Center for Natural Medicine Engineering

Beijing, China

Center for Natural Medicine Engineering

Beijing, China
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Li Z.,Beijing Normal University | Li Z.,Center for Natural Medicine Engineering | Yan S.-J.,Beijing Normal University | Yan S.-J.,Center for Natural Medicine Engineering | And 4 more authors.
Chinese Pharmacological Bulletin | Year: 2010

Glyoxalase I that presents in all human tissues detoxifies α-oxoaldehydes and prevents the formation of advanced glycation end products (AGEs). AGEs and their main precursor namely methylglyoxal(MG) produce cytotoxicity and have extensive relationship with diabetic complications. Therefore, to elevate glyoxalase I activity may be a new path to release such complications.


Yang Y.-F.,Beijing Normal University | Yang Y.-F.,Peking University | Yang Y.-F.,Center for Natural Medicine Engineering | Li Z.,Beijing Normal University | And 11 more authors.
Chinese Pharmacological Bulletin | Year: 2012

Aim: To establish a HPLC-MS-MS method for the determination of baicalin in rat cerebrospinal fluid(CSF) and investigate the pharmacokinetics of baicalin and compatibility with Panax Notoginsenosides (PNS). Methods: After baicalin and compatibility of BA-PNS were given by intravenous administration, the concentration of baicalin in rat CSF was determined by HPLC-MS-MS. Chromatographic separation was performed on an Agilent Eclipse XDB-C 18 (4.6 mm x 150 mm, 5 μm) within 7 min, with the mobile gradientphase of acetonitrile: 1mmol·L -1 ammonium acetate (containing 0.1% formic acid): 0.1% ammonia. The target ion transitions were m/z 445/269 (baicalin [M-1] -) and m/z 237/194 (Carbamazepine [M + 1] +). Results: Calibration curves were linear over the concentration range from 5 ∼ 200 μg·L for baicalin, with the recovery rates between 87.15% ∼ 92.73%. The pharmacokinetic parameters after a single dose of baicalin and baicalin-PNS were as follows: C max: (189.67 ± 20.13), (237.00 ± 51.86) μg·L -1; AUC 0-t: (6612.96 ± 1023.65), (5466.63 ± 267.06) μg·min· L -1; AUC 0-∞ (6930.43 ± 1060.41), (5682.13 ± 412.79) μg·min·L -1; T 1/2: (108.84 ± 45.67), (102.54 ± 38.37) min; CL: (15.39 ± 2.60), (18.32 ± 0.88) L·min -1·kg -1. Conclusion: The method is proved to be accurate and convenient to determine baicalin in rat cerebrospinal fluid. Baicalin can pass through blood-brain barrier and enter the cerebrospinal fluid. The pharmacokinetic parameters of baicalin have not changed, so PNS has no effect on the CSF pharmacokinetic of baicalin.


Wang L.,Beijing Normal University | Wang L.,Protection and Utilization of Chinese Medicine Resources of Beijing Key Laboratory | Wang L.,Center for Natural Medicine Engineering | Xin W.-F.,Beijing Normal University | And 5 more authors.
Chinese Pharmacological Bulletin | Year: 2012

Alzheimer's disease is a progressive neurodegenerative disease characterized by memory impairment and cognitive dysfunction. Accumulating evidence points to the crucial role of β-amyloid peptide as a trigger of the pathological cascade of AD. Geniposide is the main bioactive constitute of traditional Chinese medicine Gardenia jasminoides Ellis, and its aglycon is genipin. Geniposide and genipin can significantly improve the ability of learning and memory in animals with Alzheimer's disease, and exerts a combination of neuroprotective function. This review focuses on the molecular mechanisms of geniposide and genipin affecting Alzheimer' s disease and contribute to a better exploitation.


Lu C.,Beijing Normal University | Lu C.,Center for Natural Medicine Engineering | Liu H.-J.,Beijing Normal University | Liu H.-J.,Center for Natural Medicine Engineering | And 4 more authors.
Chinese Pharmacological Bulletin | Year: 2013

RAGE (receptor for advanced glycation end products) is a multiligand receptor on the cell surface. Ligand-RAGE interactions activate several signal transduction pathways that propagate cellular oxidative stress and inflammatory response. RAGE has been recognized as a key molecule in the development of severe chronic pathologies, including diabetic complications, chronic inflammation, Alzheimer's disease, cancer and so on. Blockade of ligand-RAGE interaction with sRAGE, RAGE specific antibody or other RAGE inhibitors can stop the effect that RAGE produces. It is suggested that blockade of the RAGE axis may be a new target for therapeutic intervention in the diseases mentioned above. In this article, we briefly review the relationship between RAGE ligand and disease, the signal transduction pathways activated by ligand-RAGE interactions and summarize the research progress on RAGE inhibitor.


Zhu M.,Beijing Normal University | Zhu M.,Center for Natural Medicine Engineering | Wei F.,Miaoxiang Panax notoginseng Technology Co. | Cui B.,Beijing Normal University | And 5 more authors.
Journal of Plant Resources and Environment | Year: 2014

Effect of soil with Cd adding amounts of 0. 0 (CK), 0. 1, 0. 3, 0. 6, 1. 0, 3. 0, 6. 0, 10. 0 and 30. 0 mg·kg-1 on growth and antioxidant enzyme activity of Panax notoginseng (Burk.) F. H. Chen was studied by pot experiment method, and DNA damage in root-tip cell of P. notoginseng under Cd stress was analyzed by Comet assay. Results show that with rising of Cd adding amount in soil, survival rate, plant height, compound leaf number per plant, leaf area per plant and dry and fresh weights of root, stem and leaf per plant appear the trend of first increasing and then decreasing. In which, survival rate and compound leaf number per plant of all treatment groups are higher than those of the control, while plant height and leaf area per plant are higher under low adding amount of Cd and are lower under high adding amount of Cd than those of the control. And dry and fresh weights of root, stem and leaf per plant under Cd adding amount of 30. 0 mg·kg-1 are all lower than those of the control with no significant difference. With rising of Cd adding amount, SOD and CAT activities in root system appear the changing trend of "enhancing-reducing-enhancing-reducing", while overall, POD activity enhances gradually. In which, SOD activity of treatment groups with Cd adding amounts of 0. 6, 1. 0, 3. 0 and 30. 0 mg·kg-1 is extremely significant or significant lower than that of the control, while POD and CAT activities of all treatment groups are generally higher than those of the control and have extremely significant difference with the control under Cd adding amounts of 1. 0-30. 0 mg·kg-1. Comet assay result shows that there are some differences in tail length, tail DNA relative content and Olive tail moment in root-tip cell of all treatment groups, in which, these three indexes are higher under higher adding amount of Cd than those of the control with no significant difference. It is suggested that there is no obvious injury on growth, antioxidant enzyme activity and root system DNA of P. notoginseng under soil Cd stress with lower level, but there is an inhibitory action on growth and antioxidant enzyme activity and a serious injury on root-tip cell DNA under soil Cd stress with higher level.


Yan S.-J.,Beijing Normal University | Yan S.-J.,Beijing Area Major Laboratory of Protection and Utilization of Traditional Chinese Medicine | Yan S.-J.,Center for Natural Medicine Engineering | Wang L.,Beijing Normal University | And 26 more authors.
Journal of Agricultural and Food Chemistry | Year: 2012

Accumulation of advanced glycation end products (AGEs) has been implicated in the development of diabetic nephropathy. We investigated the effects of Pu-erh tea on AGE accumulation associated with diabetic nephropathy. Although it did not affect blood glucose levels and insulin sensitivy, Pu-erh tea treatment for 8 weeks attenuated the increases in urinary albumin, serum creatinine, and mesangial matrix in db/db mice. We found that Pu-erh tea prevented diabetes-induced accumulation of AGEs and led to a decreased level of receptor for AGE expression in glomeruli. Both production and clearance of carbonyl compounds, the main precursor of AGE formation, were probably attenuated by Pu-erh tea in vivo independent of glyoxalase I expression. In vitro, HPLC assay demonstrated Pu-erh tea could trap methylglyoxal in a dose-dependent manner. Our study raises the possibility that inhibition of AGE formation by carbonyl trapping is a promising approach to prevent or arrest the progression of diabetic complications. © 2012 American Chemical Society.

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