Center for Research and Studies in Botany

Sivakasi, India

Center for Research and Studies in Botany

Sivakasi, India
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Balasubramanian D.,Center for Research and Studies in Botany | Balasubramanian D.,Indian Council of Agricultural Research | Lingakumar K.,Center for Research and Studies in Botany | Arunachalam A.,Indian Council of Agricultural Research
Taiwania | Year: 2014

A study was conducted to understand the host-parasite relationship in terms of anatomical and physiological adaptations in Morinda tinctoria Roxb. and Cassytha filiformis L. Anatomically the haustorium of Cassytha is found to have two parts, the upper haustorium and the endophyte. The former is the portion of a haustorium that lies external to the host organ, whereas the endophyte is the portion of a haustorium that penetrates host tissues. It was also observed that the host organ triggers the dedifferentiation of cortical parenchyma to develop dense cytoplasm, conspicuous nuclei and numerous starch grains and these cells are found to serve as the initials of upper haustorium. The level of Chl b was lower than Chl a and xanthophylls in Cassytha when compared to Morinda. The photosynthetic activity was measured in intact leaves/stems of both the host and parasitic plant using Chl a fluorescence induction kinetics, which revealed that the photosynthetic efficiency was very low in the infected sample as well as in the parasite stem. Over all, the reduction in the photosynthetic efficiency was correlated to the poorly developed PS II complex.


Malathi R.,Center for Research and Studies in Botany | Ganesan V.,Center for Research and Studies in Botany
International Journal of ChemTech Research | Year: 2015

We report the biological synthesis of palladium nanoparticles with the help of Sebastiania chamaelea(L.) Muell. Arg. (Family: Euphorbiaceae) leaf. Aqueous leaf extract of Sebastiania chamaelea was employed for the bioreduction of Pd2+ ions to Pd+0+. The leaf was collected from the campus of Ayya Nadar Janaki Ammal College, Sivakasi, Tamil Nadu. 10ml leaf extract was prepared, resuspended in 90ml of palladium chloride solution and it is known as reaction medium. The colour change of the reaction medium from pale yellow to dark brown during the incubation period is due to the vibrations in Surface Plasmon Resonance (SPR). It indicates the formation of palladium nanoparticles. From this reaction medium, a small aliquot of the sample was used for the characterization of palladium nanoparticles through UV-Visible (UV-Vis) Spectroscopic analysis, Fourier Transform Infra Red (FTIR) Spectral analysis, X- Ray Diffraction (XRD) analysis and Scanning Electron Microscope (SEM) with Energy Dispersive X-ray (EDX) analysis.The SPR with λ max at 475nm and the absorbance was raised up to 0.78a.u. The FTIR analysis explains that the biomolecules responsible for the stability of palladium nanoparticles that are synthesized by the leaf broth. The XRD analysis gives the structural information of nanoparticles. The SEM and EDAX analyses confirmed the significant presence of palladium nanoparticles. The size of the particle ranged from 50 to 80 nm. Thus the synthesis of palladium nanoparticles with various sizes is achieved using the leaf broth of Sebastiania chamaelea, as a green route due to its eco-friendly nature and does not involve any toxic methods and chemical in the synthesis of palladium nanoparticles. © 2015, International Journal of ChemTech Research. All Rights Reserved


Malathi R.,Center for Research and Studies in Botany | Ganesan V.,Center for Research and Studies in Botany
International Journal of Pharma and Bio Sciences | Year: 2015

We report the environmental benign route in the synthesis of palladium nanoparticles using the aqueous leaf broth of Acalypha indica L. In this protocol, the biomolecules present in the leaf broth, reduced palladium ions into palladium nanoparticles and these biomolecules also acted as capping and stabilizing agents. The colour change in the reaction medium from pale yellow to dark brown during the incubation period indicated the synthesis of palladium nanoparticles. Further, we used the following analyses to characterize the palladium nanoparticles- UV-Visible spectroscopy, X-ray diffraction patterns (XRD), Fourier Transform Infrared Spectroscopy (FT-IR) and Scanning Electron Microscopy (SEM) with Energy Dispersive X-ray (EDX) patterns. This protocol of synthesis of palladium nanoparticles using leaf broth of Acalypha indica L. becomes environmental benign route, simple, cost effective as it does not involve any toxic chemicals. Hence, this green approach of this method makes it as an alternative route to physical and chemical methods.

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