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Sieczynski P.,Infertility Treatment Center Kriobank | Glogowski J.,University of Warmia and Mazury | Cejko B.,Polish Academy of Sciences | Grygoruk C.,Center for Reproductive Medicine Bocian
Archives of Polish Fisheries | Year: 2012

Computer Assisted Sperm Analysis (CASA) was used to evaluate and compare the sperm motility parameters of Siberian sturgeon, Acipenser baerii Brandt (n=15), and sterlet, Acipenser ruthenus L. (n=15). Analysis indicated a higher percentage of motile sperm (MOT) in the sterlet semen (44.8%) than in that of the Siberian sturgeon (41.3%). The sperm of the two species studied were similar with regard to velocity, and similarities were also noted with regard to average path velocity VAP (97.6 and 102.4 μm s-1), curvilinear velocity VCL (121.9 and 119.9 μm s-1), and straight line velocity VSL (82.5 and 86.8 μm s-1). Using the CASA system permitted making precise evaluations of the sperm motility of the two fish species studied, and thus also permitted determining the potential suitability of the semen for use in sturgeon reproduction.

Sieczynski P.,Infertility Treatment Center Kriobank | Cejko B.I.,Polish Academy of Sciences | Grygoruk C.,Center for Reproductive Medicine Bocian | Glogowski J.,Polish Academy of Sciences
Journal of Applied Ichthyology | Year: 2015

Using a computer assisted sperm analysis (CASA) system, sperm motility parameters were determined in semen of the Siberian sturgeon (Acipenser baerii) and sterlet (Acipenser ruthenus) prior to and after cryopreservation. Semen was collected from 15 Siberian sturgeon and 15 sterlet males, diluted 1:1 (sperm:freezing medium) in 23.4 mm sucrose, 0.25 mm KCl, 30 mm Tris (pH of 8.0) and 10% methanol, loaded in 0.25 ml straws and frozen over liquid nitrogen vapor. Post-thaw sperm motility was activated in 10 mm Tris buffer (pH 8.5), containing 20 mm NaCl and 2 mm CaCl2. A significant decrease in the percentage of motile sperm (MOT: from 41.3% to 25.3%), curvilinear velocity (VCL: from 121.9 to 112.2 μm s-1), amplitude of lateral head displacement (ALH: from 10.4 to 5.4 μm) and momentum (MOM: from 4591 to 1038 m s-1) was observed as effect of the cryopreservation process in the Siberian sturgeon semen. A significant decrease in the values of most CASA parameters, including MOT, VAP, VCL, VSL, ALH and MOM, were also noted following the cryopreservation of sterlet semen. In the sterlet, an additional decrease in VAP (from 102.4 to 80.3 μm s-1) and VSL (from 86.8 to 69.8 μm s-1) values was noted after cryopreservation when compared to fresh semen. Unfortunately, the cryopreservation of semen from both species led to a drop in CASA parameters, which are important to the successful fertilization of eggs. MOT and VCL parameters were the most affected in these species. They can be used as sensitive criteria to check changes in motility. © 2015 Blackwell Verlag GmbH.

Grygoruk C.,Center for Reproductive Medicine Bocian | Ratomski K.,Center for Reproductive Medicine Bocian | Kolodziejczyk M.,Bialystok Technical University | Gagan J.,Bialystok Technical University | And 4 more authors.
Fertility and Sterility | Year: 2011

Objective: To study fluid dynamics during ET. Design: Computational fluid dynamics were applied to calculate fluid velocity changes, dynamic pressure differences, and shear stress in the transferred load for the following injection speeds: 0.1, 1, 6, 12, and 20 m/sec. Setting: Academic research institute of mechanical engineering and reproduction biotechnology and private centers of reproductive medicine. Patient(s): None. Intervention(s): None. Main Outcome Measure(s): Fluid velocity, dynamic pressure, and shear stress during injection of the transferred load. Result(s): An increase of injection speed for the transferred load increased the shear stress, dynamic pressure, and velocity differences acting on the embryo. The narrowing of the catheter lumen diameter by 20% amplified the transferred fluid velocity by 78%. An embryo positioned in proximity to the catheter's wall was exposed to considerably higher shear stress, dynamic pressure, and velocity difference than an embryo in the center of the catheter's lumen. Conclusion(s): The transfer of an embryo should be conducted gently and with minimal injection speed. Any narrowing of the catheter lumen should be eliminated. Preferably the embryo should be kept far from the catheter's wall during injection of the transferred load. Copyright © 2011 American Society for Reproductive Medicine, Published by Elsevier Inc.

Zbucka-Kretowska M.,Medical University of Bialystok | Eljaszewicz A.,Medical University of Bialystok | Lipinska D.,Medical University of Bialystok | Grubczak K.,Medical University of Bialystok | And 6 more authors.
Stem Cells International | Year: 2016

Recently, murine hematopoietic progenitor stem cells (HSCs) and very small embryonic-like stem cells (VSELs) were demonstrated to express receptors for sex hormones including follicle-stimulating hormone (FSH). This raised the question of whether FSH therapy at clinically applied doses can mobilize stem/progenitor cells in humans. Here we assessed frequencies of VSELs (referred to as Lin-CD235a-CD45-CD133+ cells), HSPCs (referred to as Lin-CD235a-CD45+CD133+ cells), and endothelial progenitor cells (EPCs, identified as CD34+CD144+, CD34+CD133+, and CD34+CD309+CD133+ cells) in fifteen female patients subjected to the FSH therapy. We demonstrated that FSH therapy resulted in statistically significant enhancement in peripheral blood (PB) number of both VSELs and HSPCs. In contrast, the pattern of responses of EPCs delineated by different cell phenotypes was not uniform and we did not observe any significant changes in EPC numbers following hormone therapy. Our data indicate that FSH therapy mobilizes VSELs and HSPCs into peripheral blood that on one hand supports their developmental origin from germ lineage, and on the other hand FSH can become a promising candidate tool for mobilizing HSCs and stem cells with VSEL phenotype in clinical settings. © 2016 Monika Zbucka-Kretowska et al.

Grygoruk C.,Center for Reproductive Medicine Bocian | Sieczynski P.,Center for Reproductive Medicine Kriobank | Modlinski J.A.,Polish Academy of Sciences | Gajda B.,National Research Institute of Animal Production | And 4 more authors.
Journal of Assisted Reproduction and Genetics | Year: 2011

Purpose: The present study was designed to investigate the impact of pressure on nuclear DNA integrity in viable cells of mouse blastocysts. Methods: The blastocysts of hybrid F1 females [(C57Bl/10 J × CBA-H);N = 15] aged 2-3 months were exposed into the pressure impulse lasting ~0.021 s and characterized by a positive pressure peak of ~76 mmHg. The nuclear DNA fragmentation index of mouse blastocysts was assessed by TUNEL assay within 60 s after exposure to pressure impulse. Results: The mean nuclear DNA fragmentation index was significantly higher in the experimental group (83%) than in the control group (19.7%); p < 0.001. Conclusion(s): A low magnitude pressure impulse can induce nuclear DNA fragmentation in mouse blastocysts. The compression and decompression forces appearing during pressure fluctuations are responsible for the observed DNA shearing. © 2011 Springer Science+Business Media, LLC.

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