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Abdel-Halim K.Y.,Central Agricultural Pesticides Laboratory | Abdel-Raouf M.,Menoufia University | Abdou G.Y.,National Research Center of Egypt
International Journal of ChemTech Research

The role of aqueous ozonation among stimulation of the potential toxic effects of herbicide; thiobencarb on mosquito fish Gambusia affinis was examined after 96 hrs exposure. Two levels (1/5 and 1/10LC50) of herbicide were used individuals or mixed with either 1 or 5 ppm of ozone (O3). The aqueous ozonation of thiobencarb stimulated acetylcholinestrase (AChE) activity to reach the highest value (0.186 μmole.mg-1.min-1) in case of [1/5LC50 thiobencarb+O3 (5 ppm)] treatment. All treatments induced increase of malondialdehyde (MDA) levels compared with untreated group which not exceeded than 1.52 mM.g-1 tissue. Catalase (CAT) and glutathione peroxidase (GPx) showed variability in their response to ozonation process, especially GPx which subjected to values lowest than those of untreated group. On the other hand, lactic dehydrogenase (LDH) activity increased in most treatments, where 1/10LC50 thiobencarb recorded the highest value (512.7 U. L-1). The lowest one was recorded for [1/10LC50 thiobencarb+O3 (1 ppm)] treatment to be 129.5 U. L-1. This fact represents the cytotoxic effect of examined herbicide under ozonation condition. The all data revealed that, ozonation process for pesticide removal must be done under especial conditions and usage of catalytic agents to decline their potential toxic effects on organisms. © 2016, Sphinx Knowledge House. All Rights Reserved. Source

Hussein I.H.,Fayoum University | Hashim E.F.,Fayoum University | Toson M.G.,Central Agricultural Pesticides Laboratory | Gnedy M.M.,Central Agricultural Pesticides Laboratory
Egyptian Journal of Biological Pest Control

All tested plant oils (cottonseed, linseed, castor, clove, onion, citronella, lemongrass and chamomile) were miscible with xylene. Addition polyethyleneglycol (9%) as emulsifier and xylene (11%) as co-solvent to 80% of crude plant oils was used in formulation process as emulsifiable concentrates. Also, all locally formulated plant oils were passed successfully in all physiochemicals parameters i.e. emulsion stability, cold test, free acidity and alkalinity tests. All tests were carried out according to the international specifications by CIPAC, WHO and FAO. Obtained results indicated that reducing the surface tension and pH value with increasing electrical conductivity led to enhancement in wetting, spreading and retention of plant oils used as spray solutions on the treated plants, therefore their toxicity was increased. Toxicity of plant oils and their mixtures, clove oil exhibited the most toxic action while lemongrass oil was the least toxic against Aphis craccivora (Koch.) and Aphis gossypii (Glov.). The oil mixtures of (castor + clove), (linseed + onion) and (cottonseed + chamomile) were the most potent against both aphid species, whereas lemongrass mixed with castor or cottonseed were the least effective. Source

Abd-Alrahman S.H.,King Saud University | Abd-Alrahman S.H.,Central Agricultural Pesticides Laboratory | Kotb G.A.M.,Agricultural Research Center | Elhalwagy M.E.A.,King Abdulaziz University | And 4 more authors.
Journal of Animal and Veterinary Advances

Fenitrothion is being increasingly used as an insecticide under intensive cultivation to control vegetables and fruits pests. The bioconcentration of fenitrothion 2 different groups of freshwater fish (Oreochromis niloticus) was investigated after 28 days of exposure. One tenth of the determined 96 h-LC50 concentrations applied to fish. Fenitrothion concentration was 3.85 mg kg-1 and the Bioconcentration Factors (BCFs) after the experimental period 28 days 675.43 for whole fish body while the concentration was 1.51 mg kg-1 with respective BCFs was reached 264.91 for muscle tissue were 1.61 after daily exposure during the experimental time 28 days. Results concluded that the fenitrothion has ability to fenitrothion bioconcentration factor was high in whole fish compared to muscle tissue. © Medwell Journals, 2013. Source

ElShafei G.M.S.,Ain Shams University | El-Said M.M.,Central Agricultural Pesticides Laboratory | Attia H.A.E.,Central Agricultural Pesticides Laboratory | Mohammed T.G.M.,Central Agricultural Pesticides Laboratory
Industrial Crops and Products

In this study, we have used the essential oils Eucalyptus (E), Linalool (L) and Marjoram (M) to prepare several formulations of w/o/w double emulsion type. Span 80 and Tween 80 were the lipophilic and hydrophilic emulsifiers, respectively, with xanthan gum as thickener. Successful formulations showed stability at room temperature and 4 °C for 30 days. These formulations showed also water-dilution tolerance, and retained more than 70% of electrolyte included in the internal aqueous phase. Pesticide activity of the used oils against four fungi has been remarkably increased, based on the value of EC50, upon using in the form of w/o/w double emulsion. We have recorded up to 70% loss in required EC50 in most cases. The absence of organic solvents, unlike many common pesticide formulations, the aqueous pool embracing the environmentally safe oils and the property of controlled electrolyte release make such formulations attractive to be the subject of further more detailed studies. © 2009 Elsevier B.V. All rights reserved. Source

Sadeek S.A.,Zagazig University | El-Attar M.S.,Zagazig University | El-Lattif N.S.A.,Central Agricultural Pesticides Laboratory
Bulletin of the Chemical Society of Ethiopia

Some new solid complexes [CrCl3(L)3]×6H 23O, [FeCl3(L)3]×6H2O and [Cu(CH 3COO)2(L)3]×2H2O have been synthesized quantitatively by the interactions of 2-methyl-quinazolinone (L) with CrCl3.6H23O, FeCl3.6H2O and Cu(CH3COO)2. 2H2O in a mixture of an ethanol-bidistilled water (1:1), at 60 °C. They were characterized by melting point, molar conductivity, magnetic moment, elemental analysis, infrared spectra and thermal analyses. The results supported the formation of the complexes and indicated that the ligand reacted as a monodentate ligand bound to the metal ion through the oxygen atom. The antifungal activity of the free ligand and their metal complexes were evaluated against several species, such as Fusarium solani, Rizoctonia solani, Sclortium rolfsii and Botryodiplodia and they showed a good antifungal activity to some selected fungal strain as compared with free ligand. © 2014 Chemical Society of Ethiopia. Source

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