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Wang X.,Anhui Medical University | Liu J.,No.306 Hospital of PLA | Song S.,Center for Special Medicine and Experimental Research | Xu B.,Center for Special Medicine and Experimental Research | And 2 more authors.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2014

To detect the expressions of Toll-like receptor 2 (TLR2), TLR7, TLR9 and dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN) mRNA and protein in peripheral blood mononuclear cells (PBMCs) from patients with chronic urticaria (CU). PBMCs were isolated from 20 patients with CU and 20 normal controls. The expressions of TLR2, TLR7, TLR9 and DC-SIGN mRNA were detected by real-time fluorescent quantitative PCR and the expressions of their proteins were detected by flow cytometry. The expression of DC-SIGN mRNA in PBMCs from patients with CU was significantly lower than that from normal controls (P<0.01). The expression of TLR2 protein was significantly higher (P<0.05), but the expression of DC-SIGN protein was significantly lower (P<0.05) in CU patients than those in normal controls. The expression of DC-SIGN decreases and that of TLR2 protein increases in patients with CU. Source


Tengfei Z.,Anhui Medical University | Gangqiao Z.,Beijing Institute of Radiation Medicine | Maoxing Y.,Center for Special Medicine and Experimental Research | Zhifu W.,Beijing Institute of Radiation Medicine | And 5 more authors.
Chinese Journal of Emergency Medicine | Year: 2016

Objective To explore the association between interleukin-33 polymorphisms and the susceptibility to and severity sepsis in Chinese Han. Methods A case-control study was conducted. The tagSNPs were selected from the interleukin-33 gene by linkage disequilibrium structure and the genotypes of all samples were determined by Sequenom MassARRY system. The associations with susceptibility to and phonotypes for severity of sepsis (including severe sepsis, sepsis shock and death, etc. ) were estimated by non conditional logistic regression adjusted by age, sex, smoking, drinking, chronic disease status, APACHE II score and primary disease status. Results Seven tagSNPs (rslOl 18795 C/T, rsl6924159 G/A, rsl891385 A/C, rs2210463 A/G, rs72614079 A/G, rs76864631 G/A, rs7849201 C/T) were used for final genotyping analysis. Except for rs76864631, genotype frequencies of our patients and controls both confirmed to the Hardy- Weinberg equilibrium. There was no significant association between the 6 tagSNPs and susceptibility and disease severity of sepsis, after correction for multiple comparisons (P >0.0125). Conclusions The interleukin-33 gene polymorphisms might have not a correlation with the susceptibility to and severity of sepsis in Han Chinese population. Source


Feng K.,Center for Special Medicine and Experimental Research | Lu H.-J.,Center for Special Medicine and Experimental Research | Jia H.-Y.,Center for Medical Examination | Zheng Y.-H.,Center for Special Medicine and Experimental Research | And 3 more authors.
Medical Journal of Chinese People's Liberation Army | Year: 2015

Objective To evaluate the association between RASSF1A Ala133Ser single nucleotide polymorphism (SNP) and colorectal cancer (CRC) in patients of Han population in north China. Methods RASSF1A SNP was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis in 109 colon cancer (CC) patients, 67 rectal cancer (RC) patients and 189 age and sex matched healthy people (as controls). Unconditional logistic regression was used to analyze the gene-disease association and gene-sex interaction. Results The RASSF1A Ala133Ser SNP in CRC patients had no significant difference with that in normal control samples, but the interaction of genotype and sex existed in rectal cancer, Ala/Ser +Ser/Ser genotypes of RASSF1A gene increased the risk of rectal cancer in women (OR=3.78, 95%CI 1.31-10.89). Conclusion The RASSF1A 133Ser allele is associated with rectal cancer in women, but not colon cancer. © 2015, Med J Chin PLA. All rights reserved. Source


Liu J.,CAS Technical Institute of Physics and Chemistry | Liu J.,Center for Special Medicine and Experimental Research | Wang Y.,CAS Technical Institute of Physics and Chemistry | Song S.,Center for Special Medicine and Experimental Research | And 4 more authors.
PLoS ONE | Year: 2015

Purpose: Collagen peptides (CPs) and calcium citrate are commonly used as bone health supplements for treating osteoporosis. However, it remains unknown whether the combination of oral bovine CPs with calcium citrate is more effective than administration of either agent alone. Methods: Forty 12-week-old Sprague-Dawley rats were randomly divided into five groups (n = 8) for once-daily intragastric administration of different treatments for 3 months at 3 months after ovariectomy (OVX) as follows: sham + vehicle; OVX + vehicle; OVX + 750 mg/kg CP; OVX + CP-calcium citrate (75 mg/kg); OVX + calcium citrate (75 mg/kg). After euthanasia, the femurs were removed and analyzed by dual energy X-ray absorptiometry and micro-computed tomography, and serum samples were analyzed for bone metabolic markers. Results: OVX rats supplemented with CPs or CP-calcium citrate showed osteoprotective effects, with reductions in the OVX-induced decreases in their femoral bone mineral density. Moreover, CP-calcium citrate prevented trabecular bone loss, improved the microarchitecture of the distal femur, and significantly inhibited bone loss with increased bone volume, connectivity density, and trabecular number compared with OVX control rats. CP or CP-calcium citrate administration significantly increased serum procollagen type I N-terminal propeptide levels and reduced serum bone-specific alkaline phosphatase, osteocalcin, and C-telopeptide of type I collagen levels. Conclusions: Our data indicate that combined oral administration of bovine CPs with calcium citrate inhibits bone loss in OVX rats. The present findings suggest that combined oral administration of bovine CPs with calcium citrate is a promising alternative for reducing bone loss in osteopenic postmenopausal women. Copyright: © 2015 Liu et al. Source


Si S.,Center for Special Medicine and Experimental Research | Song S.,Center for Special Medicine and Experimental Research | Hua N.,Center for Special Medicine and Experimental Research | Han H.,306th Hospital of PLA | And 4 more authors.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2016

RESULTS: Compared with control group, the weights of rats were reduced in combined group and simulated weightlessness group, but the weights of rats increased or did not change in noise group. Rats in the three groups showed thymus atrophy. The ratio of cells increased in G0/G1 phase and decreased in S and G2/M phases. The ratios of CD4(-) CD8(-), CD4(+) CD8(-) and CD4(-) CD8(+) T lymphocytes increased and CD4(+) CD8(+) T lymphocytes decreased. However, these changes occurred at different time points in different groups.CONCLUSION: Simulated weightlessness and noise have significant effects on thymus, but the severity are different. The combined factors have superimposed effects. Maybe this is one of the reasons for depressed functions of T lymphocytes in space flight.OBJECTIVE: To observe the combined effects of simulated weightlessness and noise on the cell cycles of thymocytes and cell compositions of thymus in rats and to explore the possible mechanism of immune function depression in space flight.METHODS: SD rats were stimulated by simulated weightlessness and/or noise. On the 3rd, 7th and 14th day, the rats were weighed and then killed. The thymuses were taken, weighed and cell suspensions were made. Cell cycles and compositions in thymocytes were analyzed by flow cytometry. Source

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