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Cheng H.-Y.,Center for Vascularized Composite Allotransplantation | Al Deek N.F.,Chang Gung Memorial Hospital | Wei F.-C.,Center for Vascularized Composite Allotransplantation | Wei F.-C.,Chang Gung Memorial Hospital | Wei F.-C.,Chang Gung University
Translational Research in Biomedicine | Year: 2016

Vascularized composite allotransplantation (VCA) has demonstrated promising results and potential, expanding possibilities for defects not amenable to reconstruction by means of autologous tissue transplantation. Success in VCA relies on multidisciplinary efforts derived from different specialties such as immunology, surgery, rehabilitation, psychology, etc. Setting up a platform to integrate these professions is thus critical for successful and innovative clinical practice and in-depth basic research. In this chapter, the authors' experience in the establishment and development of a modern VCA center at Chang Gung Memorial Hospital, Taiwan, and in pioneering a successful clinical VCA - the first in Taiwan - is presented. The short- and long-term goals of the Chang Gung Memorial Hospital VCA Center, along with potential challenges and possible solutions, are also discussed. This chapter indeed provides invaluable lessons for those who are interested in pursuing and contributing to allotransplantation. © 2016 S. Karger AG, Basel.

Hsu S.-H.,Chia Nan University of Pharmacy and Science | Hsu S.-H.,Standard Chemical and Pharmaceutical Company | Wen C.-J.,Chang Gung University | Wen C.-J.,Center for Vascularized Composite Allotransplantation | And 3 more authors.
Nanomedicine | Year: 2013

Aim: The authors studied the bioimaging and delivery of drug-entrapped, nanostructured lipid carriers with quantum dots (QDs), called QDNLCs, for integrating imaging and therapy. Materials & methods: Nanostructured lipid carriers consisting of QDs, including lipophilic QDs, carboxyl-function QDs or PEG QDs were prepared. Application of the nanocarriers was evaluated by cytotoxicity, cell migration, cellular uptake, in vivo real-time tumor monitoring and drug accumulation in tumors. Results: All QDNLCs exhibited a size of 245 nm with camptothecin encapsulation of >99%. Cytotoxicity of the nanoparticles against melanoma cells was superior to that of free camptothecin. Carboxylic acid-conjugated QDNLCs (C-QDNLCs) showed the highest cell internalization and in vivo fluorescence labeling compared with the other carriers. Real-time bioimaging demonstrated that C-QDNLCs maintained signaling in tumors for at least 24 h. The camptothecin accumulation in melanomas increased by 6.4-fold after incorporation into C-QDNLCs. Conclusion: For the first time, nanostructured lipid carriers were coordinated with QDs and an anticancer drug to provide efficient tumor imaging and drug delivery. Original submitted 1 May 2012; Revised submitted 30 August 2012; Published online 5 February 201. © 2013 Future Medicine Ltd.

Wen C.-J.,Chang Gung University | Wen C.-J.,Center for Vascularized Composite Allotransplantation | Sung C.T.,University of California at Los Angeles | Aljuffali I.A.,King Saud University | And 4 more authors.
Nanotechnology | Year: 2013

Multifunctional liposomes loaded with quantum dots (QDs) and anticancer drugs were prepared for simultaneous bioimaging and drug delivery. Different formulations, including cationic, PEGylated and deformable liposomes, were compared for their theranostic efficiency. We had evaluated the physicochemical characteristics of these liposomes. The developed liposomes were examined using experimental platforms of cytotoxicity, cell migration, cellular uptake, in vivo melanoma imaging and drug accumulation in tumors. The average size of various nanocomposite liposomes was found to be 92-134 nm. Transmission electron microscopy confirmed the presence of QDs within liposomal bilayers. The incorporation of polyethylene glycol (PEG) and Span 20 into the liposomes greatly increased the fluidity of the bilayers. The liposomes provided sustained release of camptothecin and irinotecan. The cytotoxicity and cell migration assay demonstrated superior activity of cationic liposomes compared with other carriers. Cationic liposomes also showed a significant fluorescence signal in melanoma cells after internalization. The liposomes were intratumorally administered to a melanoma-bearing mouse. Cationic liposomes showed the brightest fluorescence in tumors, followed by classical liposomes. This signal could last for up to 24 h for cationic nanosystems. Intratumoral accumulation of camptothecin from free control was 35 nmol g-1; it could be increased to 50 nmol g-1 after loading with cationic liposomes. However, encapsulation of irinotecan into liposomes did not further increase intratumoral drug accumulation. Cationic liposomes were preferable to other liposomes as nanocarriers in both bioimaging and therapeutic approaches. © 2013 IOP Publishing Ltd.

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