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Rouger K.,French National Institute for Agricultural Research | Rouger K.,University of Nantes | Larcher T.,French National Institute for Agricultural Research | Larcher T.,University of Nantes | And 37 more authors.
American Journal of Pathology | Year: 2011

Duchenne muscular dystrophy (DMD) is a genetic progressive muscle disease resulting from the lack of dystrophin and without effective treatment. Adult stem cell populations have given new impetus to cell-based therapy of neuromuscular diseases. One of them, muscle-derived stem cells, isolated based on delayed adhesion properties, contributes to injured muscle repair. However, these data were collected in dystrophic mice that exhibit a relatively mild tissue phenotype and clinical features of DMD patients. Here, we characterized canine delayed adherent stem cells and investigated the efficacy of their systemic delivery in the clinically relevant DMD animal model to assess potential therapeutic application in humans. Delayed adherent stem cells, named MuStem cells (muscle stem cells), were isolated from healthy dog muscle using a preplating technique. In vitro, MuStem cells displayed a large expansion capacity, an ability to proliferate in suspension, and a multilineage differentiation potential. Phenotypically, they corresponded to early myogenic progenitors and uncommitted cells. When injected in immunosuppressed dystrophic dogs, they contributed to myofiber regeneration, satellite cell replenishment, and dystrophin expression. Importantly, their systemic delivery resulted in long-term dystrophin expression, muscle damage course limitation with an increased regeneration activity and an interstitial expansion restriction, and persisting stabilization of the dog's clinical status. These results demonstrate that MuStem cells could provide an attractive therapeutic avenue for DMD patients. © 2011 American Society for Investigative Pathology.


Vedrine B.,Clinique Veterinaire Cauchoise | Guillemot A.,Center Hospitalier Veterinaire Atlantia | Fontaine D.,Center Hospitalier Veterinaire Atlantia | Ragetly G.R.,Urbana University | Etchepareborde S.,Center Hospitalier Veterinaire des Cordeliers
Veterinary and Comparative Orthopaedics and Traumatology | Year: 2013

Objectives: 1) To provide specific quantitative data of tibial conformation in Labrador Retrievers and Yorkshire Terriers; 2) To compare the tibial conformation of these two breeds; and 3) To compare these data with previously reported data. Methods: Mediolateral radiographs of the stifle were obtained from 30 consecutive Labrador Retrievers and 30 consecutive Yorkshire Terriers with an angle of extension of 135°. The tibial plateau angle (TPA), the angle between the tibial plateau and the patellar tendon (PTA), the Z angle, the distal tibial axis/proximal tibial axis angle (DPA), and the relative tibial tuberosity width (rTTW) were measured and compared among the two breeds. Results: The breed had a significant effect on all of the measured variables (p <0.01): Labrador Retrievers had a lower TPA (25 ± 3° compared to 30 ± 4°), a lower Z angle (58.8 ± 3.2° compared to 69.2 ± 4.5°), a lower DPA (4.5 ± 2.3° compared to 10.8 ± 4.3°), and a lower rTTW (0.74 ± 0.1 compared to 0.86 ± 0.1) than Yorkshire Terriers. The PTA was greater in Labrador Retrievers compared to Yorkshire Terriers (106.9 ± 3.9° compared to 103.7 ± 6.5°). Four correlations were found to be significant: the DPA angle was correlated with the TPA, the Z angle, and the rTTW. The TPA was also correlated with the Z angle. Clinical significance: The variation in tibial conformation between breeds should be taken into account when studying the role of each measured parameter in the pathology of cruciate disease. When the relevance of each of the aforementioned measured parameters is better understood, it may help determine the most appropriate surgical treatment. © Schattauer 2013.


Potier R.,Center Hospitalier Veterinaire Atlantia | Reineau O.,Clinique des Perrieres
Journal of Zoo and Wildlife Medicine | Year: 2015

A 14.5-yr-old female kinkajou (Potos flavus) was diagnosed with cholelithiasis after an episode of vomiting; diagnostics included biochemical analysis and abdominal ultrasound exam. Despite antimicrobial treatment, cholelithiasis led to cholecystitis. A cholecystotomy was performed to remove choleliths and inspissated bile. Morphological and spectroscopic properties of the choleliths were similar to those of gallstones from the brown pigment family and Streptococcus sp. and Escherichia coli were isolated from the bile. Biliary tract infection is directly related to pathogenesis of brown pigment gallstones. Serial ultrasound exams revealed that cholecystitis developed secondary to the presence of gallstones in the biliary tree. Despite full recovery postsurgery, the patient died 15 mo later from gallbladder necrosis. Based on the progression of this case, a cholecystectomy would be preferred over a cholecystotomy in similar cases, and the efficacy of long-acting antibiotics may not be adequate in nontarget species. Gallstones and biliary tract infection are rarely described in small domestic carnivores, and this is the first reported case in a kinkajou. Copyright 2015 by American Association of Zoo Veterinarians.


Risi E.,Center Hospitalier Veterinaire Atlantia
Reproduction in Domestic Animals | Year: 2014

Contents: Reproduction control of small mammals is challenging. The purposes are the control of fertility and the reduction of sexual behaviour, aggressiveness and odour. Moreover, some species like ferret females need to be neutered to prevent bone marrow suppression caused by hyperoestrogenism. Many methods of sterilization have been reported, including surgical and chemical techniques. This article describes the reproductive physiology of ferrets and the techniques used to control their reproduction. Some aspects of the use of long-acting deslorelin implants in rabbits and rodents are also described. © 2014 Blackwell Verlag GmbH.


PubMed | Center Hospitalier Veterinaire Atlantia
Type: | Journal: Reproduction in domestic animals = Zuchthygiene | Year: 2014

Reproduction control of small mammals is challenging. The purposes are the control of fertility and the reduction of sexual behaviour, aggressiveness and odour. Moreover, some species like ferret females need to be neutered to prevent bone marrow suppression caused by hyperoestrogenism. Many methods of sterilization have been reported, including surgical and chemical techniques. This article describes the reproductive physiology of ferrets and the techniques used to control their reproduction. Some aspects of the use of long-acting deslorelin implants in rabbits and rodents are also described.

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