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Sie R.S.,University dAbobo Adjame | Sie R.S.,University of Western Brittany | Charles G.,University of Western Brittany | Charles G.,European University of Brittany | And 5 more authors.
Australian Journal of Crop Science | Year: 2010

A significant work about callus induction and somatic embryogenesis was realized for Hibiscus sabdariffa. Two genotypes (Hibiscus sabdariffa var. sabdariffa and Hibiscus sabdariffa var. altissima), 2 sugars (sucrose and glucose) and three concentrations (1 %, 2%, 3%) of each sugar, 3 explant types (root, hypocotyl, cotyledon) were used for tissue culture. Fourteen combinations of plant growth regulators (PGRs) in MS medium and five combinations of PGR in Driver and Kuniyuki (DKW) medium were tested on hypocotyl and cotyledon for callus and somatic embryo formation. The PGR combinations used with MS medium were naphthaleneacetic acid/kinetin (NAA/KIN), 2,4-dichlorophenoxyacetic acid/kinetin (2,4-D/KIN), and naphthaleneacetic acid/6-benzylaminopurine (NAA/BA) and those used with DKW medium were 2,4-dichlorophenoxyacetic acid/thidiazuron (2,4-D/TDZ). Callus formation was initiated on both genotypes with all concentrations of both sugars and PGRs and in all explant types. The best results for callus induction were achieved with 3% sucrose and the hypocotyl and cotyledon explants. Somatic embryos were obtained with DKW medium supplemented with 4 mg/l 2,4-D + 1 mg/l TDZ and 1 mg/l 2,4-D + 0,5 mg/l TDZ. Source

Youssao I.A.K.,University Abomey Calavi | Tobada P.C.,University Abomey Calavi | Koutinhouin B.G.,University Abomey Calavi | Dahouda M.,University Abomey Calavi | And 7 more authors.
African Journal of Biotechnology | Year: 2010

The study of the phenotypic characterisation and molecular polymorphism of local chicken populations was carried out in Benin on 326 chickens of the Forest ecological area and 316 of the Savannah ecological area, all were 7 months old at least. The collection of blood for the molecular typing was achieved on 121 indigenous chickens of which 60 from the Savannah ecological area and 61 from the Forest ecological area. The genotyping was carried out for 22 microsatellite loci. Weight and body measures of the Savannah chickens were significantly higher (P < 0.001) than those of the Forest chickens. In the Savannah ecological area, the most frequent plumage colours were the black (22.15%), the white (19.62%), the coppery black (7.59%) and the golden partridge (7.59%). In the Forest area, the fawn (15.34%), the black (10.43%), the white (6.8%), the silver white (6.8%) and the golden partridge (6.75%) were the dominant feather colours. Thus, phenotypic characterisation showed significant differences between Savannah and Forest local chickens. The FST calculated between the Savannah and Forest populations revealed a low genetic differentiation and the dendogram showed that Savannah and Forest chickens were quite intermingled. In conclusion, local populations from Savannah and Forest area may be considered as ecotypes, but not as two distinct breeds. © 2010 Academic Journals. Source

Obouayeba A.P.,Felix Houphouet-Boigny University | Djyh N.B.,Felix Houphouet-Boigny University | Diabate S.,Center National Of Recherche Agronomique | Djaman A.J.,Felix Houphouet-Boigny University | And 3 more authors.
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2014

Roselle (Hibiscus sabdariffa L.) is known for delicacy and also for medicinal properties. The present work aim to study the phytochemical screening of Hibiscus sabdariffa for various medicinally important compounds and their quantification. The results showed that alkaloids, anthocyanins, flavonoids, saponins, steroids, sterols and tannins are present in petals of the H. sabdariffa. Anthocyanin content was highest while the contents of phenols and flavonoids were lowest. HPLC analysis revealed two phenolic acids, 16 flavonoids and four anthocyanins in petal of H. sabdariffa. The major compounds were gossypetin, hibiscetin, quercetin and sabdaretin (flavonoids) while delphinidin 3-O-sambubioside and cyanidin 3-O-sambubioside were the major anthocyanins. The antioxidant activity of the investigated extract has a scavenging ability of DPPH radical scavenging activity (around 97 %). The IC50 values of H. sabdariffa extract was 0.24 mg/ml while that of ascorbic acid used as the reference control was 0.35 mg/ml. This indicated that the compounds constituting mainly petals of H. sabdariffa, such as anthocyanins, flavonoids and phenolic acid contribute to the antioxidative activity. Our findings provide evidence that the petal extract of H. sabdariffa is a potential source of natural antioxidants, and this justify its uses in folkloric medicines. Source

Kouame M.K.,University dAboboAdjame | Dietoa M.Y.,University dAboboAdjame | Da Costa S.K.,Center National Of Recherche Agronomique | Edia E.O.,University dAboboAdjame | And 2 more authors.
Journal of Natural History | Year: 2010

We examined aquatic macroinvertebrates associated with Eichhornia crassipes roots at five sampling sites in Taabo Lake. An average density of 1644 individuals (ind.)/m2 was recorded. In total, 68 macroinvertebrate taxa belonging to 34 families and 14 orders were identified. Among these, Insecta was predominant (77.94%). The highest densities were recorded at Taabo cite during both rainy and dry seasons. Taxon diversity differences were not observed among all stations according to a Kruskal-Wallis test. High densities of predators were recorded. Next most prevalent in the trophic structure were detritivores. Indicator taxa analysis, using the Indval method, showed that stations upstream of the lake were characterized by eight taxa, while 11 appeared as indicators of the station near the dyke. Predators and herbivores dominated within those indicators. Sites near bays were distinguished by 18 indicator taxa with a predominance of predators and detritivores. Conductivity, NH4 +, temperature, PO4 3-, turbidity and dissolved oxygen were parameters that strongly influenced the macroinvertebrate community. © 2010 Taylor & Francis. Source

Kouassi N.K.,Center National Of Recherche Agronomique | Wendy M.,UK Environment Agency | Boonham N.,UK Environment Agency | Smith J.,UK Environment Agency
Acta Horticulturae | Year: 2010

Cucumber mosaic virus (CMV) is the primary virus affecting commercial banana (Musa spp.) plantations in Ivory Coast, with the disease also present within nursery systems. However, currently no virus indexing of planting material is performed for banana in the country. This current study attempts to quantify levels of detection of CMV within banana material using flinders technology associates (FTA) cards in order to identify a reliable and reproducible method suitable for local conditions. A suite of PCR primers were developed against CMV coat protein open reading frame (CP ORF) sequence available on GenBank. TaqMan® assay generated comparable CT values with either FTA or cetyltrimethyl ammonium bromide (CTAB) derived templates and was able to detect CMV RNA in leaf samples. The conventional real time polymerase chain reaction (RT-PCR) assay, RNeasy and FTA derived RNA templates reliably amplified CMV, whilst CTABderived RNA gave variable results. The TaqMan® reaction was much more sensitive than the conventional RT-PCR for CMV detection in banana leaf samples. The suitability of FTA cards as a matrix for virus nucleic acid capture provides scope for its use in the transportation of banana virus samples to laboratories for diagnosis and characterisation, circumventing constraints of time-related degeneration of sample integrity. Sequence data of the CMV CP ORF region of 16 isolates from Côte d'Ivoire clustered the isolates to CMV Sub-Group IA. This is the first successful application of Real-time PCR and FTA technology for a banana virus in Africa. Source

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