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Slama N.,Center de Biotechnologie de la Technopole de Borj-Cedria | Slama N.,Center National Des Science Et Des Technologies Nucleaires Technopole Of Sidi Thabet | Mankai H.,Center de Biotechnologie de la Technopole de Borj-Cedria | Mankai H.,Center National Des Science Et Des Technologies Nucleaires Technopole Of Sidi Thabet | And 7 more authors.
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | Year: 2014

A novel actinomycete strain designated CN-207T was isolated from northern Tunisian soil. This strain exhibited potent broad spectrum antibacterial activity against clinical isolates of methicillin-resistant Staphylococcus species and several other Gram-positive and Gram-negative bacteria. Strain CN-207T developed greyish aerial mycelium and pale grey substrate mycelium on yeast extract/malt agar. The isolate produced branching vegetative mycelia with sporangiophores bearing sporangia developing at a late stage of growth. The sporangia contained smooth, non-motile spores. Chemotaxonomic characteristics of strain CN-207T were typical of the Streptomyces genus. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CN-207T belonged to the genus Streptomyces, and was most closely related to Streptomyces griseoincarnatus DSM 40274 T, Streptomyces variabilis DSM 40179T, Streptomyces labedae DSM 41446T and Streptomyces erythrogriseus DSM 40116 T. Low DNA-DNA relatedness values were recorded between strain CN-207T and its closest phylogenetic neighbours. Strain CN-207 T was also distinguished from the nearest phylogenetic neighbours using a combination of morphological and phenotypic characteristics. On the basis of its phenotypic and molecular properties, strain CN-207T is considered as a novel species of the Streptomyces genus, for which the name Streptomyces tunisiensis sp nov is proposed. The type strain is CN-207 T (=JCM 17589T = DSM 42037T). © 2013 Springer Science+Business Media Dordrecht.

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