Center La Mojonera

La Mojonera, Spain

Center La Mojonera

La Mojonera, Spain
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Toledo-Martin E.M.,Center La Mojonera | Font R.,Center La Mojonera | Obregon-Cano S.,CSIC - Institute for Sustainable Agriculture | De Haro-Bailon A.,CSIC - Institute for Sustainable Agriculture | And 2 more authors.
Molecules | Year: 2017

The potential of visible-near infrared spectroscopy to predict glucosinolates and total phenolic content in rocket (Eruca vesicaria) leaves has been evaluated. Accessions of the E. vesicaria species were scanned by NIRS as ground leaf, and their reference values regressed against different spectral transformations by modified partial least squares (MPLS) regression. The coefficients of determination in the external validation (R2VAL) for the different quality components analyzed in rocket ranged from 0.59 to 0.84, which characterize those equations as having from good to excellent quantitative information. These results show that the total glucosinolates, glucosativin and glucoerucin equations obtained, can be used to identify those samples with low and high contents. The glucoraphanin equation obtained can be used for rough predictions of samples and in case of total phenolic content, the equation showed good correlation. The standard deviation (SD) to standard error of prediction ratio (RPD) and SD to range (RER) were variable for the different quality compounds and showed values that were characteristic of equations suitable for screening purposes or to perform accurate analyses. From the study of the MPLS loadings of the first three terms of the different equations, it can be concluded that some major cell components such as protein and cellulose, highly participated in modelling the equations for glucosinolates. © 2017 by the authors.


Martinez-Valdivieso D.,Center La Mojonera | Font R.,Center La Mojonera | Blanco-Diaz M.T.,Center La Mojonera | Moreno-Rojas J.M.,Center Alameda del Obispo | And 3 more authors.
Computers and Electronics in Agriculture | Year: 2014

The potential of near-infrared reflectance spectroscopy (NIRS) for predicting total carotenoid, lutein and β-carotene contents in skin and flesh of Cucurbita pepo fruits was assessed. The carotenoid contents were performed by HPLC, and were regressed against different spectral transformations by modified partial least square (PLSm) regression. Coefficients of determination in the external validation varied from 0.81 to 0.96, which characterize those equations as having from good to excellent quantitative information. The standard deviation (SD) to standard error of prediction ratio (RPD) and range to standard error of prediction ratio (RER) were variable for the different fruit part and compounds, and showed values that were characteristic of equations suitable for screening purposes. PLSm loading plots corresponding to the first terms of the equations showed that effects of the C-H group of starch and lipids, O-H group of water, as well as protein and chlorophyll, were most important in modeling prediction equations. The use of NIRS represents an important breakthrough in breeding for improved nutritional quality of summer squash fruit. © 2014 Elsevier B.V.


PubMed | Center La Mojonera, Center Alameda del Obispo and University of Almeria
Type: Journal Article | Journal: Journal of the science of food and agriculture | Year: 2016

The characterization of internal (Brix, pH, malic acid, total phenolic compounds, ascorbic acid and total carotenoid content) and external (color, firmness and pericarp wall thickness) pepper quality is necessary to better understand its possible applications and increase consumer awareness of its benefits. The main aim of this work was to examine the feasibility of using visible/near-infrared reflectance spectroscopy (VIS-NIRS) to predict quality parameters in different pepper types. Commercially available spectrophotometers were evaluated for this purpose: a Polychromix Phazir spectrometer for intact raw pepper, and a scanning monochromator for freeze-dried pepper.The RPD values (ratio of the standard deviation of the reference data to the standard error of prediction) obtained from the external validation exceeded a value of 3 for chlorophyll a and total carotenoid content; values ranging between 2.5


Martinez-Valdivieso D.,Center La Mojonera | Gomez P.,Center La Mojonera | Font R.,Center La Mojonera | Angeles Alonso-Moraga,University of Cordoba, Spain | Rio-Celestino M.D.,Center La Mojonera
LWT - Food Science and Technology | Year: 2015

Physical and chemical characters of 22 summer squash genotypes (traditional and commercial cultivars) were evaluated in fruit using correlation and principal component analysis. External and internal fruit quality was assessed by standard parameters (fruit weight and dimensions, firmness, color, dry matter, soluble solids, pH, total acidity) and nutritional quality (individual and total carotenoid content) in fruit epicarp and mesocarp. Some of the cultivars showed distinctive and interesting characters in mesocarp, such as high soluble solid (4.7 °Brix: Cu-8 traditional cultivar), dry matter (6.6 g 100 g1: Cu-36 commercial cultivar), b-carotene (45 mg kg1 dry matter: Cu-11 traditional cultivar) and total carotenoid contents (371 mg kg1 dry matter: Cu-29 commercial cultivar). Moderate and significant positive relation was found between epicarp and mesocarp color parameter (b*) with lutein and total carotenoid contents in mesocarp (r > 0.52***), suggesting that indirect selection for high carotenoid content will be successful. Multivariable analysis allowed the grouping of variables, the most important being the a* and b* parameters (in epicarp and mesocarp), dry matter, soluble solid, total acidity, lutein, β-carotene and total carotenoid contents (in epicarp). Some cultivars may serve as a source of desirable genes to develop improved varieties that respond to the new needs of the market. © 2015 Elsevier Ltd. All rights reserved.


Villatoro-Pulido M.,Center Alameda del Obispo s n | Font R.,Center La Mojonera | Obregon-Cano S.,CSIC - Institute for Sustainable Agriculture | Moreno-Rojas R.,University of Cordoba, Spain | And 6 more authors.
Chemosphere | Year: 2013

Rocket is an important source of essential elements. However, it may also accumulate toxic elements such as metal(oids). The objectives of the present work were (i) to study the uptake of arsenic, lead, cadmium and zinc in rocket grown in contaminated soils, (ii) to establish the genotoxic and cytotoxic activities of this vegetable material, and (iii) to study the modulator role of the glucosinolate and metal contents in the genotoxic/cytotoxic activities. Lead, cadmium and zinc leaf concentrations in our study were over the concentrations allowed by the statutory limit set for metal(oid) contents in vegetables. The accessions were non genotoxic at the different concentrations studied, although one of the accessions showed the highest mutation rates doubling those of negative control. The cytotoxicity assays with HL60 human leukaemia cells showed that the tumouricide activities of rocket leaves decreased with the increasing of metal(oid) concentrations and also with the decreasing of glucosinolate concentrations in their tissues. An interaction between metal(oid)s and glucosinolate degradation products contained in rocket leaves is suggested as the main modulator agents of the biological activity of the plants grown in metal-contaminated soils. © 2013.


Martinez-Valdivieso D.,Center La Mojonera | Gomez P.,Center La Mojonera | Font R.,Center La Mojonera | Rio-Celestino M.D.,Center La Mojonera
European Food Research and Technology | Year: 2014

Mineral concentrations were determined in fruit of 34 traditional and improved genotypes of Cucurbita pepo. Genotypes belong to two subspecies, the subsp. pepo (classified into zucchini, vegetable marrow and pumpkin morphotypes) and subsp. ovifera (with three varieties: texana, ozarkana and clypeata). Phosphorus, potassium, calcium, magnesium, iron, copper, manganese, zinc and sodium were analyzed, and two distinct patterns of mineral accumulation were found to be evident by cluster analysis. Genotypes in group 1 (zucchini and pumpkin) showed the highest concentrations of total minerals (24,338-62,136 mg kg-1 dry weight) as compared to the genotypes in group 2 (vegetable marrow, var. clypeata, var. texana and var. ozarkana). Some genotypes with significant concentrations for different minerals were identified, with the genotype Cu-2 (traditional zucchini) showing the highest concentrations for K, Ca, Mg, Fe, Mn, Zn and Na (4,615, 315, 300, 4.8, 3.03, 3.83 and 9.4 mg kg-1 dry weight, respectively). The zucchini morphotype was superior to other morphotypes studied in terms of contribution to the recommended dietary allowance of mineral content for both men and women. The mineral content of C. pepo fruit reported provides a valuable material for breeding programs to generate lines with a significant long-term beneficial impact on human health. © 2014 Springer-Verlag Berlin Heidelberg.


Blanco-Diaz M.T.,Center la Mojonera | Del Rio-Celestino M.,Center la Mojonera | Martinez-Valdivieso D.,Center la Mojonera | Font R.,Center la Mojonera
Food Chemistry | Year: 2014

The food industry and plant breeding programmes require fast, clean and low-cost screening techniques for nutritional compounds determination in food matrices. This is the first report on the study of the potential of near-infrared spectroscopy (NIRS) for the prediction of antioxidant compounds in summer squash tissues collected since 2009-2012. Modified partial least-squares (MPLS) regression was used to correlate spectral information and the different antioxidant compounds in the samples. The coefficients of determination in the external validation (r2 ev) obtained were for ascorbic acid (0.77 and 0.86), chlorophyll a (0.79 and 0.66), chlorophyll b (0.86 and 0.79) and total phenolic compounds (0.65 and 0.68) in exocarp and mesocarp tissues, respectively, supporting that NIRS is able to predict in a rapid way these components for screening purposes. Major wavelengths influencing the calibration equations showed that chromophores as well as fibre components of the fruits highly participated in developing the NIR equations. © 2014 Elsevier Ltd. All rights reserved.


The food industry and plant breeding programmes require fast, clean and low-cost screening techniques for nutritional compounds determination in food matrices. This is the first report on the study of the potential of near-infrared spectroscopy (NIRS) for the prediction of antioxidant compounds in summer squash tissues collected since 2009-2012. Modified partial least-squares (MPLS) regression was used to correlate spectral information and the different antioxidant compounds in the samples. The coefficients of determination in the external validation (r(2)ev) obtained were for ascorbic acid (0.77 and 0.86), chlorophyll a (0.79 and 0.66), chlorophyll b (0.86 and 0.79) and total phenolic compounds (0.65 and 0.68) in exocarp and mesocarp tissues, respectively, supporting that NIRS is able to predict in a rapid way these components for screening purposes. Major wavelengths influencing the calibration equations showed that chromophores as well as fibre components of the fruits highly participated in developing the NIR equations.

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