Wang Q.,Nanchang Hangkong University |
Chen L.,Nanchang Hangkong University |
Zhuang T.,Center Testing International Corporation
Synthesis and Reactivity in Inorganic, Metal-Organic and Nano-Metal Chemistry | Year: 2012
The authors investigated the rheological, mechanical, and crystallization properties of nanocomposites consisting of SiO 2 modified by aluminate coupling agent and high-density polyethylene (HDPE). The scanning electron microscope photographs showed that the SiO 2 nanoparticles modified by aluminate coupling agent were evenly dispersed in the HDPE matrix without any aggregation. All the results indicate that the SiO 2 nanoparticles as a nucleation agent induced the formation of the smaller crystal sizes and higher crystallinity of polyethylene in HDPE/modified SiO 2 nanocomposites. The nanocomposites revealed excellent shear thinning behavior at high frequency and the mechanical properties could enhance at the same time. © 2012 Copyright Taylor and Francis Group, LLC.
Gao X.,Zhejiang University |
Kong L.,Zhejiang Chinese Medical University |
Lu X.,Lishui Peoples Hospital |
Zhang G.,Zhejiang University |
And 9 more authors.
PLoS ONE | Year: 2014
Paraspeckle protein 1 (PSPC1) was first identified as a structural protein of the subnuclear structure termed paraspeckle. However, the exact physiological functions of PSPC1 are still largely unknown. Previously, using a proteomic approach, we have shown that exposure to cisplatin can induce PSPC1 expression in HeLa cells, indicating the possible involvement for PSPC1 in the DNA damage response (DDR). In the current study, the role of PSPC1 in DDR was examined. First, it was found that cisplatin treatment could indeed induce the expression of PSPC1 protein. Abolishing PSPC1 expression by siRNA significantly inhibited cell growth, caused spontaneous cell death, and increased DNA damage. However, PSPC1 did not colocalize with γH2AX, 53BP1, or Rad51, indicating no direct involvement in DNA repair pathways mediated by these molecules. Interestingly, knockdown of PSPC1 disrupted the normal cell cycle distribution, with more cells entering the G2/M phase. Furthermore, while cisplatin induced G1/S arrest in HeLa cells, knockdown of PSPC1 caused cells to escape the G1/S checkpoint and enter mitosis, and resulted in more cell death. Taken together, these observations indicate a new role for PSPC1 in maintaining genome integrity during the DDR, particularly in the G1/S checkpoint. © 2014 Gao et al.
Zhang Y.,Donghua University |
Wu X.Y.,Shanghai Entry Exit Inspection and Quarantine Bureau |
Wang L.L.,Donghua University |
Wang L.L.,Center Testing International Corporation |
Ding X.M.,Donghua University
Water Science and Technology | Year: 2014
Industrial production of apparel consumes large quantity of freshwater and discharges effluents that intensify the problem of freshwater shortage and water pollution. The industrial water footprint (IWF) of a piece of apparel includes the water footprint (WF) of the fabric, apparel accessories (e.g. zipper, fastener, sewing thread) and industrial production processes. The objective of this paper is to carry out a pilot study on IWF accounting for three kinds of typical zipper (i.e. metal zipper, polyethylene terephthalate (PET) zipper and polyoxymethylene copolymer (Co-POM) zipper) that are commonly used for apparel production. The results reveal that product output exerts a remarkable influence on zipper's average IWF. Metal zipper has the largest IWF and followed by Co-POM zipper and PET zipper. Painting, dyeing and primary processing are the top three water-consuming processes and contribute about 90% of the zipper's IWF. Painting consumes the largest amount of freshwater among all processes and occupies more than 50% of the zipper's IWF. In addition, the grey water footprint (WFgrey) provides the greatest contribution, more than 80%, to the zipper's IWF. Based on these results, this paper also provides several strategies aimed at water economization and pollution reduction during industrial production of zipper. © IWA Publishing 2014.
Jiang Y.,Zhejiang University |
Jiang Y.,Center Testing International Corporation |
Zhang X.-Y.,Zhejiang University |
Sun L.,Hangzhou Normal University |
And 5 more authors.
Environmental Toxicology and Pharmacology | Year: 2012
Methyl methanesulfonate (MMS) has been shown to induce apoptosis in various cell types through p53-dependent pathways. Nevertheless, pharmacological and genetic blockade of p53 functions results in similar or delayed sensitivity to MMS treatment, suggesting the presence of p53-independent apoptotic mechanisms. To understand the p53-independent mechanisms that are engaged during MMS-induced apoptosis, we established MMS-induced apoptotic cell models using p53-deficient H1299 and Hep3B cells. Our results demonstrated that MMS at concentrations of 50, 100, 200, 400 and 800. μM induced the formation of gammaH2AX foci, and that at higher concentrations, 400 and 800. μM, MMS treatment led to apoptosis in the two cell lines. This apoptotic cell death was concurrent with the loss of mitochondrial membrane potential, nuclear-cytosolic translocation of active caspase 2, release of cytochrome c from mitochondria, and the cleavage of caspase 9, caspase 3 and PARP. However, MMS-induced DNA damage failed to stabilize the p53 family members TAp73 and DNp73. These results demonstrated a p53- and p73-independent mechanism for MMS-induced apoptosis that involves the nuclear-cytosolic translocation of active caspase 2 as well as the mitochondria-mediated pathway. © 2012 Elsevier B.V.
Wolfman H.L.,Center Testing International Corporation
Light and Engineering | Year: 2010
Solid-state lighting (SSL) technologies today are changing and improving rapidly. Products arriving on the market exhibit a wide range of performance and reliability variability. There is a need for both of the following: Standards to be used by industry in SSL product development to provide consistency among products and interchangeability where needed; Reliable, unbiased product performance information to foster the developing market for highperformance SSL products by providing assurance to users that products do indeed meet their performance claims. This article will cover both the US Department of Energy (DOE) facilitated LED standards development work and the DOE CALiPER test program for LED Lamps and Luminaires. The presentation will demonstrate how these programs are accelerating lighting energy savings by bringing factual information to the marketplace through the use of standards and creditable test programs. Although these programs are US focused, the SSL products are for the most part global in nature and the information gleamed from the US DOE programs is applicable on a global basis.