Yaseen T.,Center International Of Hautes Etudes Agronomiques Mediterraneennes Ciheam Maib |
Schena L.,University of Reggio Calabria |
Nigro F.,University of Bari |
Ippolito A.,University of Bari
Phytopathologia Mediterranea | Year: 2010
A survey on Phytophthora spp. in the soils and roots of citrus groves was carried out in the main Syrian growing areas of Lattakia and Tartous. Traditional assays (selective medium with soil dilution plates) were used for pathogen detection, and molecular (PCR) assays were used for unanmbiguous identification of P. nicotianae and P. citrophthora in 38.5% of the collected samples. In both locations, P. citrophthora was the predominant species.
Ayoub F.,Center International Of Hautes Etudes Agronomiques Mediterraneennes Ciheam Maib |
Reverberi M.,University of Rome La Sapienza |
Ricelli A.,CNR Institute of Biomolecular Chemistry |
D'Onghia A.M.,Center International Of Hautes Etudes Agronomiques Mediterraneennes Ciheam Maib |
Yaseen T.,Center International Of Hautes Etudes Agronomiques Mediterraneennes Ciheam Maib
Food Additives and Contaminants - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment | Year: 2010
Aspergillus carbonarius and A. niger aggregate are the main fungal contaminants of table grapes. Besides their ability to cause black rot, they can produce ochratoxin A (OTA), a mycotoxin that has attracted increasing attention worldwide. The objective of this work was to set up a simple and rapid molecular method for the early detection of both fungi in table grapes before fungal development becomes evident. Polymerase chain reaction (PCR)-based assays were developed by designing species-specific primers based on the polyketide synthases (PKSS) sequences of A. carbonarius and A. niger that have recently been demonstrated to be involved in OTA biosynthesis. Three table grape varieties (Red globe, Crimson seedless, and Italia) were inoculated with A. carbonarius and A. niger aggregate strains producing OTA. The extracted DNA from control (non-inoculated) and inoculated grapes was amplified by PCR using ACPKS2F-ACPKS2R for A. carbonarius and ANPKS5-ANPKS6 for A. niger aggregate. Both primers allowed a clear detection, even in symptomless samples. PCR-based methods are considered to be a good alternative to traditional diagnostic means for the early detection of fungi in complex matrix for their high specificity and sensitivity. The results obtained could be useful for the definition of a "quality label" for tested grapes to improve the safety measures taken to guarantee the production of fresh table grapes. © 2010 Taylor & Francis.