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Badiou C.,University of Lyon | Badiou C.,French Institute of Health and Medical Research | Dumitrescu O.,University of Lyon | Dumitrescu O.,French Institute of Health and Medical Research | And 19 more authors.
Journal of Clinical Microbiology | Year: 2010

Staphylococcus aureus strains producing Panton-Valentine leukocidin (PVL) have been epidemiologically linked to specific human infections. To evaluate immunological tests that may be used to diagnose infections with PVL-producing strains, we prospectively collected pus, respiratory tract specimens, and joint fluid specimens from which S. aureus had been isolated in clinical laboratories in six countries. An enzyme-linked Immunosorbent assay (ELISA) and an immunochromatographic test (ICT) targeting LukS-PV were performed directly with clinical samples for the detection of PVL. The same tests were applied to S. aureus culture supernatants. The corresponding S. aureus isolates were characterized by PCR for the presence of the PVL locus (lukS-PV and lukF-PV) and the mecA gene. A total of 185 samples from 144 skin infections, 23 bone and joint infections, and 18 lower respiratory tract infections were analyzed. By PCR, 72/185 S. aureus isolates were PVL locus positive (PVL+); 28 of these were also mecA positive. PVL was detected in the supernatants of all PVL+ strains by both ELISA and an ICT, while no signal was observed with PVL-negative strains. The PVL concentrations in human clinical samples that grew PVL+ strains ranged from 0 to 399 μg/ml by ELISA. By the use of 0.015 μg/ml of PVL as a cutoff value, PVL was detected in 65/72 (90%) of the clinical samples by ELISA. The sensitivity and specificity of the ELISA test were 90% and 100%, respectively. By the ICT, PVL was detected in 57/72 (79%) of the samples, and the sensitivity and specificity of ICT were 79% and 100%, respectively. PVL is expressed by S. aureus during human infection, and a PVL-specific ELISA and ICT could be reliable tests for the diagnosis of Infections caused by PVL-producing strains. Copyright © 2010, American Society for Microbiology. All Rights Reserved. Source

Amoura S.,Center Hospitalo University Mustapha Bacha | Dubois V.,Laboratoire HLA | Bouali-Benhalima M.,Center Hospitalo University Mustapha Bacha
Revue Francophone des Laboratoires | Year: 2012

The introduction of solid phase assays for the detection of HLA antibodies in routine led to the application of new algorithms for the immunological monitoring of patients for renal transplantation and has prevented many cases of rejection, which would have been impossible to detect using complement dependent serological techniques In this work, we made a comparative study between two techniques of HLA antibody testing, ELISA and Luminex®. Luminex®'s data were analyzed in two ways, firstly with the cut-off of the supplier and then with the new thresholds established after analysis of discrepancies and identification of antibodies. Following this study, we can argue that both techniques are sensitive. However, the Luminex® technology is more specific and faster. © 2012 - Elsevier Masson SAS - Tous droits réservés. Source

Ziane H.,Center Hospitalo University Mustapha Bacha | Manageiro V.,National Institute Of Health Dr Ricardo Jorge | Manageiro V.,University of Porto | Ferreira E.,National Institute Of Health Dr Ricardo Jorge | And 3 more authors.
Journal of Microbiological Methods | Year: 2015

We developed a new sequential multiplex-PCR-based typing scheme (MPBTS) for pneumococcal capsular classification. The serogroup/type of 37 control isolates obtained by the Quellung reaction, MPBTS, and nucleotide sequencing, were fully concordant. The serogroups/types of 75 invasive isolates determined by MPBTS, presented 100% specificity and 96% sensitivity, when compared with the Quellung reaction. © 2015 Elsevier B.V. Source

Antri K.,Center Hospitalo University Mustapha Bacha | Rouzic N.,University of Lyon | Dauwalder O.,University of Lyon | Boubekri I.,Center Hospitalo University Mustapha Bacha | And 6 more authors.
Clinical Microbiology and Infection | Year: 2011

USA300 is an epidemic community-acquired methicillin-resistant Staphylococcus aureus (C-MRSA) clone in the USA, whereas the European C-MRSA clone ST80-IV has mainly a sporadic diffusion in Europe. The prevalence of European clone ST80-IV in Algeria is poorly documented. We prospectively studied S. aureus infections at Mustapha Bacha hospital in Algiers over a 20-month period. S. aureus nasal colonization was studied during a further 6-month period. The European clone ST80-IV was responsible for more than one-third of both community infections (35.7%) and hospital infections (35.8%). Panton-Valentine leukocidin (PVL)-positive MRSA isolated from hospital inpatients were resistant to multiple antibiotics, including fluoroquinolones in 44.9% of cases. The PVL-positive MRSA nasal carriage rate was high among patients and staff in the dermatology unit (8.7% and 18.5%, respectively), but low (2.7%) among patients attending the outpatient clinic. The European PVL-positive C-MRSA clone ST80-IV is widespread in the Algiers hospital and community settings. © 2010 The Authors. Journal Compilation © 2010 European Society of Clinical Microbiology and Infectious Diseases. Source

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