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Xu Q.,China National Rice Research Institute | Chen H.,Development Center for Science and Technology | Wang C.-H.,China National Rice Research Institute | Yu H.-Y.,China National Rice Research Institute | And 5 more authors.
Journal of Integrative Agriculture | Year: 2012

A total of 408 inbred rice cultivars bred in the last decade were analyzed for 24 SSR markers. The results showed the genetic diversity of indica cultivars was higher than that of japonica cultivars, and the genetic diversity of new cultivars raised in recent years was lower. Among the six rice cropping regions (RCRs) in China, genetic diversity was the highest in the central rice region (RCR-II) and the southwest rice region (RCR-III). Genetic differences among subpopulations of japonica were more complex than those in indica. Differentiation among seasonal ecotypes and RCRs in indica populations was unclear, but differentiation between RCR-II and northeast rice region (RCR-V) was more distinct for japonica cultivars. Considering the North rice region (RCR-IV) has very low genetic diversity among the tested cultivars, it is important to broaden the genetic background for future cultivars in rice breeding programs. © 2012 Chinese Academy of Agricultural Sciences.

Meng Y.,Shanghai JiaoTong University | Liu X.,Development Center for Science and Technology | Wang S.,Shanghai JiaoTong University | Zhang D.,Shanghai JiaoTong University | And 2 more authors.
Journal of Agricultural and Food Chemistry | Year: 2012

To enforce the labeling regulations of genetically modified organisms (GMOs), the application of DNA plasmids as calibrants is becoming essential for the practical quantification of GMOs. This study reports the construction of plasmid pTC1507 for a quantification assay of genetically modified (GM) maize TC1507 and the collaborative ring trial in international validation of its applicability as a plasmid calibrant. pTC1507 includes one event-specific sequence of TC1507 maize and one unique sequence of maize endogenous gene zSSIIb. A total of eight GMO detection laboratories worldwide were invited to join the validation process, and test results were returned from all eight participants. Statistical analysis of the returned results showed that real-time PCR assays using pTC1507 as calibrant in both GM event-specific and endogenous gene quantifications had high PCR efficiency (ranging from 0.80 to 1.15) and good linearity (ranging from 0.9921 to 0.9998). In a quantification assay of five blind samples, the bias between the test values and true values ranged from 2.6 to 24.9%. All results indicated that the developed pTC1507 plasmid is applicable for the quantitative analysis of TC1507 maize and can be used as a suitable substitute for dried powder certified reference materials (CRMs). © 2011 American Chemical Society.

Han L.,Chinese Academy of Agricultural Sciences | Li S.,Chinese Academy of Agricultural Sciences | Liu P.,Development Center for Science and Technology | Peng Y.,Chinese Academy of Agricultural Sciences | Hou M.,Chinese Academy of Agricultural Sciences
Annals of the Entomological Society of America | Year: 2012

The Asiatic rice borer, Chilo suppressalis (Walker) (Lepidoptera: Crambidae), is an important rice, Oryza sativa L., pest in China and difficult to control with conventional pest management. To develop and optimize integrated pest management strategies, efficient and economic artificial diet and rearing protocols are desirable. A new artificial diet based on soybean, Glycine max (L.) Merr., powder and fresh water bamboo, Zizania caduciflora (Turcz.) Hand.-Mazz, was formulated and rearing technique was developed. Fitness parameters including larval development, immature survival, pupal weight, pupation, adult emergence, egg hatchability, and oviposition were measured to evaluate the performance of C. suppressalis fed on the diet over 15 successive generations. C. suppressalis reared on the artificial diet showed better performance with shorter developmental stage, similar larval survival rate and fecundity, and heavier pupae compared with that fed on rice plants and fresh water bamboo. A positive correlation was observed between number of eggs laid per female and number of generations reared on the diet. Larval development time tended to be shortened with successive rearing on the artificial diet. These results indicated that C. suppressalis adapted well to the artificial diet and successive rearing conditions. The diet could serve as a viable alternative to natural host plants for consecutive rearing of the insect. In addition, the diet is inexpensive (US$1.5/1,000 g) and easy to make. The better preserve ability of the diet required only one diet replacement during the rearing process. The successful development of the diet and rearing technique provides a very useful tool for refining stem borer pest management techniques. © 2012 Entomological Society of America.

Guan X.,Shanghai JiaoTong University | Guo J.,Shanghai JiaoTong University | Shen P.,Development Center for Science and Technology | Yang L.,Shanghai JiaoTong University | Zhang D.,Shanghai JiaoTong University
Food Analytical Methods | Year: 2010

To develop effective alternatives for detecting genetically modified organisms (GMOs), we reported one optimized visual loop-mediated isothermal amplification (LAMP) method for the detection of exogenous DNA targets from two GM soybean events in this study. This isothermal amplification can be performed within 40 min without polymerase chain reaction (PCR) equipment and the derived LAMP products can be directly observed by naked eye employing SybrGreen I dye instead of conventional gel electrophoresis analysis. The limits of detection of these established visual LAMP assays were about 4 copies of haploid soybean genomic DNA, and which were much higher than those of reported conventional PCR assays. Furthermore, the high specificity of LAMP assays was determined. All the results demonstrated that the developed visual LAMP assays are convenient, cost-efficient, and rapid for on spot detection of GMOs. © 2010 Springer Science+Business Media, LLC.

Guo J.,Shanghai JiaoTong University | Chen L.,Shanghai Normal University | Liu X.,Development Center for Science and Technology | Gao Y.,Shanghai JiaoTong University | And 2 more authors.
Food Chemistry | Year: 2012

Currently, the detection methods with lower cost and higher throughput are the major trend in screening genetically modified (GM) food or feed before specific identification. In this study, we developed a quadruplex degenerate PCR screening approach for more than 90 approved GMO events. This assay is consisted of four PCR systems targeting on nine DNA sequences from eight trait genes widely introduced into GMOs, such as CP4-EPSPS derived from Acetobacterium tumefaciens sp. strain CP4, phosphinothricin acetyltransferase gene derived from Streptomyces hygroscopicus (bar) and Streptomyces viridochromogenes (pat), and Cry1Ab, Cry1Ac, Cry1A(b/c), mCry3A, and Cry3Bb1 derived from Bacillus thuringiensis. The quadruplex degenerate PCR assay offers high specificity and sensitivity with the absolute limit of detection (LOD) of approximate 80 target copies. Furthermore, the applicability of the quadruplex PCR assay was confirmed by screening either several artificially prepared samples or samples of Grain Inspection, Packers and Stockyards Administration (GIPSA) proficiency program. © 2011 Elsevier Ltd. All rights reserved.

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