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Luo N.,Sichuan Agricultural University | Wang Y.Q.,Sichuan Agricultural University | Fu Y.,Center for Plant Tissue Culture | Yang Q.,Kaili University
Acta Horticulturae

The genetic relationships among daduhe loquat (Eriobotrya prinoides var. dadunensis), oakleaf loquat (Eriobotrya prinoides) and common loquat (Eriobotrya japonica) were analyzed using RAPD and ISSR markers. The results showed that the similarity coefficient between oakleaf loquat and common loquat was lower than the similarity coefficient between oakleaf loquat and daduhe loquat while the similarity coefficient between daduhe loquat and common loquat was intermediate The highest additivity was obtained when daduhe loquat was regarded as the undetermined hybrid (44.9 and 45.8%, respectively). The specific bands of oakleaf loquat and common loquat were present in daduhe loquat and it is concluded that daduhe loquat is a hybrid of oakleaf loquat and common loquat. Source

Azam F.M.S.,University of Development Alternative | Islam S.,University of Development Alternative | Rahmatullah M.,University of Development Alternative | Zaman A.,Center for Plant Tissue Culture
Acta Horticulturae

This study describes a method of clonal propagation for one of the elite cultivars of banana developed by the Bangladesh Agriculture Research Institute (BARI). The principal characters of this cultivar 'BARI -1' (AAA genome, Sapientum subgroup) are its sweetness, good smell and high yield, which have made this particular cultivar popular in Bangladesh. In vitro cormlets were formed within 2-3 weeks when meristems were carefully isolated from field-grown plants and after proper sterilization implanted in semisolid Murashige and Skoog (MS) media fortified with 2.0 mg L -2 6-benzylaminopurine (BAP). Rate of shoot proliferation increased considerably with the synergistic effect of BAP and Kinetin. Regeneration of cormlets was geared up and shoot multiplication occurred when MS was enriched with 2.0 mg L -1 BAP and 1.0 mg L -1 Kinetin. With the increase of subculture (up to 9th maximum), frequency of shoot proliferation was enhanced. Addition of 0.1 mg L -1 Indole-3-acetic acid and 10% coconut water to the medium increased shoot elongation and stimulated growth of the shoots, respectively. The micro shoots rooted well within two weeks in 1/2 MS supplemented with 0.5 mg L -1 Indole-3-butyric acid and in a few cases auto-root induction was observed when the number of subculture was beyond five. Rooting percentage and their growth were much better in liquid media in comparison to semi-solid media. Elimination of agar from the root induction media reduced the cost of production significantly. After proper acclimatization, rooted plantlets were transferred to polythene bags containing garden soil and humus (1:1). Two weeks after transplantation, 98% plants survived and flushed new leaves. No morphological variants were observed during the passage of micro-propagation. Source

YANG Q.,Sichuan Agricultural University | YANG Q.,Kaili University | Fu Y.,Center for Plant Tissue Culture | Wang Y.Q.,Sichuan Agricultural University | And 4 more authors.
Journal of Horticultural Science and Biotechnology

Field and laboratory experiments were conducted to study if rain could wash-off pollen grains from the surface of the stigma and consequently affect the efficiency of fertilisation in loquat (Eriobotrya japonica Lindl.). Flowers of the loquat cultivar, 'Dawuxing', growing in the field, were emasculated, pollinated by hand with pollen of the cultivar 'Longquan No.5', then sprayed with water to simulate the effect of rain at -8, -4, -2 h before, -0 h (immediately before hand-pollination), +0 h (immediately after hand-pollination), and 2, 4, 8, or 12 h after pollination. A control to which no water treatment was applied was also included in the assay. Meanwhile, additional assays were performed in the laboratory to improve the resolution of the field study by using earlier treatments. Here, treatments were performed by pollinating, immersing, and shaking a branch with flowers in a container of water in the laboratory -120, -60, -30, -0, +0, 30, 60, or 120 min before or after hand-pollination. One additional sample was immersed, shaken, then handpollinated, and immersed and shaken (ISPIS) again. The results showed that the numbers of pollen grains that germinated, and the percentages of fruit set, differed significantly between the -4, -2, -0, or +0 h treatments and the non-water-treated control in the field assays, especially in the case of the -0 h and +0 h treatments in which the stigmas were washed immediately before or after hand-pollination. However, the -8, 2, 4, 8, and 12 h treatments did not show significant differences from the non-treated control. On the other hand, the laboratory experiments showed that all washing treatments reduced the number of germinated pollen grains retained on the stigma, and the immersion treatments at -30, -0, +0, or 30 min as well as the additional ISPIS treatment, showed significant differences from the non-treated controls in the numbers of pollen grains retained on the stigmas. In conclusion, the results showed that adhesion of pollen grains to the stigmas in loquat was rapid and strong, and that the simulated rain treatment was not able to wash-off pollen grains completely from the stigma surface, except in the -0 h and +0 h treatments. Source

Yang Q.,Kaili University | Wang Y.Q.,Sichuan Agricultural University | Deng Q.X.,Sichuan Agricultural University | Fu Y.,Center for Plant Tissue Culture | And 3 more authors.
Acta Horticulturae

A series of experiments were carried out to determine the effects of temperature on pollen viability and stigma receptivity in E. japonica 'Dawuxing'. Flowering shoots of 'Dawuxing' loquat were held at temperatures ranging from 5 to 30°C. At low temperature (5°C), blooming and pollen grain dehiscence were delayed, but pollen viability was relatively high (55%) up to the 7th day, and ovule viability exceeded 90%. Blooming and pollen grain dispersion were promoted at temperature of 25-30°C, resulting in faster pollen germination but decreased stigma receptivity and viability (6.39%) of pollen grains cultured at 30°C, 7 days after anthesis (DAA). Stigma receptivity decreased in flowers at 30°C and 3 DAA, and there was no stigma receptivity at 5 DAA. These results indicated that flowers at the balloon stage might achieve pollination and fertilization upon weather improvement after cold and wet conditions. Source

Wang Y.Q.,Sichuan Agricultural University | Fu Y.,Center for Plant Tissue Culture | Yang Q.,Kaili University | Deng Q.X.,Sichuan Agricultural University | And 3 more authors.
Journal of Horticultural Science and Biotechnology

A collection of loquat germplasm from China, Japan, Spain, Italy, and the USA, consisting of 41 accessions (including eight wild species and 33 cultivars), was analysed using inter simple sequence repeat (ISSR) markers. Twenty primers selected from 100 candidate primers were applied to the PCR amplifications, producing 436 bands, of which 392 bands (89.9%) were polymorphic. The average value of the effective number of alleles (Ne), Nei's gene diversity (H), and Shannon's information index (I) were 1.333, 0.209 and 0.332, respectively. There were 33 specific markers for the collection, but no markers were specific for the wild species or for the cultivars which flowered in Spring or in AutumnWinter. According to the dendrogram, Spring-flowering accessions could not be clustered into one Group. The collection was divided into non-cultivated types and cultivated types, but the latter could not be classified further based upon a single trait. The experimental data are used to discuss genetic diversity and flowering-time as a criterion for the classification of wild species and cultivars, and the taxonomic status of Eriobotrya prinoides var. dadunensis. Source

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