Center Technique Of Conservation Des Produits Agricoles Ctcpa

Avignon, France

Center Technique Of Conservation Des Produits Agricoles Ctcpa

Avignon, France

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Goncalves A.,French National Institute for Agricultural Research | Goncalves A.,French Institute of Health and Medical Research | Goncalves A.,Aix - Marseille University | Margier M.,French National Institute for Agricultural Research | And 13 more authors.
Food Chemistry | Year: 2016

Enriching oils, such as olive oil, could be one solution to tackle the worldwide epidemic of Vitamin D deficiency and to better fit with omega 3 (DHA) recommendations. However, data regarding the interactions occurring at the intestinal level between Vitamin D and phenols from olive oil are scarce. We first determined the effect of polyphenols from a virgin olive oil, and a virgin olive oil enriched with DHA, on Vitamin D absorption in rats. We then investigated the effects of 3 main olive oil phenols (oleuropein, hydroxytyrosol and pinoresinol) on Vitamin D uptake by Caco-2 cells. The presence of polyphenols in the olive oil supplemented with DHA inhibited Vitamin D postprandial response in rats (-25%, p < 0.05). Similar results were obtained with a mix of the 3 polyphenols delivered to Caco-2 cells. However, this inhibitory effect was due to the presence of pinoresinol only. As the pinoresinol content can highly vary between olive oils, the present results should be taken into account to formulate an appropriate oil product enriched in Vitamin D. © 2016 Elsevier Ltd. All rights reserved.


Tagliaferri C.,French National Institute for Agricultural Research | Tagliaferri C.,Clermont University | Davicco M.-J.,French National Institute for Agricultural Research | Davicco M.-J.,Clermont University | And 16 more authors.
PLoS ONE | Year: 2014

As the Mediterranean diet (and particularly olive oil) has been associated with bone health, we investigated the impact of extra virgin oil as a source of polyphenols on bone metabolism. In that purpose sham-operated (SH) or ovariectomized (OVX) mice were subjected to refined or virgin olive oil. Two supplementary OVX groups were given either refined or virgin olive oil fortified with vitamin D3, to assess the possible synergistic effects with another liposoluble nutrient. After 30 days of exposure, bone mineral density and gene expression were evaluated. Consistent with previous data, ovariectomy was associated with increased bone turnover and led to impaired bone mass and micro-architecture. The expression of oxidative stress markers were enhanced as well. Virgin olive oil fortified with vitamin D3 prevented such changes in terms of both bone remodeling and bone mineral density. The expression of inflammation and oxidative stress mRNA was also lower in this group. Overall, our data suggest a protective impact of virgin olive oil as a source of polyphenols in addition to vitamin D3 on bone metabolism through improvement of oxidative stress and inflammation. © 2014 Tagliaferri et al.


PubMed | Center Technique Of Conservation Des Produits Agricoles Ctcpa, Aix - Marseille University and French National Institute for Agricultural Research
Type: | Journal: Food chemistry | Year: 2016

Enriching oils, such as olive oil, could be one solution to tackle the worldwide epidemic of vitamin D deficiency and to better fit with omega 3 (DHA) recommendations. However, data regarding the interactions occurring at the intestinal level between vitamin D and phenols from olive oil are scarce. We first determined the effect of polyphenols from a virgin olive oil, and a virgin olive oil enriched with DHA, on vitamin D absorption in rats. We then investigated the effects of 3 main olive oil phenols (oleuropein, hydroxytyrosol and pinoresinol) on vitamin D uptake by Caco-2 cells. The presence of polyphenols in the olive oil supplemented with DHA inhibited vitamin D postprandial response in rats (-25%, p<0.05). Similar results were obtained with a mix of the 3 polyphenols delivered to Caco-2 cells. However, this inhibitory effect was due to the presence of pinoresinol only. As the pinoresinol content can highly vary between olive oils, the present results should be taken into account to formulate an appropriate oil product enriched in vitamin D.


Ben Said A.,CEA Marcoule Nuclear Site | Ben Said A.,CNRS Laboratory for Polymer Materials Engineering | Guinot C.,Center Technique Of Conservation Des Produits Agricoles Ctcpa | Ruiz J.-C.,CEA Marcoule Nuclear Site | And 4 more authors.
Journal of Supercritical Fluids | Year: 2016

Supercritical CO2 (SCCO2) extraction has been employed to remove model molecules (surrogates) which simulate the real and potential contaminants which can be present into recycled post-consumer polypropylene, limiting the recyclability for food contact applications. Different substances covering a wide range of molecular weights were extracted and evaluated. The effects of contaminant molecular structure and several processing parameters (pressure, temperature, extraction time, solvent flow rate, and matrix shape and size) upon extraction rate were investigated. The operating conditions studied were: pressure ranges of 100-300 bar, temperature ranges of 50-90 °C, indicating CO2 densities varying from 0.20 to 0.87 g/mL, and solvent flow rate ranges of 60-160 mL/min. Two shapes of contaminated material were studied: pellets and films (thickness ranges of 100-300 μm). Quantitative results for extraction kinetics have been obtained by gas chromatography. High extraction yields (100%) were achieved with films at restricted extraction times and, likewise, with pellets at reasonable extraction times. Operating conditions of 200 bar, 90 °C and 7.5 h of extraction were sufficient to achieve a complete extraction from pellet matrix (2.5 mm of diameter), even for the heaviest contaminant (807 g/mol). © 2015 Elsevier B.V. All rights reserved.


Anouar B.S.,CEA Marcoule Nuclear Site | Anouar B.S.,CNRS Laboratory for Polymer Materials Engineering | Guinot C.,University of Lyon | Ruiz J.-C.,CEA Marcoule Nuclear Site | And 4 more authors.
Journal of Supercritical Fluids | Year: 2015

Throughout their lifecycle or first use, polyolefins can be exposed to contaminating media which limit their recyclability, especially in food industry, such as packaging. Supercritical carbon dioxide (SCCO2) extraction in dynamic mode is studied as a possible method to purify post-consumer polyolefins. As a first approach, the strategy was to remove the well-known conventional additives (i.e., antioxidant) which are already present in food grade polypropylene (PP) and linear low density polyethylene (LLDPE). The extraction yields and kinetics have been obtained by gas chromatography. A systematic study of the influence of the shape (pellets or films) and the thickness of the materials shows that it is possible to increase the speed of extraction with a thinner material: high yields (100%) are obtained with films at 300 bar, 50°C and 5 min of extraction. Finally, the potential of SCCO2 extraction was compared to that of traditional liquid extraction with methylene chloride. © 2015 Elsevier B.V. All rights reserved.


Ogier N.,Laboratoire Lescuyer | Amiot M.-J.,Aix - Marseille University | George S.,Center Technique Of Conservation Des Produits Agricoles Ctcpa | Maillot M.,Aix - Marseille University | And 6 more authors.
European Journal of Nutrition | Year: 2013

Purpose: Red yeast rice (RYR), sugar cane-derived policosanols (SCdP) and artichoke leaf extracts (ALEs) are currently incorporated alone or in combination into dietary supplements for their potential low-density-lipoprotein cholesterol (LDL-cholesterol)-lowering effects. Yet, there is no information supporting the efficacy of this association on the reduction in LDL-cholesterol. The main objective of this study was to investigate the effects of a new dietary supplement (DS) with RYR, SCdP and ALEs on LDL-cholesterol. Methods: In a double-blind, randomized, parallel controlled study, 39 subjects from 21 to 55 years with moderate hypercholesterolemia without drug treatment were assigned to 2 groups and then consumed either a DS containing RYR, SCdP and ALEs or a placebo over a 16-week period. Plasma concentrations of lipids [LDL-cholesterol, total cholesterol (TC), high-density-lipoprotein cholesterol (HDL-cholesterol), triacylglycerols (TG)] and plasma levels of vitamins C and E, total polyphenols and malondialdehyde were determined at baseline and after 4, 8, 12 and 16 weeks. Results: LDL-cholesterol and TC were reduced by, respectively, 21.4 % (95 % CI, -13.3 to -24.9 %, p < 0.001) and 14.1 % (95 % CI, -10.1 to -18.0 %, p < 0.001) at week 16 in the DS group compared with baseline. Similar results were obtained at weeks 4, 8 and 12. TG decreased by 12.2 % after 16 weeks in the DS group (95 % CI: -24.4 to -0.1 %, p < 0.05). For the vitamin E/TC ratio, a difference was observed between groups at week 16 (p < 0.05). Other parameters were not modified. Conclusions: Daily consumption of this new DS decreased LDL-cholesterol and TC and is therefore an interesting, convenient aid in managing mild to moderate hypercholesterolemia. © 2012 Springer-Verlag.

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