by Unani physicians for weight loss. In the present study, antioxidant and anti-inflammatory properties of Safoof Mohazzil were evaluated using pyrogallol induced hepatotoxicity and carrageenan induced paw edema, respectively, in male Wistar rats. For the antioxidant study, rats were treated with Safoof Mohazzil for 14 days at the doses of 250, 500 and 1000 mg/kg, p.o. On 14th day, 2 hrs after the last dose of Unani formulation, pyrogallol (100 mg/kg) was injected intraperitoneally and on next day the animals were sacrificed for estimation of hepatic oxidative stress markers (malondialdehyde and reduced glutathione). Safoof Mohazzil demonstrated dose-dependent anti-oxidant activity. To assess its anti-inflammatory property, Safoof Mohazzil was administered at the doses of 500 & 1000 mg/kg, p.o. for 7 days and on 7th day carrageenan was administered 1 hr after the last dose of Unani formulation. The change in paw volume was calculated at 1, 3 and 6 hrs. Significant anti-inflammatory activity was found at 1000 mg/kg dose which is the human equivalent of its anti-obesity dose. The antioxidant and anti-inflammatory activity of Safoof Mohazzil support its use in obesity and possibly metabolic syndrome. © 2015, National Institute of Science Communication and Information Resources (NISCAIR). All rights reserved.


Alam M.,Regional research Institute of Unani Medicine | Khan H.,Regional research Institute of Unani Medicine | Samiullah L.,Regional research Institute of Unani Medicine | Siddique K.M.,Central council for Research in Unani Medicine
Journal of Applied Pharmaceutical Science | Year: 2012

Oleo-gum resin of Boswellia serrata Roxb ex Colebr. (Burseraceae) called Kundur in Unani system of Medicine is a prime ingredient in modern quality perfumes. The gum is popularly used in Indian Systems of Medicine (Unani, Ayurvedic & Sidha) for the last several centuriesin curing various aliment especially rheumatism and skin diseases. Kundur is one of the popular drugs for various ailments such as dysentery, dyspepsia, lung diseases, haemorrhoids, rheumatism, urinary disorders and corneal ulcer in Unani system of medicine for the last several years. It is also an ingredient in certain compound formulations viz: Majoon Kundur, Majoon Murawwah-ul-Arwah, Dawa-ul-Kibrit and HabbeSuzak of Unani medicine used in renal disorders. The studies carried out on Kundur (Boswellia serrata Roxb) reveal that Oleo-gum resin exhibits potent Anti-fungal, Anti-complementary, Juvenomimetic and Anti-carcinogenic properties. Investigations on Kundur also revealed its beneficial effects in Immunomodulation, Bronchial asthma, Polyarthritis, Hepatitis C-virus, Colitis and Crohn's disease. Phytochemistry andpharmacology on Kundur (Oleo-gum resin) of Boswellia serrata Roxb has been reviewed in this paper with the view to justify its recorded uses in Unani System of Medicine on scientific lines.

Loading Central Council for Research in Unani Medicine collaborators
Loading Central Council for Research in Unani Medicine collaborators