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Yan S.-J.,Beijing Normal University | Yan S.-J.,Beijing Area Major Laboratory of Protection and Utilization of Traditional Chinese Medicine | Yan S.-J.,Center for Natural Medicine Engineering | Wang L.,Beijing Normal University | And 26 more authors.
Journal of Agricultural and Food Chemistry | Year: 2012

Accumulation of advanced glycation end products (AGEs) has been implicated in the development of diabetic nephropathy. We investigated the effects of Pu-erh tea on AGE accumulation associated with diabetic nephropathy. Although it did not affect blood glucose levels and insulin sensitivy, Pu-erh tea treatment for 8 weeks attenuated the increases in urinary albumin, serum creatinine, and mesangial matrix in db/db mice. We found that Pu-erh tea prevented diabetes-induced accumulation of AGEs and led to a decreased level of receptor for AGE expression in glomeruli. Both production and clearance of carbonyl compounds, the main precursor of AGE formation, were probably attenuated by Pu-erh tea in vivo independent of glyoxalase I expression. In vitro, HPLC assay demonstrated Pu-erh tea could trap methylglyoxal in a dose-dependent manner. Our study raises the possibility that inhibition of AGE formation by carbonyl trapping is a promising approach to prevent or arrest the progression of diabetic complications. © 2012 American Chemical Society. Source


Zhu M.,Beijing Normal University | Zhu M.,Center for Natural Medicine Engineering | Wei F.,Miaoxiang Panax notoginseng Technology Co. | Cui B.,Beijing Normal University | And 5 more authors.
Journal of Plant Resources and Environment | Year: 2014

Effect of soil with Cd adding amounts of 0. 0 (CK), 0. 1, 0. 3, 0. 6, 1. 0, 3. 0, 6. 0, 10. 0 and 30. 0 mg·kg-1 on growth and antioxidant enzyme activity of Panax notoginseng (Burk.) F. H. Chen was studied by pot experiment method, and DNA damage in root-tip cell of P. notoginseng under Cd stress was analyzed by Comet assay. Results show that with rising of Cd adding amount in soil, survival rate, plant height, compound leaf number per plant, leaf area per plant and dry and fresh weights of root, stem and leaf per plant appear the trend of first increasing and then decreasing. In which, survival rate and compound leaf number per plant of all treatment groups are higher than those of the control, while plant height and leaf area per plant are higher under low adding amount of Cd and are lower under high adding amount of Cd than those of the control. And dry and fresh weights of root, stem and leaf per plant under Cd adding amount of 30. 0 mg·kg-1 are all lower than those of the control with no significant difference. With rising of Cd adding amount, SOD and CAT activities in root system appear the changing trend of "enhancing-reducing-enhancing-reducing", while overall, POD activity enhances gradually. In which, SOD activity of treatment groups with Cd adding amounts of 0. 6, 1. 0, 3. 0 and 30. 0 mg·kg-1 is extremely significant or significant lower than that of the control, while POD and CAT activities of all treatment groups are generally higher than those of the control and have extremely significant difference with the control under Cd adding amounts of 1. 0-30. 0 mg·kg-1. Comet assay result shows that there are some differences in tail length, tail DNA relative content and Olive tail moment in root-tip cell of all treatment groups, in which, these three indexes are higher under higher adding amount of Cd than those of the control with no significant difference. It is suggested that there is no obvious injury on growth, antioxidant enzyme activity and root system DNA of P. notoginseng under soil Cd stress with lower level, but there is an inhibitory action on growth and antioxidant enzyme activity and a serious injury on root-tip cell DNA under soil Cd stress with higher level. Source

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