Centers Prof. Zech Bregenz GmbH

Austria

Centers Prof. Zech Bregenz GmbH

Austria
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Wirleitner B.,Centers Prof. Zech Bregenz GmbH | Vanderzwalmen P.,Centers Prof. Zech Bregenz GmbH | Stecher A.,Centers Prof. Zech Bregenz GmbH | Zech M.H.,Centers Prof. Zech Bregenz GmbH | And 3 more authors.
Reproductive BioMedicine Online | Year: 2010

The elucidation of the metabolic requirements of human embryos in vivo or in vitro remains, despite being intensively investigated, a work in progress. The adoption of extended embryo culture to the blastocyst stage during the last decade has entailed new challenges. With the increased attention to culture media formulations, more evidence on the sensitivity of embryos to their early environmental conditions is accumulating which might affect phenotype and developmental potential. A retrospective study was conducted that comprised 286 IVF cycles to evaluate the effect of two different culture media on blastocyst development and pregnancy outcome. Embryos were either cultured in a one step or a sequential medium. Higher fertilization rates and augmented blastocyst rates as well as higher implantation rates were observed when embryos were cultured in one step medium (P < 0.05). Interestingly, the transfer of two embryos where one embryo was cultured in either medium resulted in a significantly higher rate of twin pregnancies. Although multiple pregnancies should be avoided in assisted reproduction treatment to reduce risks for offspring and mother, this higher frequency of twin pregnancies resulting from the transfer of embryos derived from different culture media suggests that each embryo makes individual demands on its early environment. Despite intensive research, the metabolic requirements of human embryos developing in vivo or cultured in vitro are still not completely understood. Questions relating to the proper environment for embryos cultured in vitro are becoming increasingly important with the more widespread use nowadays of extended embryo culture to the blastocyst stage. Choice of the optimal culture medium has also become more important as accumulating evidence suggests that the early embryo is sensitive to the environmental conditions it experiences and that these may result in changes both to the phenotype and the embryo's potential to develop. We conducted a retrospective study comprising 286 IVF cycles to evaluate the effect of two different culture media on embryo development to the blastocyst stage and pregnancy outcome. Results showed higher rates of fertilization, blastocyst formation and implantation when a one-step medium was used for embryo culture. Interestingly, when two embryos were transferred where one of the embryos had been cultured in the one-step medium and the second in the sequential medium, a significantly higher rate of twin pregnancies was observed. The higher frequency of twin pregnancies resulting from the transfer of embryos derived from different culture media might suggest that each embryo makes individual demands on its early environment. © 2010, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.


PubMed | Centers Prof. Zech Bregenz GmbH
Type: Journal Article | Journal: Reproductive biomedicine online | Year: 2010

The elucidation of the metabolic requirements of human embryos in vivo or in vitro remains, despite being intensively investigated, a work in progress. The adoption of extended embryo culture to the blastocyst stage during the last decade has entailed new challenges. With the increased attention to culture media formulations, more evidence on the sensitivity of embryos to their early environmental conditions is accumulating which might affect phenotype and developmental potential. A retrospective study was conducted that comprised 286 IVF cycles to evaluate the effect of two different culture media on blastocyst development and pregnancy outcome. Embryos were either cultured in a one step or a sequential medium. Higher fertilization rates and augmented blastocyst rates as well as higher implantation rates were observed when embryos were cultured in one step medium (P<0.05). Interestingly, the transfer of two embryos where one embryo was cultured in either medium resulted in a significantly higher rate of twin pregnancies. Although multiple pregnancies should be avoided in assisted reproduction treatment to reduce risks for offspring and mother, this higher frequency of twin pregnancies resulting from the transfer of embryos derived from different culture media suggests that each embryo makes individual demands on its early environment.

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